A novel method to prepare microcellular poly(vinyl alcohol) foam based on thermal processing and supercritical fluid

2016 ◽  
Vol 28 (3) ◽  
pp. 285-292 ◽  
Author(s):  
Pengju Liu ◽  
Wenhua Chen ◽  
Yingbin Jia ◽  
Shibing Bai
Keyword(s):  
Supercritical Fluid ◽  
Thermal Processing ◽  
Vinyl Alcohol ◽  
Poly Vinyl Alcohol ◽  
Novel Method

A novel method to prepare poly(vinyl alcohol) water-soluble fiber with narrowly dissolving temperature range

2012 ◽  
Vol 125 (4) ◽  
pp. 2956-2962 ◽  
Author(s):  
Bin Wang ◽  
Fan Zhang ◽  
Mengjin Jiang ◽  
Guangdou Ye ◽  
Jianjun Xu
Keyword(s):  
Vinyl Alcohol ◽  
Water Soluble ◽  
Poly Vinyl Alcohol ◽  
Soluble Fiber ◽  
Temperature Range ◽  
Alcohol Water ◽  
Novel Method

scholarly journals Preparation of a Nanoscaled Poly(vinyl alcohol)/Hydroxyapatite/DNA Complex Using High Hydrostatic Pressure Technology for In Vitro and In Vivo Gene Delivery

2011 ◽  
Vol 2011 ◽  
pp. 1-8 ◽  
Author(s):  
Tsuyoshi Kimura ◽  
Yoichi Nibe ◽  
Seiichi Funamoto ◽  
Masahiro Okada ◽  
Tsutomu Furuzono ◽  
...  
Keyword(s):  
Hydrostatic Pressure ◽  
High Hydrostatic Pressure ◽  
Vinyl Alcohol ◽  
Transgene Expression ◽  
Transfection Efficiency ◽  
Endosomal Escape ◽  
Poly Vinyl Alcohol ◽  
Dna Nanoparticles ◽  
Novel Method

Our previous research showed that poly(vinyl alcohol) (PVA) nanoparticles incorporating DNA with hydrogen bonds obtained by high hydrostatic pressurization are able to deliver DNA without any significant cytotoxicity. To enhance transfection efficiency of PVA/DNA nanoparticles, we describe a novel method to prepare PVA/DNA nanoparticles encapsulating nanoscaled hydroxyapatites (HAps) prepared by high hydrostatic pressurization (980 MPa), which is designed to facilitate endosomal escape induced by dissolving HAps in an endosome. Scanning electron microscopic observation and dynamic light scattering measurement revealed that HAps were significantly encapsulated in PVA/HAp/DNA nanoparticles. The cytotoxicity, cellular uptake, and transgene expression of PVA/HAp/DNA nanoparticles were investigated using COS-7 cells. It was found that, in contrast to PVA/DNA nanoparticles, their internalization and transgene expression increased without cytotoxicity occurring. Furthermore, a similar level of transgene expression between plasmid DNA and PVA/HAp/DNA nanoparticles was achieved using in vivo hydrodynamic injection. Our results show a novel method of preparing PVA/DNA nanoparticles encapsulating HAp nano-crystals by using high hydrostatic pressure technology and the potential use of HAps as an enhancer of the transfection efficiency of PVA/DNA nanoparticles without significant cytotoxicity.

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