7-Nitroindazole potentiates c-fos expression induced by muscle tendon vibration in the spinal cord

2012 ◽  
Vol 45 (4) ◽  
pp. 597-602 ◽  
Author(s):  
Alexander I. Pilyavskii ◽  
Vladimir A. Maisky ◽  
Andrey V. Maznychenko ◽  
Alexander I. Kostyukov
Keyword(s):  
Spinal Cord ◽  
Tendon Vibration ◽  
Muscle Tendon Vibration ◽  
Fos Expression

scholarly journals On the reflex mechanisms of cervical transcutaneous spinal cord stimulation in human subjects

2019 ◽  
Vol 121 (5) ◽  
pp. 1672-1679 ◽  
Author(s):  
Matija Milosevic ◽  
Yohei Masugi ◽  
Atsushi Sasaki ◽  
Dimitry G. Sayenko ◽  
Kimitaka Nakazawa
Keyword(s):  
Spinal Cord ◽  
Muscle Contraction ◽  
Upper Limb ◽  
Spinal Cord Stimulation ◽  
Presynaptic Inhibition ◽  
Tendon Vibration ◽  
Muscle Stretching ◽  
Muscle Tendon Vibration ◽  
Passive Muscle ◽  
Transcutaneous Spinal Cord Stimulation

Transcutaneous and epidural electrical spinal cord stimulation techniques are becoming more valuable as electrophysiological and clinical tools. Recently, remarkable recovery of the upper limb sensorimotor function during cervical spinal stimulation was demonstrated. In the present study, we sought to elucidate the neural mechanisms underlying the effects of transcutaneous spinal cord stimulation (tSCS) of the cervical spine. We hypothesized that cervical tSCS can be used to selectively activate the sensory route entering the spinal cord and transsynaptically converge on upper limb motor pools. To test this hypothesis, we applied cervical tSCS using paired stimuli (homosynaptic depression) and during passive muscle stretching of the wrist flexor (presynaptic inhibition via Ia afferents), voluntary hand muscle contraction (descending facilitation of motoneuron pool), and muscle-tendon vibration of the wrist (presynaptic inhibition via afferent occlusion). Our results demonstrate significant inhibition of the second evoked response during paired stimulus delivery, inhibition of responses during passive muscle stretching and muscle-tendon vibration, and facilitation during voluntary muscle contraction, which share similarities with responses evoked during lumbosacral tSCS. These results indicate that the route of the stimulation current transmission passes via afferents in the dorsal roots through the spinal cord to activate the motor pools and potentially interneuronal networks projecting to upper limb muscles. Using a novel stimulation paradigm, our study is the first to present evidence of the sensory neuronal pathway of the cervical tSCS propagation. Overall, our work demonstrates the utility and sensitivity of cervical tSCS to engage the sensory pathway projecting to the upper limbs. NEW & NOTEWORTHY Despite therapeutic effects that have been demonstrated previously using noninvasive cervical spinal stimulation, it has been unclear whether, and to what degree, the stimulation can activate the sensory afferent system. Our study presents evidence that cervical transcutaneous spinal cord stimulation can engage the sensory pathways and transsynaptically converge on motor pools projecting to upper limb muscles, demonstrating the utility and sensitivity of cervical spinal stimulation for electrophysiological assessments and neurorehabilitation.

The effect of stimulus duration on noxious-stimulus induced c-fos expression in the rodent spinal cord

1992 ◽  
Vol 580 (1-2) ◽  
pp. 172-179 ◽  
Author(s):  
Elizabeth Bullitt ◽  
Chong Lam Lee ◽  
Alan R. Light ◽  
Helen Willcockson
Keyword(s):  
Spinal Cord ◽  
Stimulus Duration ◽  
Noxious Stimulus ◽  
Fos Expression

Carrageenin-evoked c-Fos expression in rat lumbar spinal cord: the effects of indomethacin

1995 ◽  
Vol 272 (2-3) ◽  
pp. 249-259 ◽  
Author(s):  
Prisca Honoré ◽  
Jaroslava Buritova ◽  
Jean-Marie Besson
Keyword(s):  
Spinal Cord ◽  
Lumbar Spinal Cord ◽  
Fos Expression ◽  
Lumbar Spinal

c-Fos expression in rat spinal cord induced by scorpion BmK venom via plantar subcutaneous injection

2002 ◽  
Vol 44 (4) ◽  
pp. 447-454 ◽  
Author(s):  
Zhan-Tao Bai ◽  
Bing Chen ◽  
Xu-Ying Zhang ◽  
Guang-Li Fan ◽  
Yong-Hua Ji
Keyword(s):  
Spinal Cord ◽  
Subcutaneous Injection ◽  
Rat Spinal Cord ◽  
Fos Expression

c-Fos expression in the spinal cord after acute sacral segmental nerve stimulation

2002 ◽  
Vol 21 (5) ◽  
pp. 495-501 ◽  
Author(s):  
Manabu Ishigooka ◽  
Teruhiro Nakada ◽  
Tohru Hashimoto ◽  
Dirk-Henrik Zermann ◽  
Richard A. Schmidt
Keyword(s):  
Spinal Cord ◽  
Nerve Stimulation ◽  
Segmental Nerve ◽  
Fos Expression

The Effect of a Thoracic Spinal Block on Fos Expression in the Lumbar Spinal Cord of the Rat Induced by a Noxious Electrical Stimulus at the Hindpaw

2009 ◽  
Vol 109 (5) ◽  
pp. 1659-1665
Author(s):  
Janneke L. P. Giele ◽  
Anneke F. Nabers ◽  
Jan G. Veening ◽  
Jan van Egmond ◽  
Kris C. P. Vissers
Keyword(s):  
Spinal Cord ◽  
Electrical Stimulus ◽  
Lumbar Spinal Cord ◽  
Spinal Block ◽  
Thoracic Spinal ◽  
Fos Expression ◽  
Lumbar Spinal

Role of NMDA and AMPA glutamatergic transmission in spinal c-fos expression after urinary tract irritation

1996 ◽  
Vol 270 (5) ◽  
pp. R990-R996 ◽  
Author(s):  
H. Kakizaki ◽  
M. Yoshiyama ◽  
W. C. de Groat
Keyword(s):  
Spinal Cord ◽  
Urinary Tract ◽  
Nmda Antagonist ◽  
Early Gene ◽  
Mk 801 ◽  
Sacral Parasympathetic Nucleus ◽  
Fos Expression ◽  
Dose Dependent Decrease ◽  
Nociceptive Input

Chemical irritation of the lower urinary tract (LUT) of the rat increases the expression of the immediate early gene c-fos within neurons in the dorsal horn (DH), dorsal commissure (DCM), and intermediolateral region, including sacral parasympathetic nucleus (SPN) of the spinal cord (L6-S1). A previous study indicated the involvement of the N-methyl-D-aspartic acid (NMDA) receptor in this c-fos expression after LUT irritation. The role of glutamatergic synapses was further investigated using a selective and competitive alpha-amino-3-hydroxy-5-methylisoxazole-4-propionic acid (AMPA) receptor antagonist (LY-215490). Systemic administration of LY-215490 produced a dose-dependent decrease in the number of Fos-positive cells after LUT irritation in the DCM and SPN areas, whereas in the DH only the highest dose (10 mg/kg) of LY-215490 decreased the number of Fos-positive cells. A low dose (1 mg/kg) of either MK-801 (an NMDA antagonist) or LY-215490 alone did not alter c-fos expression. However, a combined administration of low doses of MK-801 and LY-215490 significantly decreased the number of Fos-positive cells in all regions of the spinal cord. These results indicate that AMPA as well as NMDA receptors are involved in the spinal processing of nociceptive input from the LUT and that these glutamatergic receptors play a synergistic role in visceral nociceptive processing.

Concomitant Administration of Morphine and an N-Methyl-D-Aspartate Receptor Antagonist Profoundly Reduces Inflammatory Evoked Spinal c-Fos Expression

1996 ◽  
Vol 85 (1) ◽  
pp. 150-160 ◽  
Author(s):  
Prisca D. Honore ◽  
Victoria Chapman ◽  
Jaroslava Buritova ◽  
Jean-Marie Besson
Keyword(s):  
Spinal Cord ◽  
Concomitant Administration ◽  
Receptor Activation ◽  
Intraplantar Injection ◽  
Receptor Antagonism ◽  
Mu Opioid ◽  
Nociceptive Transmission ◽  
Aspartate Receptor ◽  
Fos Expression ◽  
Two Parameters

Background After intraplantar injection of carrageenin, peripheral inflammation and spinal c-Fos expression are extensive, with the latter being sensitive to both large doses of morphine or N-methyl-D-aspartate receptor antagonism. The authors investigated the effects of coadministered morphine and (+)-HA966, a functional antagonist at the glycine site of the N-methyl-D-aspartate receptor, on the two parameters. Methods The effects of morphine, (+)-HA966 and coadministration of morphine and (+)-HA966 on spinal c-Fos expression in segments L4-L5 of the spinal cord and peripheral edema, induced at 1.5 h and 3 h after intraplantar carrageenin (6 mg/150 microliters) were studied. Results Previous coadministration of 0.3 mg/kg systemic morphine and 2.5 mg/kg subcutaneous (+)-HA966 significantly reduced c-Fos expression induced 1.5 h, but not 3 h, after carrageenin administration. However, coadministration of a larger dose of morphine (3 mg/kg) with (+)-HA966 (2.5 mg/kg) reduced c-Fos expression at 3 h after carrageenin administration, in a partially naloxone-reversible manner. Conclusions Concurrent mu-opioid receptor activation and N-methyl-D-aspartate receptor antagonism reduces nociceptive transmission at the level of the spinal cord, as shown by the reduction of carrageenin-evoked c-Fos expression.

Elimination of rat spinal neurons expressing neurokinin 1 receptors reduces bladder overactivity and spinal c-fos expression induced by bladder irritation

2005 ◽  
Vol 288 (3) ◽  
pp. F466-F473 ◽  
Author(s):  
Satoshi Seki ◽  
Kristin A. Erickson ◽  
Masako Seki ◽  
Osamu Nishizawa ◽  
Yasuhiko Igawa ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Dorsal Horn ◽  
Intrathecal Administration ◽  
Superficial Layer ◽  
Sprague Dawley ◽  
Intrathecal Catheter ◽  
Micturition Reflex ◽  
Nociceptive Responses ◽  
Fos Expression ◽  
Neurokinin 1

Substance P (SP) binding to neurokinin 1 receptors (NK1R) in the spinal cord reportedly plays an important role in the micturition reflex as well as in nociceptive responses. We therefore investigated the effect of ablation of NK1R-expressing neurons in the spinal cord using saporin, a ribosome-inactivating protein, conjugated with [Sar9, Met (O2)11]SP, a specific ligand of NK1R (SSP-saporin), on the micturition reflex in rats. In female Sprague-Dawley rats, SSP-saporin (1.0 or 1.5 μM) or saporin (1.5 μM) only was injected through an intrathecal catheter implanted at the L6-S1 level of the spinal cord. Three weeks after intrathecal administration of SSP-saporin, NK1R immunoreactivity in lamina I of the spinal cord was significantly reduced, but cystometric parameters in awake rats were not altered. Instillation of capsaicin (15 μM) into the bladder of normal rats induced bladder overactivity. This response to capsaicin was significantly suppressed in SSP-saporin-treated animals. SSP-saporin treatment also decreased c- fos expression in the dorsal horn of the spinal cord induced by instillation of capsaicin into the bladder. These data indicate that NK1R-expressing neurons in the superficial layer of the dorsal horn play an important role in transmission of nociceptive afferent information from the bladder to induce bladder overactivity and spinal c- fos expression elicited by bladder irritation. Toxin-induced damage of NK1R-expressing neurons in the lumbosacral spinal cord may provide an effective modality for treating overactivity and/or nociceptive responses in the bladder without affecting normal micturition.

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