Preparation of monomethyl poly(ethylene glycol)-g-chitosan copolymers with various degrees of substitution: Their ability to encapsulate and condense plasmid DNA

2008 ◽  
Vol 108 (5) ◽  
pp. 2958-2967 ◽  
Author(s):  
Wei Zhang ◽  
Shirong Pan ◽  
Xuan Zhang ◽  
Xin Luo ◽  
Zhuo Du
Keyword(s):  
Ethylene Glycol ◽  
Plasmid Dna ◽  
Poly Ethylene Glycol ◽  
Poly Ethylene

Alkylimidazolium End-Modified Poly(ethylene glycol) To Form the Mono-ion Complex with Plasmid DNA for in Vivo Gene Delivery

2014 ◽  
Vol 15 (3) ◽  
pp. 997-1001 ◽  
Author(s):  
Shoichiro Asayama ◽  
Atsushi Nohara ◽  
Yoichi Negishi ◽  
Hiroyoshi Kawakami
Keyword(s):  
Gene Delivery ◽  
Ethylene Glycol ◽  
Plasmid Dna ◽  
Poly Ethylene Glycol ◽  
In Vivo Gene Delivery ◽  
Poly Ethylene

Plasmid DNA partitioning and separation using poly(ethylene glycol)/poly(acrylate)/salt aqueous two-phase systems

2012 ◽  
Vol 1233 ◽  
pp. 30-35 ◽  
Author(s):  
Hans-Olof Johansson ◽  
Tiago Matos ◽  
Juliana S. Luz ◽  
Eloi Feitosa ◽  
Carla C. Oliveira ◽  
...  
Keyword(s):  
Ethylene Glycol ◽  
Plasmid Dna ◽  
Poly Ethylene Glycol ◽  
Two Phase ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
Poly Ethylene ◽  
Dna Partitioning

One-step preparation of poly(ϵ-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone) nanoparticles for plasmid DNA delivery

2008 ◽  
Vol 86A (4) ◽  
pp. 979-986 ◽  
Author(s):  
Mei Juan Huang ◽  
Ma Ling Gou ◽  
Zhi Yong Qian ◽  
Mei Dai ◽  
Xing Yi Li ◽  
...  
Keyword(s):  
Ethylene Glycol ◽  
Plasmid Dna ◽  
Dna Delivery ◽  
Poly Ethylene Glycol ◽  
One Step ◽  
Poly Ethylene

scholarly journals PEGylated Amine-Functionalized Poly(ε-caprolactone) for the Delivery of Plasmid DNA

Materials ◽  
2020 ◽  
Vol 13 (4) ◽  
pp. 898 ◽  
Author(s):  
Amin Jafari ◽  
Nika Rajabian ◽  
Guojian Zhang ◽  
Mohamed Alaa Mohamed ◽  
Pedro Lei ◽  
...  
Keyword(s):  
Ethylene Glycol ◽  
Plasmid Dna ◽  
Viral Vectors ◽  
Viral Vector ◽  
Hydrolytic Stability ◽  
Poly Ethylene Glycol ◽  
Amine Functionalized ◽  
Structures And Properties ◽  
Wide Range ◽  
Poly Ethylene

As a promising strategy for the treatment of various diseases, gene therapy has attracted increasing attention over the past decade. Among various gene delivery approaches, non-viral vectors made of synthetic biomaterials have shown significant potential. Due to their synthetic nature, non-viral vectors can have tunable structures and properties by using various building units. In particular, they can offer advantages over viral vectors with respect to biosafety and cytotoxicity. In this study, a well-defined poly(ethylene glycol)-block-poly(α-(propylthio-N,N-diethylethanamine hydrochloride)-ε-caprolactone) diblock polymer (PEG-b-CPCL) with one poly(ethylene glycol) (PEG) block and one tertiary amine-functionalized cationic poly(ε-caprolactone) (CPCL) block, as a novel non-viral vector in the delivery of plasmid DNA (pDNA), was synthesized and studied. Despite having a degradable polymeric structure, the polymer showed remarkable hydrolytic stability over multiple weeks. The optimal ratio of the polymer to pDNA for nanocomplex formation, pDNA release from the nanocomplex with the presence of heparin, and serum stability of the nanocomplex were probed through gel electrophoresis. Nanostructure of the nanocomplexes was characterized by DLS and TEM imaging. Relative to CPCL homopolymers, PEG-b-CPCL led to better solubility over a wide range of pH. Overall, this work demonstrates that PEG-b-CPCL possesses a range of valuable properties as a promising synthetic vector for pDNA delivery.

scholarly journals Exploitation of the Coil−Globule Plasmid DNA Transition Induced by Small Changes in Temperature, pH Salt, and Poly(ethylene glycol) Compositions for Directed Partitioning in Aqueous Two-Phase Systems

Langmuir ◽  
2006 ◽  
Vol 22 (9) ◽  
pp. 4282-4290 ◽  
Author(s):  
Andreas Frerix ◽  
Michael Schönewald ◽  
Petra Geilenkirchen ◽  
Markus Müller ◽  
Maria-Regina Kula ◽  
...  
Keyword(s):  
Ethylene Glycol ◽  
Plasmid Dna ◽  
Poly Ethylene Glycol ◽  
Two Phase ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
Poly Ethylene
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