scholarly journals Detection of enzyme activity in orthotopic murine breast cancer by fluorescence lifetime imaging using a fluorescence resonance energy transfer–based molecular probe

2011 ◽  
Vol 16 (6) ◽  
pp. 066019 ◽  
Author(s):  
Metasebya Solomon ◽  
Kevin Guo ◽  
Gail P. Sudlow ◽  
Mikhail Y. Berezin ◽  
W. Barry Edwards ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Enzyme Activity ◽  
Energy Transfer ◽  
Fluorescence Resonance Energy Transfer ◽  
Fluorescence Lifetime ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Molecular Probe ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging

Automated multiwell fluorescence lifetime imaging for Förster resonance energy transfer assays and high content analysis

2015 ◽  
Vol 7 (10) ◽  
pp. 4071-4089 ◽  
Author(s):  
Douglas J. Kelly ◽  
Sean C. Warren ◽  
Dominic Alibhai ◽  
Sunil Kumar ◽  
Yuriy Alexandrov ◽  
...  
Keyword(s):  
Content Analysis ◽  
Energy Transfer ◽  
Fluorescence Lifetime ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Förster Resonance Energy Transfer ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging ◽  
High Content Analysis ◽  
Förster Resonance

An HCA-FLIM instrument is presented alongside exemplar oligomerisation, intermolecular and intramolecular FRET assays that require robust measurement of small lifetime changes.

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