Fast‐Field‐Cycling Nuclear‐Magnetic‐Resonance at very low magnetic fields: water molecular dynamic biomarkers of glioma cell invasion and migration

2021 ◽  
Author(s):  
Manuel Petit ◽  
Maxime Leclercq ◽  
Sandra Pierre ◽  
Maria Rosaria Ruggiero ◽  
Michèle El Atifi ◽  
...  
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Magnetic Fields ◽  
Molecular Dynamic ◽  
Cell Invasion ◽  
Glioma Cell ◽  
Invasion And Migration ◽  
Field Cycling ◽  
And Migration ◽  
Fast Field

Field-cycling NMR experiments in an ultra-wide magnetic field range: relaxation and coherent polarization transfer

2018 ◽  
Vol 20 (18) ◽  
pp. 12396-12405 ◽  
Author(s):  
Ivan V. Zhukov ◽  
Alexey S. Kiryutin ◽  
Alexandra V. Yurkovskaya ◽  
Yuri A. Grishin ◽  
Hans-Martin Vieth ◽  
...  
Keyword(s):  
Magnetic Field ◽  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Magnetic Fields ◽  
Experimental Method ◽  
Field Range ◽  
Magnetic Field Range ◽  
Wide Range ◽  
Field Cycling ◽  
Fast Field

An experimental method is described allowing fast field-cycling Nuclear Magnetic Resonance (NMR) experiments over a wide range of magnetic fields from 5 nT to 10 T.

scholarly journals miRNA-320a inhibits glioma cell invasion and migration by directly targeting aquaporin 4

2018 ◽  
Author(s):  
Wei Xiong ◽  
Jianhua Ran ◽  
Rong Jiang ◽  
Pei Guo ◽  
Xueping Shi ◽  
...  
Keyword(s):  
Cell Invasion ◽  
Glioma Cell ◽  
Aquaporin 4 ◽  
Invasion And Migration ◽  
And Migration

scholarly journals The transcription factor USF1 promotes glioma cell invasion and migration by activating lncRNA HAS2-AS1

2020 ◽  
Vol 40 (8) ◽  
Author(s):  
Juntong Wang ◽  
Jingshun Gu ◽  
Aiwu You ◽  
Jun Li ◽  
Yuyan Zhang ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Cell Invasion ◽  
Promoter Region ◽  
Glioma Cell ◽  
Luciferase Reporter ◽  
Migration And Invasion ◽  
Mesenchymal Transition ◽  
Invasion And Migration ◽  
Starting Point ◽  
And Migration

Abstract Objective: The role of lncRNAs in tumor has been widely concerned. The present study took HAS2-AS1 (the antisense RNA 1 of HAS2) as a starting point to explore its expression in glioma and its role in the process of migration and invasion, providing a strong theoretical basis for mining potential therapeutic targets of glioma. Methods: Clinical data of glioma were obtained from The Cancer Genome Atlas (TCGA) database and differentially expressed lncRNAs were analyzed by edgeR. The hTFtarget database was used to predict the upstream transcription factors of HAS2-AS1 and the JASPAR website was used to predict the binding sites of human upstream transcription factor 1 (USF1) and HAS2-AS1. qRT-PCR was used to detect the expressions of HAS2-AS1 and USF1 in glioma tissues and cell lines. The effects of silencing HAS2-AS1 on the migration and invasion of cancer cells were verified by wound healing and Transwell invasion assays. The chromatin immunoprecipitation (ChIP) and dual luciferase reporter assays were applied to demonstrate the binding of USF1 and HAS2-AS1 promoter region. Western blot was used to detect the expressions of epithelial–mesenchymal transition (EMT)-related proteins. Results: HAS2-AS1 was highly expressed in glioma tissues and cells, and was significantly associated with poor prognosis. Silencing HAS2-AS1 expression inhibited glioma cell migration, invasion and EMT. USF1 was highly expressed in glioma and positively correlated with HAS2-AS1. The transcription of HAS2-AS1 was activated by USF1 via binding to HAS2-AS1 promoter region, consequently potentiating the invasion and migration abilities of glioma cells. Conclusion: These results suggested that the transcription factor USF1 induced up-regulation of lncRNA HAS2-AS1 and promoted glioma cell invasion and migration.

MicroRNAs let-7b/i suppress human glioma cell invasion and migration by targeting IKBKE directly

2015 ◽  
Vol 458 (2) ◽  
pp. 307-312 ◽  
Author(s):  
Yuan Tian ◽  
Shaobo Hao ◽  
Minhua Ye ◽  
Anling Zhang ◽  
Yang Nan ◽  
...  
Keyword(s):  
Cell Invasion ◽  
Glioma Cell ◽  
Human Glioma ◽  
Invasion And Migration ◽  
Human Glioma Cell ◽  
And Migration

scholarly journals Nuclear Magnetic Resonance with Fast Field-Cycling Setup: A Valid Tool for Soil Quality Investigation

Agronomy ◽  
2020 ◽  
Vol 10 (7) ◽  
pp. 1040 ◽  
Author(s):  
Pellegrino Conte ◽  
Paolo Lo Meo
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Nutrient Dynamics ◽  
Relaxation Times ◽  
Liquid Mixtures ◽  
Soil Science ◽  
Nmr Relaxometry ◽  
Field Cycling ◽  
Fast Field ◽  
Nuclear Magnetic

Nuclear magnetic resonance (NMR) techniques are largely employed in several fields. As an example, NMR spectroscopy is used to provide structural and conformational information on pure systems, while affording quantitative evaluation on the number of nuclei in a given chemical environment. When dealing with relaxation, NMR allows understanding of molecular dynamics, i.e., the time evolution of molecular motions. The analysis of relaxation times conducted on complex liquid–liquid and solid–liquid mixtures is directly related to the nature of the interactions among the components of the mixture. In the present review paper, the peculiarities of low resolution fast field-cycling (FFC) NMR relaxometry in soil science are reported. In particular, the general aspects of the typical FFC NMR relaxometry experiment are firstly provided. Afterwards, a discussion on the main mathematical models to be used to “read” and interpret experimental data on soils is given. Following this, an overview on the main results in soil science is supplied. Finally, new FFC NMR-based hypotheses on nutrient dynamics in soils are described

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