Induction of parathyroid hormone-related peptide following peripheral nerve injury: Role as a modulator of Schwann cell phenotype

Glia ◽  
2006 ◽  
Vol 53 (6) ◽  
pp. 637-648 ◽  
Author(s):  
Carolyn M. Macica ◽  
Guoying Liang ◽  
Karen L. Lankford ◽  
Arthur E. Broadus
Keyword(s):  
Parathyroid Hormone ◽  
Schwann Cell ◽  
Peripheral Nerve ◽  
Nerve Injury ◽  
Peripheral Nerve Injury ◽  
Cell Phenotype ◽  
Related Peptide ◽  
Parathyroid Hormone Related Peptide

LncRNA BC088259 promotes Schwann cell migration through Vimentin following peripheral nerve injury

Glia ◽  
2019 ◽  
Vol 68 (3) ◽  
pp. 670-679 ◽  
Author(s):  
Chun Yao ◽  
Yanping Chen ◽  
Jing Wang ◽  
Tianmei Qian ◽  
Wei Feng ◽  
...  
Keyword(s):  
Cell Migration ◽  
Schwann Cell ◽  
Peripheral Nerve ◽  
Nerve Injury ◽  
Peripheral Nerve Injury ◽  
Schwann Cell Migration

scholarly journals Betacellulin Regulates Peripheral Nerve Regeneration by Affecting Schwann Cell Migration and Axon Elongation

2021 ◽  
Author(s):  
Yaxian Wang ◽  
Fuchao Zhang ◽  
Yunsong Zhang ◽  
Qi Shan ◽  
Wei Liu ◽  
...  
Keyword(s):  
Cell Migration ◽  
Schwann Cell ◽  
Peripheral Nerve ◽  
Schwann Cells ◽  
Nerve Injury ◽  
Peripheral Nerve Injury ◽  
Cultured Cells ◽  
Axon Elongation ◽  
Schwann Cell Migration

Abstract Background Growth factors execute essential biological functions and affect various physiological and pathological processes, including peripheral nerve injury and regeneration. Our previous sequencing analysis found that betacellulin (Btc), an epidermal growth factor protein family member, showed elevated mRNA expressions in the nerve segment after rat peripheral nerve injury, implying the potential involvement of Btc during peripheral nerve repair. Methods Expression of Btc was examined in Schwann cells. The role of Btc in regulating Schwann cells was investigated by transfecting cultured cells with siRNA segment against Btc or exposed cultured cells with Btc recombinant protein, respectively. The biological functions of Schwann cell-secreted Btc on neurons were also determined. Moreover, the in vivo effect of Btc on Schwann cell migration and axon elongation after rat sciatic nerve injury were further evaluated.Results Immunostaining images and ELISA readings showed Btc was present in and secreted by Schwann cells. Transwell migration and wound healing observations showed that siRNA against Btc impeded Schwann cell migration while exogenous Btc advanced Schwann cell migration. Besides the regulating effect on Schwann cell phenotype, Btc secreted by Schwann cells might influence neuron behavior and affect axon length. In vivo evidence showed that Btc enhanced axonal regrowth and nerve regeneration after both rat sciatic nerve crush injury and transection injury. Conclusion Our findings demonstrated Btc-mediated Schwann cell-axon interactions, revealed the essential roles of Btc on Schwann cell migration and axon elongation, and implied the potential application of Btc as a regenerative strategy for treating peripheral nerve injury.

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