Galectin-3 is differentially expressed during peripheral nerve development: Dependence on the Schwann cell phenotype

2002 ◽  
Vol 30 (2) ◽  
pp. 71-82 ◽  
Author(s):  
Penka Pesheva ◽  
Joerg Nellen ◽  
Hans-Juergen Biersack ◽  
Ranier Probstmeier
Keyword(s):  
Schwann Cell ◽  
Peripheral Nerve ◽  
Differentially Expressed ◽  
Cell Phenotype ◽  
Galectin 3 ◽  
Nerve Development

Molecular mechanisms in schwann cell survival and death during peripheral nerve development, injury and disease

2005 ◽  
Vol 7 (1-2) ◽  
pp. 151-167 ◽  
Author(s):  
Kristy Boyle ◽  
Michael F. Azari ◽  
Christos Profyris ◽  
Steven Petratos
Keyword(s):  
Schwann Cell ◽  
Peripheral Nerve ◽  
Cell Survival ◽  
Molecular Mechanisms ◽  
Nerve Development

scholarly journals Tissue culture observations relevant to the study of axon-Schwann cell interactions during peripheral nerve development and repair

1987 ◽  
Vol 132 (1) ◽  
pp. 21-34 ◽  
Author(s):  
R. P. Bunge
Keyword(s):  
Tissue Culture ◽  
Schwann Cell ◽  
Peripheral Nerve ◽  
Schwann Cells ◽  
Basal Lamina ◽  
Heparan Sulphate ◽  
Cell Contact ◽  
Myelinated Nerve ◽  
Nerve Development

During peripheral nerve development the Schwann cell population is expanded so that adequate numbers are available for ensheathment of both nonmyelinated and myelinated nerve fibres. As ensheathment of these fibres progresses each axon--Schwann cell unit becomes surrounded by a basal lamina, providing a unique microtubular framework within the peripheral nerve trunk. Tissue culture studies of pure populations of neurones and Schwann cells cultured separately and in combination indicate that a surface component on the axon provides a mitogenic signal to Schwann cells requiring cell-cell contact. Biochemical, electron microscopic and immunocytochemical analyses of these cultures indicate that Schwann cells in contact with axons are able to generate a basal lamina (containing type IV collagen, laminin and heparan sulphate proteoglycan) and fibrous collagen, without the aid of other cells, and that axonal contact is required for deposition of the basal lamina. The role of Schwann cells and the extracellular matrix they synthesize and organize, as well as the role of the other known products of the Schwann cells in the process of peripheral nerve regeneration, are discussed. It is suggested that the large numbers and advantageous position of the Schwann cells, as well as their ability to provide their own surfaces, a basal lamina and multiple secretory products, may account for their extraordinary ability to foster nerve fibre regeneration.

Beta 4 integrin expression in myelinating Schwann cells is polarized, developmentally regulated and axonally dependent

Development ◽  
1994 ◽  
Vol 120 (5) ◽  
pp. 1287-1301 ◽  
Author(s):  
M.L. Feltri ◽  
S.S. Scherer ◽  
R. Nemni ◽  
J. Kamholz ◽  
H. Vogelbacker ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Schwann Cell ◽  
Peripheral Nerve ◽  
Schwann Cells ◽  
Morphological Changes ◽  
Cell Phenotype ◽  
Integrin Subunit ◽  
Integrin Expression ◽  
Cultured Schwann Cells ◽  
Mediate Cell

In developing and regenerating peripheral nerve, Schwann cells interact with axons and extracellular matrix in order to ensheath and myelinate axons. Both of these interactions are likely to be mediated by adhesion molecules, including integrins, which mediate cell-cell and cell-extracellular matrix interactions. Recently, the beta 4 integrin subunit was reported to be expressed by Schwann cells in peripheral nerve. We have examined the expression of beta 4, beta 1 and their common heterodimeric partner, the alpha 6 integrin subunit, in developing and regenerating rat peripheral nerve. beta 4 and alpha 6 are enriched in peripheral nerve and they co-localize at the abaxonal surface of myelinating Schwann cells, opposite the Schwann cell basal lamina, which contains possible ligands of alpha 6 beta 4. In contrast, beta 4 and alpha 6 are expressed in a different pattern in non-myelinating Schwann cells. The level of beta 4, but not alpha 6 or beta 1 mRNAs, increases progressively in developing nerves, reaching a peak in adult nerves well after the peak of the myelin-specific mRNAs. After axotomy, the expression of beta 4 mRNA and protein, but not alpha 6 or beta 1 mRNAs, fall rapidly but subsequently are reinduced by regenerating axons. Similarly, in cultured Schwann cells, the expression of beta 4 mRNA, but not alpha 6 mRNA, is significantly modulated by forskolin, a drug that elevates cAMP and mimics some of the effects of axonal contact. beta 4 integrin expression in Schwann cells, therefore, is regulated by Schwann cell-axon interactions, which are known to be critical in determining the Schwann cell phenotype. Furthermore, the polarized expression of alpha 6 beta 4 to the abaxonal surface of myelinating Schwann cells suggests that alpha 6 beta 4 may mediate in part the morphological changes required of Schwann cells in the process of myelination in the peripheral nervous system.

A co-culture microtunnel technique demonstrating a significant contribution of unmyelinated Schwann cells to the acceleration of axonal conduction in Schwann cell-regulated peripheral nerve development

2017 ◽  
Vol 9 (8) ◽  
pp. 678-686 ◽  
Author(s):  
Koji Sakai ◽  
Kenta Shimba ◽  
Kiyoshi Kotani ◽  
Yasuhiko Jimbo
Keyword(s):  
Schwann Cell ◽  
Peripheral Nerve ◽  
Schwann Cells ◽  
Conduction Velocity ◽  
Significant Contribution ◽  
Culture Technique ◽  
Axonal Conduction ◽  
Nerve Development

The authors developed a co-culture technique inside a microtunnel and demonstrated that Schwann cells increase axonal conduction velocity before myelination.

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