Synchrotron X‐ray micro‐CT as a validation dataset for diffusion MRI in whole mouse brain

2021 ◽  
Author(s):  
Scott Trinkle ◽  
Sean Foxley ◽  
Narayanan Kasthuri ◽  
Patrick La Rivière
Keyword(s):  
Mouse Brain ◽  
Diffusion Mri ◽  
Validation Dataset ◽  
Micro Ct ◽  
X Ray

scholarly journals In situ dynamic X-ray micro-CT for additive manufactured parts

2021 ◽  
Vol 27 (S1) ◽  
pp. 2944-2945
Author(s):  
Jan Dewanckele ◽  
Frederik Coppens ◽  
Wesley De Boever ◽  
Marijn Boone ◽  
Luke Hunter
Keyword(s):  
Micro Ct ◽  
X Ray

scholarly journals Nanostructure-specific X-ray tomography reveals myelin levels, integrity and axon orientations in mouse and human nervous tissue

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Marios Georgiadis ◽  
Aileen Schroeter ◽  
Zirui Gao ◽  
Manuel Guizar-Sicairos ◽  
Marianne Liebi ◽  
...  
Keyword(s):  
Mouse Brain ◽  
Nervous Tissue ◽  
Signal Transmission ◽  
X Ray ◽  
Tensor Tomography ◽  
Quantitative Studies ◽  
X Ray Scattering ◽  
Imaging Approach ◽  
Non Destructive ◽  
Mri Measurements

AbstractMyelin insulates neuronal axons and enables fast signal transmission, constituting a key component of brain development, aging and disease. Yet, myelin-specific imaging of macroscopic samples remains a challenge. Here, we exploit myelin’s nanostructural periodicity, and use small-angle X-ray scattering tensor tomography (SAXS-TT) to simultaneously quantify myelin levels, nanostructural integrity and axon orientations in nervous tissue. Proof-of-principle is demonstrated in whole mouse brain, mouse spinal cord and human white and gray matter samples. Outcomes are validated by 2D/3D histology and compared to MRI measurements sensitive to myelin and axon orientations. Specificity to nanostructure is exemplified by concomitantly imaging different myelin types with distinct periodicities. Finally, we illustrate the method’s sensitivity towards myelin-related diseases by quantifying myelin alterations in dysmyelinated mouse brain. This non-destructive, stain-free molecular imaging approach enables quantitative studies of myelination within and across samples during development, aging, disease and treatment, and is applicable to other ordered biomolecules or nanostructures.

scholarly journals Simple low dose radiography allows precise lung volume assessment in mice

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Amara Khan ◽  
Andrea Markus ◽  
Thomas Rittmann ◽  
Jonas Albers ◽  
Frauke Alves ◽  
...  
Keyword(s):  
Lung Function ◽  
Whole Body ◽  
Acquisition Time ◽  
Micro Ct ◽  
Lung Volumes ◽  
X Ray ◽  
Promising Tool ◽  
Volume Assessment ◽  
Set Up ◽  
Time Required

AbstractX-ray based lung function (XLF) as a planar method uses dramatically less X-ray dose than computed tomography (CT) but so far lacked the ability to relate its parameters to pulmonary air volume. The purpose of this study was to calibrate the functional constituents of XLF that are biomedically decipherable and directly comparable to that of micro-CT and whole-body plethysmography (WBP). Here, we developed a unique set-up for simultaneous assessment of lung function and volume using XLF, micro-CT and WBP on healthy mice. Our results reveal a strong correlation of lung volumes obtained from radiographic XLF and micro-CT and demonstrate that XLF is superior to WBP in sensitivity and precision to assess lung volumes. Importantly, XLF measurement uses only a fraction of the radiation dose and acquisition time required for CT. Therefore, the redefined XLF approach is a promising tool for preclinical longitudinal studies with a substantial potential of clinical translation.

scholarly journals Dynamic X-ray micro-CT insights of the recovery of ore bodies in presence of clay

2021 ◽  
Vol 27 (S1) ◽  
pp. 1034-1035
Author(s):  
Jan Dewanckele ◽  
Wesley De Boever ◽  
Andreas Grießer ◽  
Yanhong Wang ◽  
Fangli Meng
Keyword(s):  
Micro Ct ◽  
X Ray ◽  
Ore Bodies

X-ray micro-CT with a displaced detector array: Application to helical cone-beam reconstruction

2003 ◽  
Vol 30 (10) ◽  
pp. 2758-2761 ◽  
Author(s):  
Vinson Liu ◽  
Nicholas R. Lariviere ◽  
Ge Wang
Keyword(s):  
Detector Array ◽  
Cone Beam ◽  
Micro Ct ◽  
X Ray ◽  
Cone Beam Reconstruction

Automated segmentation of murine lung tumors in x-ray micro-CT images

2014 ◽  
Author(s):  
Joshua K. Y. Swee ◽  
Clare Sheridan ◽  
Elza de Bruin ◽  
Julian Downward ◽  
Francois Lassailly ◽  
...  
Keyword(s):  
Lung Tumors ◽  
Ct Images ◽  
Automated Segmentation ◽  
Micro Ct ◽  
Murine Lung ◽  
X Ray

scholarly journals Probing mouse brain microstructure using oscillating gradient diffusion MRI

2011 ◽  
Vol 67 (1) ◽  
pp. 98-109 ◽  
Author(s):  
Manisha Aggarwal ◽  
Melina V. Jones ◽  
Peter A. Calabresi ◽  
Susumu Mori ◽  
Jiangyang Zhang
Keyword(s):  
Mouse Brain ◽  
Diffusion Mri ◽  
Gradient Diffusion ◽  
Brain Microstructure

scholarly journals A LabVIEW Platform for Preclinical Imaging Using Digital Subtraction Angiography and Micro-CT

2013 ◽  
Vol 2013 ◽  
pp. 1-13 ◽  
Author(s):  
Cristian T. Badea ◽  
Laurence W. Hedlund ◽  
G. Allan Johnson
Keyword(s):  
Digital Subtraction Angiography ◽  
Functional Imaging ◽  
Imaging System ◽  
Micro Ct ◽  
Digital Subtraction ◽  
X Ray ◽  
Retrospective Gating ◽  
Dual Source ◽  
Labview Platform ◽  
And Control

CT and digital subtraction angiography (DSA) are ubiquitous in the clinic. Their preclinical equivalents are valuable imaging methods for studying disease models and treatment. We have developed a dual source/detector X-ray imaging system that we have used for both micro-CT and DSA studies in rodents. The control of such a complex imaging system requires substantial software development for which we use the graphical language LabVIEW (National Instruments, Austin, TX, USA). This paper focuses on a LabVIEW platform that we have developed to enable anatomical and functional imaging with micro-CT and DSA. Our LabVIEW applications integrate and control all the elements of our system including a dual source/detector X-ray system, a mechanical ventilator, a physiological monitor, and a power microinjector for the vascular delivery of X-ray contrast agents. Various applications allow cardiac- and respiratory-gated acquisitions for both DSA and micro-CT studies. Our results illustrate the application of DSA for cardiopulmonary studies and vascular imaging of the liver and coronary arteries. We also show how DSA can be used for functional imaging of the kidney. Finally, the power of 4D micro-CT imaging using both prospective and retrospective gating is shown for cardiac imaging.

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