Mast cells can regulate skeletal muscle cell proliferation by multiple mechanisms

2013 ◽  
Vol 48 (3) ◽  
pp. 403-414 ◽  
Author(s):  
Elise Duchesne ◽  
Patrice Bouchard ◽  
Marie-Pier Roussel ◽  
Claude H. Côté
Keyword(s):  
Skeletal Muscle ◽  
Cell Proliferation ◽  
Mast Cells ◽  
Muscle Cell ◽  
Skeletal Muscle Cell

scholarly journals Phytochemicals in Garlic Extract Inhibit Therapeutic Enzyme DPP-4 and Induce Skeletal Muscle Cell Proliferation: A Possible Mechanism of Action to Benefit the Treatment of Diabetes Mellitus

Biomolecules ◽  
2020 ◽  
Vol 10 (2) ◽  
pp. 305
Author(s):  
Poonam Kalhotra ◽  
Veera C.S.R. Chittepu ◽  
Guillermo Osorio-Revilla ◽  
Tzayhri Gallardo-Velazquez
Keyword(s):  
Diabetes Mellitus ◽  
Mass Spectrometry ◽  
Skeletal Muscle ◽  
Cell Proliferation ◽  
Muscle Cell ◽  
Mechanism Of Action ◽  
Skeletal Muscle Cell ◽  
Garlic Extract ◽  
Garlic Bulb ◽  
Treatment Of Diabetes

Diabetes mellitus is a severe health problem in Mexico, and its prevalence is increasing exponentially every year. Recently, DPP-4 (dipeptidyl peptidase-4) inhibitors have become attractive oral anti-hyperglycemic agents to reduce the pathology of diabetes. Gliptin’s family, such as sitagliptin, vildagliptin, and alogliptin, are in clinical use to treat diabetes mellitus but possess side effects. Therefore, there is a specific need to look for new therapeutic scaffolds (biomolecules). Garlic bulb is widely used as a traditional remedy for the treatment of diabetes. The garlic extracts are scientifically proven to control glucose levels in patients with diabetes, despite the unknown mechanism of action. The aim of the study is to investigate the antidiabetic effects of ultrasonication assisted garlic bulb extract. To achieve this, in-vitro assays such as DPP-4 inhibitory and antioxidant activities were investigated. Further, functional group analysis using FTIR and identification of phytochemicals using mass spectrometry analysis was performed. The results showed that 70.9 µg/mL of garlic bulb extract inhibited 50% DPP-4 activity. On top of that, the garlic extract exhibited a 20% scavenging activity, equivalent to 10 µg/mL of ascorbic acid. Molecular docking simulations on identified phytochemicals using mass spectrometry revealed their potential binding at the DPP-4 druggable region, and therefore the possible DPP-4 inhibition mechanism. These results suggest that prepared garlic extract contains phytochemicals that inhibit DPP-4 and have antioxidant activity. Also, the prepared extract induces skeletal muscle cell proliferation that demonstrates the antidiabetic effect and its possible mechanism of action.

scholarly journals Mast Cells Appearing in Long-term Skeletal Muscle Cell Cultures of Rat

2007 ◽  
Vol 290 (11) ◽  
pp. 1424-1430 ◽  
Author(s):  
Chang-xie Cheng ◽  
Yong-nan Li ◽  
Hiroshi Ohno ◽  
Kazuko Sawanobori ◽  
Yan-chao Li ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Mast Cells ◽  
Muscle Cell ◽  
Cell Cultures ◽  
Skeletal Muscle Cell

miR-34b Modulates Skeletal Muscle Cell Proliferation and Differentiation

2017 ◽  
Vol 118 (12) ◽  
pp. 4285-4295 ◽  
Author(s):  
Zhixiong Tang ◽  
Huiling Qiu ◽  
Lan Luo ◽  
Nian Liu ◽  
Jiasheng Zhong ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Cell Proliferation ◽  
Muscle Cell ◽  
Skeletal Muscle Cell ◽  
Cell Proliferation And Differentiation ◽  
Proliferation And Differentiation

scholarly journals Designed Functional Dispersion for Insulin Protection from Pepsin Degradation and Skeletal Muscle Cell Proliferation: In Silico and In Vitro Study

Nanomaterials ◽  
2018 ◽  
Vol 8 (10) ◽  
pp. 852 ◽  
Author(s):  
Veera Chittepu ◽  
Poonam Kalhotra ◽  
Tzayhri Gallardo-Velázquez ◽  
Raúl Robles-de la Torre ◽  
Guillermo Osorio-Revilla
Keyword(s):  
Skeletal Muscle ◽  
Cell Proliferation ◽  
Insulin Therapy ◽  
Muscle Cell ◽  
Carbon Nanomaterials ◽  
Pharmaceutical Preparations ◽  
In Vitro Study ◽  
Skeletal Muscle Cell ◽  
Functional Dispersion

Functionalized single-walled carbon nanotubes with polyethylene glycol (PEGylated SWCNTs) are a promising nanomaterial that recently has emerged as the most attractive “cargo” to deliver chemicals, peptides, DNA and RNAs into cells. Insulin therapy is a recommended therapy to treat diabetes mellitus despite its side effects. Recently, functional dispersion made up of bioactive peptides, bioactive compounds and functionalized carbon nanomaterials such as PEGylated SWCNTs have proved to possess promising applications in nanomedicine. In the present study, molecular modeling simulations are utilized to assist in designing insulin hormone-PEGylated SWCNT composites, also called functional dispersion; to achieve this experimentally, an ultrasonication tool was utilized. Enzymatic degradation assay revealed that the designed functional dispersion protects about 70% of free insulin from pepsin. In addition, sulforhodamine B (SRB) assay, the quantification of insulin and glucose levels in differentiated skeletal muscle cell supernatants, reveals that functional dispersion regulates glucose and insulin levels to promote skeletal muscle cell proliferation. These findings offer new perspectives for designed functional dispersion, as potential pharmaceutical preparations to improve insulin therapy and promote skeletal muscle cell health.

scholarly journals Phytochemicals in Chinese chive (Allium tuberosum) Induce the Skeletal Muscle Cell Proliferation via PI3K/Akt/mTOR and Smad Pathways in C2C12 Cells

2021 ◽  
Vol 22 (5) ◽  
pp. 2296
Author(s):  
Mira Oh ◽  
Seo-Young Kim ◽  
SeonJu Park ◽  
Kil-Nam Kim ◽  
Seung Hyun Kim
Keyword(s):  
Skeletal Muscle ◽  
Cell Proliferation ◽  
Muscle Cell ◽  
Muscle Growth ◽  
Skeletal Muscle Cell ◽  
Water Soluble ◽  
C2c12 Cells ◽  
Allium Tuberosum ◽  
Skeletal Muscle Growth ◽  
Chinese Chive

Chinese chive (Allium tuberosum) is a medicinal food that is cultivated and consumed mainly in Asian countries. Its various phytochemicals and physiological effects have been reported, but only a few phytochemicals are available for skeletal muscle cell proliferation. Herein, we isolated a new compound, kaempferol-3-O-(6″-feruloyl)-sophoroside (1), along with one known flavonoid glycoside (2) and six amino acid (3–8) compounds from the water-soluble fraction of the shoot of the Chinese chive. The isolated compounds were identified using extensive spectroscopic methods, including 1D and 2D NMR, and evaluated for their proliferation activity on skeletal muscle cells. Among the tested compounds, newly isolated flavonoid (1) and 5-aminouridine (7) up-regulated PI3K/Akt/mTOR pathways, which implies a positive effect on skeletal muscle growth and differentiation. In particular, compound 1 down-regulated the Smad pathways, which are negative regulators of skeletal muscle growth. Collectively, we suggest that major constituents of Chinese chive, flavonoids and amino acids, might be used in dietary supplements that aid skeletal muscle growth.

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