scholarly journals Mast Cells Appearing in Long-term Skeletal Muscle Cell Cultures of Rat

2007 ◽  
Vol 290 (11) ◽  
pp. 1424-1430 ◽  
Author(s):  
Chang-xie Cheng ◽  
Yong-nan Li ◽  
Hiroshi Ohno ◽  
Kazuko Sawanobori ◽  
Yan-chao Li ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Mast Cells ◽  
Muscle Cell ◽  
Cell Cultures ◽  
Skeletal Muscle Cell

Specific effect of corticoids on acetylcholine receptor expression in rat skeletal muscle cell cultures

1992 ◽  
Vol 31 (2) ◽  
pp. 285-293 ◽  
Author(s):  
J.-T. Vilquin ◽  
S. Braun ◽  
P. Labouret ◽  
G. Zuber ◽  
C. Tranchant ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Acetylcholine Receptor ◽  
Muscle Cell ◽  
Cell Cultures ◽  
Specific Effect ◽  
Skeletal Muscle Cell ◽  
Receptor Expression ◽  
Rat Skeletal Muscle

scholarly journals Ca 2+ effects on glucose transport and fatty acid oxidation in L6 skeletal muscle cell cultures

2016 ◽  
Vol 5 ◽  
pp. 365-373 ◽  
Author(s):  
Darrick Balu ◽  
Jiangyong Ouyang ◽  
Rahulkumar A. Parakhia ◽  
Saumitra Pitake ◽  
Raymond S. Ochs
Keyword(s):  
Skeletal Muscle ◽  
Fatty Acid ◽  
Glucose Transport ◽  
Fatty Acid Oxidation ◽  
Muscle Cell ◽  
Cell Cultures ◽  
Skeletal Muscle Cell ◽  
Acid Oxidation ◽  
L6 Skeletal Muscle Cell

scholarly journals Pharmacological activation of AMPK and glucose uptake in cultured human skeletal muscle cells from patients with ME/CFS

2018 ◽  
Vol 38 (3) ◽  
Author(s):  
Audrey E. Brown ◽  
Beth Dibnah ◽  
Emily Fisher ◽  
Julia L. Newton ◽  
Mark Walker
Keyword(s):  
Skeletal Muscle ◽  
Glucose Uptake ◽  
Muscle Cell ◽  
Cell Cultures ◽  
Human Skeletal Muscle ◽  
Muscle Cells ◽  
Skeletal Muscle Cell ◽  
Ampk Activation ◽  
Atp Content ◽  
Human Skeletal Muscle Cells

Skeletal muscle fatigue and post-exertional malaise are key symptoms of myalgic encephalomyelitis (ME)/chronic fatigue syndrome (ME/CFS). We have previously shown that AMP-activated protein kinase (AMPK) activation and glucose uptake are impaired in primary human skeletal muscle cell cultures derived from patients with ME/CFS in response to electrical pulse stimulation (EPS), a method which induces contraction of muscle cells in vitro. The aim of the present study was to assess if AMPK could be activated pharmacologically in ME/CFS. Primary skeletal muscle cell cultures from patients with ME/CFS and healthy controls were treated with either metformin or compound 991. AMPK activation was assessed by Western blot and glucose uptake measured. Both metformin and 991 treatment significantly increased AMPK activation and glucose uptake in muscle cell cultures from both controls and ME/CFS. Cellular ATP content was unaffected by treatment although ATP content was significantly decreased in ME/CFS compared with controls. Pharmacological activation of AMPK can improve glucose uptake in muscle cell cultures from patients with ME/CFS. This suggests that the failure of EPS to activate AMPK in these muscle cultures is due to a defect proximal to AMPK. Further work is required to delineate the defect and determine whether pharmacological activation of AMPK improves muscle function in patients with ME/CFS.

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