Electrochemical sensor for superoxide anion radical using polymeric iron porphyrin complexes containing axial 1-methylimidazole ligand as cytochromec mimics

2005 ◽  
Vol 16 (4) ◽  
pp. 287-292 ◽  
Author(s):  
Makoto Yuasa ◽  
Kenichi Oyaizu ◽  
Aritomo Yamaguchi ◽  
Masuhide Ishikawa ◽  
Katsuya Eguchi ◽  
...  
Keyword(s):  
Electrochemical Sensor ◽  
Superoxide Anion ◽  
Anion Radical ◽  
Superoxide Anion Radical ◽  
Iron Porphyrin ◽  
Iron Porphyrin Complexes

scholarly journals Detection of Superoxide Anion Radical Using Iron Porphyrin Complexes Bound to Electropolymerized Axial Ligands

2009 ◽  
Vol 77 (11) ◽  
pp. 940-944
Author(s):  
Makoto YUASA ◽  
Tomohiro KOBAYASHI ◽  
Tetsuya AOKI ◽  
Hiroyuki FUJII ◽  
Masatsugu IGARASHI ◽  
...  
Keyword(s):  
Superoxide Anion ◽  
Anion Radical ◽  
Superoxide Anion Radical ◽  
Iron Porphyrin ◽  
Axial Ligands ◽  
Iron Porphyrin Complexes

scholarly journals Real-time monitoring of superoxide anion radical generation in response to wounding: electrochemical study

PeerJ ◽  
2017 ◽  
Vol 5 ◽  
pp. e3050 ◽  
Author(s):  
Ankush Prasad ◽  
Aditya Kumar ◽  
Ryo Matsuoka ◽  
Akemi Takahashi ◽  
Ryo Fujii ◽  
...  
Keyword(s):  
Real Time ◽  
Superoxide Anion ◽  
Anion Radical ◽  
Spinacia Oleracea ◽  
Superoxide Anion Radical ◽  
Stress Factors ◽  
Iron Porphyrin ◽  
Real Time Monitoring ◽  
Kinetic Measurements ◽  
Oxidation Current

Background The growth and development of plants is deleteriously affected by various biotic and abiotic stress factors. Wounding in plants is caused by exposure to environmental stress, mechanical stress, and via herbivory. Typically, oxidative burst in response to wounding is associated with the formation of reactive oxygen species, such as the superoxide anion radical (O2•−), hydrogen peroxide (H2O2) and singlet oxygen; however, few experimental studies have provided direct evidence of their detection in plants. Detection of O2•− formation in plant tissues have been performed using various techniques including electron paramagnetic resonance spin-trap spectroscopy, epinephrine-adrenochrome acceptor methods, staining with dyes such as tetrazolium dye and nitro blue tetrazolium (NBT); however, kinetic measurements have not been performed. In the current study, we provide evidence of O2•− generation and its kinetics in the leaves of spinach (Spinacia oleracea) subjected to wounding. Methods Real-time monitoring of O2•− generation was performed using catalytic amperometry. Changes in oxidation current for O2•− was monitored using polymeric iron-porphyrin-based modified carbon electrodes (φ = 1 mm) as working electrode with Ag/AgCl as the reference electrode. Result The results obtained show continuous generation of O2•− for minutes after wounding, followed by a decline. The exogenous addition of superoxide dismutase, which is known to dismutate O2•− to H2O2, significantly suppressed the oxidation current. Conclusion Catalytic amperometric measurements were performed using polymeric iron-porphyrin based modified carbon electrode. We claim it to be a useful tool and a direct method for real-time monitoring and precise detection of O2•− in biological samples, with the potential for wide application in plant research for specific and sensitive detection of O2•−.

A Novel Superoxide Anion Radical Nonenzymatic Electrochemical Sensor Based on PtRuCu Ternary Alloy Nanoparticles/Graphene Composite Modified Electrode

NANO ◽  
2017 ◽  
Vol 12 (01) ◽  
pp. 1750006 ◽  
Author(s):  
Jujie Ren ◽  
Miao Zhai ◽  
Min Cui ◽  
Liujia Li ◽  
Congcong Yu ◽  
...  
Keyword(s):  
Electrochemical Sensor ◽  
Ternary Alloy ◽  
Superoxide Anion ◽  
Anion Radical ◽  
High Sensitivity ◽  
Electrochemical Determination ◽  
Superoxide Anion Radical ◽  
Alloy Nanoparticles ◽  
Reduced Graphene ◽  
Electrochemically Reduced Graphene Oxide

A superoxide anion radical (O[Formula: see text] nonenzymatic electrochemical sensor based on PtRuCu ternary alloy nanoparticles/electrochemically reduced graphene oxide (ERGO) composite modified glassy carbon electrode (PtRuCu/ERGO/GCE) was developed. ERGO and PtRuCu/ERGO composites were characterized by scanning electron microscopy. The prepared PtRuCu/ERGO/GCE showed remarkable performance toward the electrocatalytic reduction of (O[Formula: see text]. The sensor possessed a high sensitivity of 1725[Formula: see text]A[Formula: see text][Formula: see text][Formula: see text]mM[Formula: see text][Formula: see text]cm[Formula: see text], a wide linear range of 7.5–330[Formula: see text][Formula: see text]M ([Formula: see text]0.993) and a low detection limit of 0.7[Formula: see text][Formula: see text]M ([Formula: see text]/[Formula: see text]), as well as excellent selectivity, reproducibility and stability, which can be attributed to the synergistic effect of ERGO and PtRuCu ternary alloy nanoparticles. Therefore, the prepared sensor is promising for electrochemical determination of O[Formula: see text].

scholarly journals Detection of Superoxide Anion Radical in a Stratum Corneum Intercellular Lipid Model Using an Electrochemical Sensor

2011 ◽  
Vol 60 (12) ◽  
pp. 647-654 ◽  
Author(s):  
Teruhisa Kaneko ◽  
Chihiro Kaise ◽  
Yoko Kimoto ◽  
Shota Suzuki ◽  
Takeshi Kondo ◽  
...  
Keyword(s):  
Stratum Corneum ◽  
Electrochemical Sensor ◽  
Superoxide Anion ◽  
Anion Radical ◽  
Superoxide Anion Radical ◽  
Intercellular Lipid

Enzyme-free electrochemical sensor based on ZIF-67 for the detection of superoxide anion radical released from SK-BR-3 cells

2019 ◽  
Vol 855 ◽  
pp. 113653 ◽  
Author(s):  
Huanhuan Zhang ◽  
Xuan Cai ◽  
Hongli Zhao ◽  
Wenqian Sun ◽  
Zhenxing Wang ◽  
...  
Keyword(s):  
Electrochemical Sensor ◽  
Superoxide Anion ◽  
Anion Radical ◽  
Superoxide Anion Radical

In vivo real-time measurement of superoxide anion radical with a novel electrochemical sensor

2009 ◽  
Vol 47 (7) ◽  
pp. 1039-1048 ◽  
Author(s):  
Motoki Fujita ◽  
Ryosuke Tsuruta ◽  
Shunji Kasaoka ◽  
Kenji Fujimoto ◽  
Ryo Tanaka ◽  
...  
Keyword(s):  
Electrochemical Sensor ◽  
Real Time ◽  
Superoxide Anion ◽  
Anion Radical ◽  
Time Measurement ◽  
Superoxide Anion Radical ◽  
Real Time Measurement
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