Murine in vitro whole bladder model: A method for assessing phenotypic responses to pharmacologic stimuli and hypoxia

2004 ◽  
Vol 23 (4) ◽  
pp. 349-354 ◽  
Author(s):  
Joel C. Hutcheson ◽  
Raimund Stein ◽  
Samuel Chacko ◽  
Michael Carr ◽  
Douglas A. Canning ◽  
...  
Keyword(s):  
Phenotypic Responses ◽  
Bladder Model

In Vitro Multicompartmental Bladder Model for Assessing Blockage of Urinary Catheters: Effect of Hydrogel Coating on Dynamics of Proteus mirabilis Growth

Urology ◽  
2010 ◽  
Vol 76 (2) ◽  
pp. 515.e15-515.e20 ◽  
Author(s):  
Katarzyna A. Kazmierska ◽  
Richard Thompson ◽  
Nicola Morris ◽  
Adele Long ◽  
Tomasz Ciach
Keyword(s):  
Proteus Mirabilis ◽  
Urinary Catheters ◽  
Hydrogel Coating ◽  
Bladder Model

Development of an in vitro bladder model

1992 ◽  
Vol 7 (4) ◽  
pp. 35-37
Author(s):  
Anne Mulhall
Keyword(s):  
Bladder Model

Ascarids exposed: a method for in vitro drug exposure and gene expression analysis of anthelmintic naïve Parascaris spp

Parasitology ◽  
2020 ◽  
Vol 147 (6) ◽  
pp. 659-666 ◽  
Author(s):  
J. A. Scare ◽  
P. Dini ◽  
J. K. Norris ◽  
A. E. Steuer ◽  
K. Scoggin ◽  
...  
Keyword(s):  
Drug Exposure ◽  
Anthelmintic Resistance ◽  
Resistance Mechanisms ◽  
Transcriptomic Analysis ◽  
Rna Seq ◽  
Future Studies ◽  
Drug Concentrations ◽  
Drug Detoxification ◽  
Phenotypic Responses

AbstractAscarid parasites infect a variety of hosts and regular anthelmintic treatment is recommended for all species. Parascaris spp. is the only ascarid species with widespread anthelmintic resistance, which allows for the study of resistance mechanisms. The purpose of this study was to establish an in vitro drug exposure protocol for adult anthelmintic-naïve Parascaris spp. and report a preliminary transcriptomic analysis in response to drug exposure. Live worms were harvested from foal necropsies and maintained in RPMI-1640 at 37 °C. Serial dilutions of oxibendazole (OBZ) and ivermectin (IVM) were prepared for in vitro drug exposure, and worm viability was monitored over time. In a second drug trial, worms were used for transcriptomic analysis. The final drug concentrations employed were OBZ at 40.1 μm (10 μg mL−1) and IVM at 1.1 μm (1 μg mL−1) for 24 and 3 h, respectively. The RNA-seq analysis revealed numerous differentially expressed genes, with some being potentially related to drug detoxification and regulatory mechanisms. This report provides a method for in vitro drug exposure and the phenotypic responses for Parascaris spp., which could be extrapolated to other ascarid parasites. Finally, it also provides preliminary transcriptomic data following drug exposure as a reference point for future studies of Parascaris spp.

An In Vitro Study of a New Device: Evaluation of Novel Wide-Angle Lens Flexible Cystoscope Using Phantom Bladder Model

2017 ◽  
Vol 31 (10) ◽  
pp. 1073-1078
Author(s):  
Kunihisa Yamaguchi ◽  
Takamitsu Inoue ◽  
Tomonori Habuchi ◽  
Junichi Inokuchi ◽  
Akira Yokomizo ◽  
...  
Keyword(s):  
In Vitro Study ◽  
Vitro Study ◽  
Wide Angle ◽  
New Device ◽  
Flexible Cystoscope ◽  
Wide Angle Lens ◽  
Bladder Model

scholarly journals Demonstration of the direct impact of ketamine on urothelium using a tissue engineered bladder model

2015 ◽  
Vol 9 (9-10) ◽  
pp. 613 ◽  
Author(s):  
Michel Bureau ◽  
Jérôme Pelletier ◽  
Alexandre Rousseau ◽  
Geneviève Bernard ◽  
Stéphane Chabaud ◽  
...  
Keyword(s):  
Human Tissue ◽  
Specific Activity ◽  
Three Dimensional ◽  
Fold Increase ◽  
Dermal Fibroblasts ◽  
Population Exposure ◽  
Urothelial Cells ◽  
Three Dimensional Model ◽  
Bladder Model

Introduction: Ketamine is a common recreational drug. Severe lower urinary tract symptoms associated with its consumption have been reported, but little is known about the involved mechanisms. The effect of ketamine, which is excreted in urine, was evaluated by its application on an in vitro three-dimensional human tissue-engineered bladder model composed of an urothelium and a submucosa.Methods: Human urothelial cells were cultured with medium containing various concentrations of ketamine and harvested at different times to obtain growth curves. Using this model, specific activity of caspase-3 was measured to assess the level of apoptosis induced by ketamine. Finally, a human tissue-engineered bladder model was used. Urothelial cells were plated on a stromal layer made of dermal fibroblasts and incubated at the air/liquid interface to allow their differentiation. Ketamine was then put on the mature urothelium using paper or agarose vectors for 48 hours.Results: The presence of ketamine increased cells’ doubling times from 1.26 days for control to 1.38 days (p = 0.14) and 1.78 days (p < 0.01) for the 0.5 mM and 1.5 mM concentrations, respectively. 5 mM and 10 mM of ketamine led to decline in the major cell population. Exposure to 5 mM ketamine induced apoptosis, confirmed by a 2.5-fold increase in capase-3 specific activity from control (p = 0.03). The structure and cellular cohesion of the urothelium on the three-dimensional model, especially in the intermediate layers, were severely affected in a concentration dependant fashion with both vectors.Conclusion: The presence of ketamine in the bladder directly damages the urothelium through the induction of apoptosis.

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