RhoA/ROCK and Cdc42 regulate cell-cell contact and N-cadherin protein level during neurodetermination of P19 embryonal stem cells

2004 ◽  
Vol 60 (3) ◽  
pp. 289-307 ◽  
Author(s):  
Isabel Laplante ◽  
Richard Béliveau ◽  
Joanne Paquin
Keyword(s):  
Stem Cells ◽  
Protein Level ◽  
Cell Contact ◽  
Embryonal Stem Cells ◽  
Embryonal Stem ◽  
Cell Cell

scholarly journals Bone marrow mesenchymal stem cells promote prostate cancer cell stemness via cell–cell contact to activate the Jagged1/Notch1 pathway

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Ji-wen Cheng ◽  
Li-xia Duan ◽  
Yang Yu ◽  
Pu Wang ◽  
Jia-le Feng ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Western Blot ◽  
Cell Contact ◽  
Activity Levels ◽  
Tumor Resistance ◽  
Cell Cell

Abstract Background Mesenchymal stem cells (MSCs) play a crucial role in cancer development and tumor resistance to therapy in prostate cancer, but the influence of MSCs on the stemness potential of PCa cells by cell–cell contact remains unclear. In this study, we investigated the effect of direct contact of PCa cells with MSCs on the stemness of PCa and its mechanisms. Methods First, the flow cytometry, colony formation, and sphere formation were performed to determine the stemness of PCaMSCs, and the expression of stemness-related molecules (Sox2, Oct4, and Nanog) was investigated by western blot analysis. Then, we used western blot and qPCR to determine the activity levels of two candidate pathways and their downstream stemness-associated pathway. Finally, we verified the role of the significantly changed pathway by assessing the key factors in this pathway via in vitro and in vivo experiments. Results We established that MSCs promoted the stemness of PCa cells by cell–cell contact. We here established that the enhanced stemness of PCaMSCs was independent of the CCL5/CCR5 pathway. We also found that PCaMSCs up-regulated the expression of Notch signaling-related genes, and inhibition of Jagged1-Notch1 signaling in PCaMSCs cells significantly inhibited MSCs-induced stemness and tumorigenesis in vitro and in vivo. Conclusions Our results reveal a novel interaction between MSCs and PCa cells in promoting tumorigenesis through activation of the Jagged1/Notch1 pathway, providing a new therapeutic target for the treatment of PCa.

Expression of c-myb in embryonal carcinoma cells and embryonal stem cells

1989 ◽  
Vol 42 (1) ◽  
pp. 24-27 ◽  
Author(s):  
P. Julian Dyson ◽  
Francoise Poirier ◽  
Roger J. Watson
Keyword(s):  
Stem Cells ◽  
Embryonal Carcinoma ◽  
Carcinoma Cells ◽  
Embryonal Carcinoma Cells ◽  
Embryonal Stem Cells ◽  
Embryonal Stem

The gene for erythropoietin receptor is expressed in multipotential hematopoietic and embryonal stem cells: evidence for differentiation stage-specific regulation

1992 ◽  
Vol 12 (4) ◽  
pp. 1815-1826
Author(s):  
C Heberlein ◽  
K D Fischer ◽  
M Stoffel ◽  
J Nowock ◽  
A Ford ◽  
...  
Keyword(s):  
Stem Cells ◽  
Hematopoietic Cells ◽  
Cell Surface Receptor ◽  
Erythroid Cell ◽  
Specific Cell ◽  
Erythroid Cells ◽  
Expression Levels ◽  
Embryonal Stem Cells ◽  
First Intron ◽  
Embryonal Stem

The principal regulator of erythropoiesis is the glycoprotein erythropoietin, which interacts with a specific cell surface receptor (EpoR). A study aimed at analyzing EpoR gene regulation has shown that both pluripotent embryonal stem cells and early multipotent hematopoietic cells express EpoR transcripts. Commitment to nonerythroid lineages (e.g., macrophage or lymphocytic) results in the shutdown of EpoR gene expression, whereas commitment to the erythroid lineage is concurrent with or followed by dramatic increases in EpoR transcription. To determine whether gene activity could be correlated with chromatin alterations, DNase-hypersensitive sites (HSS) were mapped. Two major HSS located in the promoter region and within the first intron of the EpoR gene are present in all embryonal stem and hematopoietic cells tested, the intensities of which correlate well with EpoR expression levels. In addition, a third major HSS also located within the first intron of the EpoR gene is uniquely present in erythroid cells that express high levels of EpoR. Transfection assays show that sequences surrounding this major HSS impart erythroid cell-specific enhancer activity to a heterologous promoter and that this activity is at least in part mediated by GATA-1. These data, together with concordant expression levels of GATA-1 and EpoR in both early multipotent hematopoietic and committed erythroid cells, support a regulatory role of the erythroid cell-specific transcription factor GATA-1 in EpoR transcription in these cells. However, the lack of significant levels of GATA-1 expression in embryonal stem cells implies an alternative regulatory mechanism of EpoR transcription in cells not committed to the hematopoietic lineage.

Hematopoietic Differentiation of Embryonal Stem Cells in vitro.

1996 ◽  
pp. 3-10
Author(s):  
Nicholas Hole ◽  
Roland Leung ◽  
Lobat Doostdar ◽  
Ursula Menzel ◽  
Kay Samuel ◽  
...  
Keyword(s):  
Stem Cells ◽  
Hematopoietic Differentiation ◽  
Embryonal Stem Cells ◽  
Embryonal Stem

scholarly journals Oct-6: a POU transcription factor expressed in embryonal stem cells and in the developing brain.

1990 ◽  
Vol 9 (11) ◽  
pp. 3723-3732 ◽  
Author(s):  
N. Suzuki ◽  
H. Rohdewohld ◽  
T. Neuman ◽  
P. Gruss ◽  
H. R. Schöler
Keyword(s):  
Stem Cells ◽  
Transcription Factor ◽  
Developing Brain ◽  
Embryonal Stem Cells ◽  
Embryonal Stem
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