Guanidinopropyl end‐modified poly(ethylene glycol) to form highly compact plasmid DNA mono‐ion complexes by thermal treatment

2021 ◽  
Author(s):  
Haibo Qiao ◽  
Shoichiro Asayama
Keyword(s):  
Thermal Treatment ◽  
Ethylene Glycol ◽  
Plasmid Dna ◽  
Poly Ethylene Glycol ◽  
Poly Ethylene

Alkylimidazolium End-Modified Poly(ethylene glycol) To Form the Mono-ion Complex with Plasmid DNA for in Vivo Gene Delivery

2014 ◽  
Vol 15 (3) ◽  
pp. 997-1001 ◽  
Author(s):  
Shoichiro Asayama ◽  
Atsushi Nohara ◽  
Yoichi Negishi ◽  
Hiroyoshi Kawakami
Keyword(s):  
Gene Delivery ◽  
Ethylene Glycol ◽  
Plasmid Dna ◽  
Poly Ethylene Glycol ◽  
In Vivo Gene Delivery ◽  
Poly Ethylene

scholarly journals Preparation of thermostable poly(ethylene glycol) using thermal treatment in sealed system

2000 ◽  
Vol 11 (2) ◽  
Author(s):  
Carmem D. Cardoso ◽  
Majulaine Copetti ◽  
Leticia Schwerz ◽  
Nádia Viaro ◽  
Martha Adaime
Keyword(s):  
Thermal Treatment ◽  
Ethylene Glycol ◽  
Poly Ethylene Glycol ◽  
Poly Ethylene

Plasmid DNA partitioning and separation using poly(ethylene glycol)/poly(acrylate)/salt aqueous two-phase systems

2012 ◽  
Vol 1233 ◽  
pp. 30-35 ◽  
Author(s):  
Hans-Olof Johansson ◽  
Tiago Matos ◽  
Juliana S. Luz ◽  
Eloi Feitosa ◽  
Carla C. Oliveira ◽  
...  
Keyword(s):  
Ethylene Glycol ◽  
Plasmid Dna ◽  
Poly Ethylene Glycol ◽  
Two Phase ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
Poly Ethylene ◽  
Dna Partitioning

One-step preparation of poly(ϵ-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone) nanoparticles for plasmid DNA delivery

2008 ◽  
Vol 86A (4) ◽  
pp. 979-986 ◽  
Author(s):  
Mei Juan Huang ◽  
Ma Ling Gou ◽  
Zhi Yong Qian ◽  
Mei Dai ◽  
Xing Yi Li ◽  
...  
Keyword(s):  
Ethylene Glycol ◽  
Plasmid Dna ◽  
Dna Delivery ◽  
Poly Ethylene Glycol ◽  
One Step ◽  
Poly Ethylene

scholarly journals PEGylated Amine-Functionalized Poly(ε-caprolactone) for the Delivery of Plasmid DNA

Materials ◽  
2020 ◽  
Vol 13 (4) ◽  
pp. 898 ◽  
Author(s):  
Amin Jafari ◽  
Nika Rajabian ◽  
Guojian Zhang ◽  
Mohamed Alaa Mohamed ◽  
Pedro Lei ◽  
...  
Keyword(s):  
Ethylene Glycol ◽  
Plasmid Dna ◽  
Viral Vectors ◽  
Viral Vector ◽  
Hydrolytic Stability ◽  
Poly Ethylene Glycol ◽  
Amine Functionalized ◽  
Structures And Properties ◽  
Wide Range ◽  
Poly Ethylene

As a promising strategy for the treatment of various diseases, gene therapy has attracted increasing attention over the past decade. Among various gene delivery approaches, non-viral vectors made of synthetic biomaterials have shown significant potential. Due to their synthetic nature, non-viral vectors can have tunable structures and properties by using various building units. In particular, they can offer advantages over viral vectors with respect to biosafety and cytotoxicity. In this study, a well-defined poly(ethylene glycol)-block-poly(α-(propylthio-N,N-diethylethanamine hydrochloride)-ε-caprolactone) diblock polymer (PEG-b-CPCL) with one poly(ethylene glycol) (PEG) block and one tertiary amine-functionalized cationic poly(ε-caprolactone) (CPCL) block, as a novel non-viral vector in the delivery of plasmid DNA (pDNA), was synthesized and studied. Despite having a degradable polymeric structure, the polymer showed remarkable hydrolytic stability over multiple weeks. The optimal ratio of the polymer to pDNA for nanocomplex formation, pDNA release from the nanocomplex with the presence of heparin, and serum stability of the nanocomplex were probed through gel electrophoresis. Nanostructure of the nanocomplexes was characterized by DLS and TEM imaging. Relative to CPCL homopolymers, PEG-b-CPCL led to better solubility over a wide range of pH. Overall, this work demonstrates that PEG-b-CPCL possesses a range of valuable properties as a promising synthetic vector for pDNA delivery.

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