In vivo MR tracking of therapeutic microglia to a human glioma model

2011 ◽  
Vol 24 (10) ◽  
pp. 1361-1368 ◽  
Author(s):  
Emeline J. Ribot ◽  
Sylvain Miraux ◽  
Jan P. Konsman ◽  
Véronique Bouchaud ◽  
Line Pourtau ◽  
...  
Keyword(s):  
Human Glioma ◽  
Glioma Model

766 Nuclear Factor κB Blockade by Mutant IkBa Potentiates Chemotherapeutic Efficacy in an in Vivo Human Glioma Model

Neurosurgery ◽  
2001 ◽  
Vol 49 (2) ◽  
pp. 526-526
Author(s):  
Kyle D. Weaver ◽  
Wesley Fowler ◽  
Susan Yeyeodu ◽  
Matthew Ewend
Keyword(s):  
Nuclear Factor Κb ◽  
Nuclear Factor ◽  
Human Glioma ◽  
Chemotherapeutic Efficacy ◽  
Glioma Model

scholarly journals Assessment of multiparametric MRI in a human glioma model to monitor cytotoxic and anti-angiogenic drug effects

2010 ◽  
Vol 24 (5) ◽  
pp. 473-482 ◽  
Author(s):  
Benjamin Lemasson ◽  
Thomas Christen ◽  
Xavier Tizon ◽  
Régine Farion ◽  
Nadège Fondraz ◽  
...  
Keyword(s):  
Drug Effects ◽  
Multiparametric Mri ◽  
Human Glioma ◽  
Glioma Model

Metastasis tumor-associated protein-2 knockdown suppresses the proliferation and invasion of human glioma cells in vitro and in vivo

2014 ◽  
Vol 120 (2) ◽  
pp. 273-281 ◽  
Author(s):  
Chun-Yuan Cheng ◽  
Ying-Erh Chou ◽  
Chung-Po Ko ◽  
Shun-Fa Yang ◽  
Shu-Ching Hsieh ◽  
...  
Keyword(s):  
Glioma Cells ◽  
Human Glioma ◽  
Human Glioma Cells ◽  
Proliferation And Invasion

Invasion of human glioma biopsy specimens in cultures of rodent brain slices: a quantitative analysis

2002 ◽  
Vol 97 (1) ◽  
pp. 169-176 ◽  
Author(s):  
Sophie de Boüard ◽  
Christo Christov ◽  
Jean-Sébastien Guillamo ◽  
Lina Kassar-Duchossoy ◽  
Stéphane Palfi ◽  
...  
Keyword(s):  
Cell Lines ◽  
Brain Slices ◽  
Lower Grade ◽  
Human Glioma ◽  
Newborn Mice ◽  
Content Type ◽  
Biopsy Specimens ◽  
Rodent Brain ◽  
Fine Print

Object. The reliable assessment of the invasiveness of gliomas in vitro has proved elusive, because most invasion assays inadequately model in vivo invasion in its complexity. Recently, organotypical brain cultures were successfully used in short-term invasion studies on glioma cell lines. In this paper the authors report that the invasiveness of human glioma biopsy specimens directly implanted into rodent brain slices by using the intraslice implantation system (ISIS) can be quantified with precision. The model was first validated by the demonstration that, in long-term studies, established glioma cells survive in the ISIS and follow pathways of invasion similar to those in vivo. Methods. Brain slices (400 µm thick) from newborn mice were maintained on millicell membranes for 15 days. Cells from two human and one rodent glioblastoma multiforme (GBM) cell lines injected into the ISIS were detected by immunohistochemistry or after transfection with green fluorescent protein—containing vectors. Preferential migration along blood vessels was identified using confocal and fluorescent microscopy. Freshly isolated (≤ 24 hours after removal) 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate—prelabeled human glioma biopsy specimens were successfully implanted in 19 (83%) of 23 cases, including 12 GBMs and seven lower grade gliomas (LGGs). Morphometric quantification of distance and density of tumor cell invasion showed that the GBMs were two to four times more invasive than the LGGs. Heterogeneity of invasion was also observed among GBMs and LGGs. Directly implanted glioma fragments were more invasive than spheroids derived from the same biopsy specimen. Conclusions. The ISIS combines a high success rate, technical simplicity, and detailed quantitative measurements and may, therefore, be used to study the invasiveness of biopsy specimens of gliomas of different grades.

scholarly journals In vivo detection of c-Met expression in a rat C6 glioma model

2007 ◽  
Vol 12 (1) ◽  
pp. 174-186 ◽  
Author(s):  
R.A. Towner ◽  
N. Smith ◽  
S. Doblas ◽  
Y. Tesiram ◽  
P. Garteiser ◽  
...  
Keyword(s):  
C6 Glioma ◽  
In Vivo Detection ◽  
Glioma Model ◽  
Rat C6 Glioma

scholarly journals Long Intergenic Noncoding RNA 00152 Promotes Glioma Cell Proliferation and Invasion by Interacting with MiR-16

2018 ◽  
Vol 46 (3) ◽  
pp. 1055-1064 ◽  
Author(s):  
Xin Chen ◽  
Deheng Li ◽  
Yang Gao ◽  
Wei Tang ◽  
Lao IW ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Cell Lines ◽  
Glioma Cell ◽  
Migration And Invasion ◽  
Human Glioma ◽  
Human Glioma Cell ◽  
Glioma Cell Lines ◽  
Glioma Cell Proliferation ◽  
Proliferation And Invasion

Background/Aims: Long noncoding RNAs (lncRNAs) are a novel class of protein-noncoding transcripts that are aberrantly expressed in multiple diseases including cancers. LINC00152 has been identified as an oncogene involved in many kinds of cancer; however, its expression pattern and function in human glioma remain unclear. Methods: Quantitative real-time polymerase chain reaction was carried out to measure LINC00152 expression in human glioma cell lines and tissues. CCK-8 and EdU assays were performed to assess cell proliferation, and scratch assays and Transwell assays were used to assess cell migration and invasion, respectively. Luciferase reporter assays were carried out to determine the interaction between miR-16 and LINC00152. In vivo experiments were conducted to assess tumor formation. Results: LINC00152 was found to be significantly upregulated in human glioma cell lines and clinical samples. Knockdown of LINC00152 suppressed glioma cell proliferation, migration, and invasion in vitro. In vivo assays in nude mice confirmed that LINC00152 knockdown inhibits tumor growth. Furthermore, mechanistic investigation showed that LINC00152 binds to miR-16 in a sequence-specific manner and suppresses its expression. miR-16 inhibition strongly attenuated LINC00152 knockdown–mediated suppressive effects on proliferation, migration, and invasion. Moreover, LINC00152 induced BMI1 expression by sponging miR-16; this effect further promoted glioma cell proliferation and invasion. Conclusion: We regard LINC00152 as an oncogenic lncRNA promoting glioma cell proliferation and invasion and as a potential target for human glioma treatment.

scholarly journals Data analyses of honokiol-induced autophagy of human glioma cells in vitro and in vivo

Data in Brief ◽  
2016 ◽  
Vol 9 ◽  
pp. 667-672 ◽  
Author(s):  
Gong-Jhe Wu ◽  
Chien-Ju Lin ◽  
Yung-Wei Lin ◽  
Ruei-Ming Chen
Keyword(s):  
Glioma Cells ◽  
Human Glioma ◽  
Human Glioma Cells ◽  
Data Analyses

Silencing of Hypoxia Inducible Factor-1α by RNA Interference Attenuates Human Glioma Cell Growth In vivo

2007 ◽  
Vol 13 (8) ◽  
pp. 2441-2448 ◽  
Author(s):  
David L. Gillespie ◽  
Kum Whang ◽  
Brian T. Ragel ◽  
Jeannette R. Flynn ◽  
David A. Kelly ◽  
...  
Keyword(s):  
Rna Interference ◽  
Cell Growth ◽  
Glioma Cell ◽  
Hypoxia Inducible Factor ◽  
Human Glioma ◽  
Human Glioma Cell ◽  
Hypoxia Inducible Factor 1Α
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