Assessing manganese efflux using SEA0400 and cardiac T 1 -mapping manganese-enhanced MRI in a murine model

2009 ◽  
Vol 22 (8) ◽  
pp. 874-881 ◽  
Author(s):  
Ben Waghorn ◽  
Yuhui Yang ◽  
Akemichi Baba ◽  
Toshio Matsuda ◽  
Autumn Schumacher ◽  
...  
Keyword(s):  
Murine Model ◽  
Enhanced Mri ◽  
Manganese Enhanced Mri

TU-D-332-04: Modulating Mn2+ Efflux with SEA0400, Using Cardiac Manganese-Enhanced MRI (MEMRI) T1-Mapping in a Murine Model

2008 ◽  
Vol 35 (6Part21) ◽  
pp. 2906-2906
Author(s):  
B Waghorn ◽  
Y Yang ◽  
B Klein ◽  
A Baba ◽  
T Matsuda ◽  
...  
Keyword(s):  
Murine Model ◽  
T1 Mapping ◽  
Enhanced Mri ◽  
Manganese Enhanced Mri

scholarly journals In vivo multi-parametric manganese-enhanced MRI for detecting amyloid plaques in rodent models of Alzheimer’s disease

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Eugene Kim ◽  
Davide Di Censo ◽  
Mattia Baraldo ◽  
Camilla Simmons ◽  
Ilaria Rosa ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Amyloid Plaques ◽  
Transgenic Animals ◽  
Surrounding Tissue ◽  
Rodent Models ◽  
Enhanced Mri ◽  
5Xfad Mice ◽  
Manganese Enhanced Mri

AbstractAmyloid plaques are a hallmark of Alzheimer’s disease (AD) that develop in its earliest stages. Thus, non-invasive detection of these plaques would be invaluable for diagnosis and the development and monitoring of treatments, but this remains a challenge due to their small size. Here, we investigated the utility of manganese-enhanced MRI (MEMRI) for visualizing plaques in transgenic rodent models of AD across two species: 5xFAD mice and TgF344-AD rats. Animals were given subcutaneous injections of MnCl2 and imaged in vivo using a 9.4 T Bruker scanner. MnCl2 improved signal-to-noise ratio but was not necessary to detect plaques in high-resolution images. Plaques were visible in all transgenic animals and no wild-types, and quantitative susceptibility mapping showed that they were more paramagnetic than the surrounding tissue. This, combined with beta-amyloid and iron staining, indicate that plaque MR visibility in both animal models was driven by plaque size and iron load. Longitudinal relaxation rate mapping revealed increased manganese uptake in brain regions of high plaque burden in transgenic animals compared to their wild-type littermates. This was limited to the rhinencephalon in the TgF344-AD rats, while it was most significantly increased in the cortex of the 5xFAD mice. Alizarin Red staining suggests that manganese bound to plaques in 5xFAD mice but not in TgF344-AD rats. Multi-parametric MEMRI is a simple, viable method for detecting amyloid plaques in rodent models of AD. Manganese-induced signal enhancement can enable higher-resolution imaging, which is key to visualizing these small amyloid deposits. We also present the first in vivo evidence of manganese as a potential targeted contrast agent for imaging plaques in the 5xFAD model of AD.

Manganese-enhanced MRI of the mouse auditory pathway

2008 ◽  
Vol 60 (1) ◽  
pp. 210-212 ◽  
Author(s):  
Takashi Watanabe ◽  
Jens Frahm ◽  
Thomas Michaelis
Keyword(s):  
Auditory Pathway ◽  
Enhanced Mri ◽  
Manganese Enhanced Mri

scholarly journals Temporary disruption of the rat blood–brain barrier with a monoclonal antibody: A novel method for dynamic manganese-enhanced MRI

NeuroImage ◽  
2010 ◽  
Vol 50 (1) ◽  
pp. 7-14 ◽  
Author(s):  
Hanbing Lu ◽  
Steven Demny ◽  
Yantao Zuo ◽  
William Rea ◽  
Leiming Wang ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Blood Brain Barrier ◽  
Brain Barrier ◽  
Rat Blood ◽  
Blood Brain ◽  
Enhanced Mri ◽  
Novel Method ◽  
Manganese Enhanced Mri

Reduced hippocampal manganese-enhanced MRI (MEMRI) signal during pilocarpine-induced status epilepticus: Edema or apoptosis?

2014 ◽  
Vol 108 (4) ◽  
pp. 644-652 ◽  
Author(s):  
Jackeline Moraes Malheiros ◽  
Daniele Suzete Persike ◽  
Leticia Urbano Cardoso de Castro ◽  
Talita Rojas Cunha Sanches ◽  
Lúcia da Conceição Andrade ◽  
...  
Keyword(s):  
Status Epilepticus ◽  
Enhanced Mri ◽  
Manganese Enhanced Mri

scholarly journals Manganese-enhanced MRI reveals multiple cellular and vascular layers in normal and degenerated retinas

2011 ◽  
Vol 34 (6) ◽  
pp. 1422-1429 ◽  
Author(s):  
Govind Nair ◽  
Machelle T. Pardue ◽  
Moon Kim ◽  
Timothy Q. Duong
Keyword(s):  
Enhanced Mri ◽  
Manganese Enhanced Mri

Manganese-enhanced MRI (ME MRI) in evaluation of the auditory pathway in an experimental rat model

2016 ◽  
Vol 30 (2) ◽  
pp. e3677 ◽  
Author(s):  
Zuohua Tang ◽  
Lingjie Wu ◽  
Zebin Xiao ◽  
Xiaoyuan Feng ◽  
Xinghuai Sun ◽  
...  
Keyword(s):  
Rat Model ◽  
Auditory Pathway ◽  
Enhanced Mri ◽  
Experimental Rat Model ◽  
Manganese Enhanced Mri

scholarly journals In vivo multi-parametric manganese-enhanced MRI for detecting senile plaques in rodent models of Alzheimer’s disease

2021 ◽  
Author(s):  
Eugene Kim ◽  
Davide Di Censo ◽  
Mattia Baraldo ◽  
Camilla Simmons ◽  
Ilaria Rosa ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
High Resolution ◽  
Senile Plaques ◽  
Rodent Models ◽  
Wild Type ◽  
Enhanced Mri ◽  
Manganese Uptake ◽  
5Xfad Mice ◽  
Manganese Enhanced Mri

AbstractSenile plaques are a hallmark of Alzheimer’s disease (AD) that develop in its earliest stages. Thus, non-invasive detection of these plaques would be invaluable for diagnosis and the development and monitoring of treatments, but this remains a challenge due to their small size. Here, we investigated the utility of manganese-enhanced MRI (MEMRI) for visualizing plaques in transgenic rodent models of AD across two species: 5xFAD mice and TgF344-AD rats.Fourteen mice (eight transgenic, six wild-type) and eight rats (four transgenic, four wild-type) were given subcutaneous injections of MnCl2 and imaged in vivo using a 9.4T Bruker scanner. Susceptibility-weighted images, transverse relaxation rate (R2*) maps, and quantitative susceptibility maps were derived from high-resolution 3D multi-gradient-echo (MGE) data to directly visualize plaques. Longitudinal relaxation rate (R1) maps were derived from MP2RAGE data to measure regional manganese uptake. After scanning, the brains were processed for histology and stained for beta-amyloid (4G8 antibody), iron (Perl’s), and calcium/manganese (Alizarin Red).MnCl2 improved signal-to-noise ratio (1.55±0.39-fold increase in MGE images) as expected, although this was not necessary for detection of plaques in the high-resolution images. Plaques were visible in susceptibility-weighted images, R2* maps, and quantitative susceptibility maps, with increased R2* and more positive magnetic susceptibility compared to surrounding tissue.In the 5xFAD mice, most MR-visible plaques were in the hippocampus, though histology confirmed plaques in the cortex and thalamus as well. In the TgF344-AD rats, many more plaques were MR-visible throughout the hippocampus and cortex. Beta-amyloid and iron staining indicate that, in both models, MR visibility was driven by plaque size and iron load.Voxel-wise comparison of R1 maps revealed increased manganese uptake in brain regions of high plaque burden in transgenic animals compared to their wild-type littermates. Interestingly, in contrast to plaque visibility in the high-resolution images, the increased manganese uptake was limited to the rhinencephalon in the TgF344-AD rats (family-wise error (FWE)-corrected p < 0.05) while it was most significantly increased in the cortex of the 5xFAD mice (FWE-corrected p < 0.3). Alizarin Red staining suggests that manganese bound to plaques in 5xFAD mice but not in TgF344-AD rats.Multi-parametric MEMRI is a simple, viable method for detecting senile plaques in rodent models of AD. Manganese-induced signal enhancement can enable higher-resolution imaging, which is key to visualizing these small amyloid deposits. We also present in vivo evidence of manganese as a potential targeted contrast agent for imaging plaques in the 5xFAD model of AD.HighlightsThis is the first study to use manganese-enhanced MRI (MEMRI) for direct visualization of senile plaques in rodent models of Alzheimer’s disease, in vivo.Manganese enhancement is not necessary to detect plaques but improves image contrast and signal-to-noise ratio.Manganese binds to plaques in 5xFAD mice but not in TgF344-AD rats, demonstrating potential as a targeted contrast agent for imaging plaques in certain models of AD.

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