scholarly journals Serial in vivo positive contrast MRI of iron oxide-labeled embryonic stem cell-derived cardiac precursor cells in a mouse model of myocardial infarction

2008 ◽  
Vol 60 (1) ◽  
pp. 73-81 ◽  
Author(s):  
Venkatesh Mani ◽  
Eric Adler ◽  
Karen C. Briley-Saebo ◽  
Anne Bystrup ◽  
Valentin Fuster ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Stem Cell ◽  
Iron Oxide ◽  
Mouse Model ◽  
Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Positive Contrast ◽  
Precursor Cells ◽  
Cardiac Precursor Cells

scholarly journals Developmental Changes of Ni2+ Sensitivity and Automaticity in Nkx2.5-Positive Cardiac Precursor Cells From Murine Embryonic Stem Cell

2004 ◽  
Vol 68 (7) ◽  
pp. 724-726 ◽  
Author(s):  
Kasumi Manabe ◽  
Junichiro Miake ◽  
Norihito Sasaki ◽  
Hitomi Furuichi ◽  
Shuichi Yano ◽  
...  
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Precursor Cells ◽  
Developmental Changes ◽  
Murine Embryonic Stem Cell ◽  
Cardiac Precursor Cells

scholarly journals ROCK suppression promotes differentiation and expansion of endothelial cells from embryonic stem cell–derived Flk1+ mesodermal precursor cells

Blood ◽  
2012 ◽  
Vol 120 (13) ◽  
pp. 2733-2744 ◽  
Author(s):  
Hyung Joon Joo ◽  
Dong-Kyu Choi ◽  
Joon Seo Lim ◽  
Jin-Sung Park ◽  
Seung-Hun Lee ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Precursor Cells ◽  
Rock Inhibitor ◽  
Downstream Signaling ◽  
Matrigel Culture

Abstract Successful differentiation and expansion of endothelial cells (ECs) from embryonic stem cell (ESC)–derived Flk1+ mesodermal precursor cells (MPCs) requires supplementation of vascular endothelial growth factor-A (VEGF-A). While analyzing VEGF-A/VEGFR2 downstream signaling pathway that underlies the VEGF-A-induced differentiation and expansion of ECs, we fortuitously found that Rho-associated protein kinase (ROCK) inhibitor Y27632 profoundly promoted the differentiation and expansion of ECs from Flk1+ MPCs while reducing the differentiation and expansion of mural cells. The ROCK suppression-induced expansion of ECs appears to have resulted from promotion of proliferation of ECs via activation of PI3-kinase-Akt signaling. The ECs obtained by the combination of ROCK suppression and VEGF-A supplementation faithfully expressed most pan-EC surface makers, and phenotypic analyses revealed that they were differentiated toward arterial EC. Further incubation of the ICAM2+ ECs with Y27632 and VEGF-A for 2 days promoted expansion of ECs by 6.5-fold compared with those incubated with only VEGF-A. Importantly, the ROCK suppression-induced ECs displayed neovasculogenic abilities in vitro and in vivo. Thus, supplementation of ROCK inhibitor Y27632 along with VEGF-A in 2D Matrigel culture system provides a simple, efficient, and versatile method for obtaining ample amount of ESC-derived ECs at high purity suitable for use in therapeutic neovascularization.

scholarly journals In vivo gene delivery by embryonic-stem-cell–derived astrocytes for malignant gliomas

2009 ◽  
Vol 11 (2) ◽  
pp. 102-108 ◽  
Author(s):  
Mahmud Uzzaman ◽  
Gordon Keller ◽  
Isabelle M. Germano
Keyword(s):  
Stem Cell ◽  
Gene Delivery ◽  
Embryonic Stem Cell ◽  
Malignant Gliomas ◽  
Embryonic Stem ◽  
In Vivo Gene Delivery

scholarly journals Human embryonic stem cell-derived microvascular grafts for cardiac tissue preservation after myocardial infarction

Biomaterials ◽  
2011 ◽  
Vol 32 (4) ◽  
pp. 1102-1109 ◽  
Author(s):  
Thomas P. Kraehenbuehl ◽  
Lino S. Ferreira ◽  
Alison M. Hayward ◽  
Matthias Nahrendorf ◽  
André J. van der Vlies ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Human Embryonic Stem Cell ◽  
Cardiac Tissue ◽  
Embryonic Stem ◽  
Tissue Preservation

scholarly journals In vitro and in vivo analyses of human embryonic stem cell-derived dopamine neurons

2005 ◽  
Vol 92 (5) ◽  
pp. 1265-1276 ◽  
Author(s):  
Chang-Hwan Park ◽  
Yang-Ki Minn ◽  
Ji-Yeon Lee ◽  
Dong Ho Choi ◽  
Mi-Yoon Chang ◽  
...  
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Human Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Dopamine Neurons

scholarly journals Znanstvena istraživanja u 21. stoljeću - etički aspekti uporabe laboratorijskih životinja i alternativni modeli

2022 ◽  
Vol 53 (5) ◽  
Author(s):  
Ivana Kmetič ◽  
Monika Roller ◽  
Marina Miletić ◽  
Teuta Murati
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Embryo Culture ◽  
Embryonic Stem ◽  
Whole Embryo Culture ◽  
Cell Test

U toksikološkim istraživanjima uz uporabu klasičnih (in vivo) istraživanja, primjenjuju se alternativni test sustavi. Korištenje laboratorijskih životinja, embrija, humanog i animalnog tkiva, kultura stanica i fetalnog seruma u istraživanjima smatra se etički problematičnim te se ograničava zakonima, pravilnicima i praksom. Razmatranjem načina kojima bi se neetičnost mogla izbjeći, došlo je do razvoja “3R” načela (akronim za tri pristupa koja bi se trebala provoditi pri istraživanjima na laboratorijskim životinjama), a to su: smanjenje/racionalizacija uporabe laboratorijskih životinja (engl. Reduction), načelo njihove zamjene (engl. Replacement) i poboljšanje uvjeta uzgoja, smještaja i skrbi za životinje (engl. Refinement). Većina je alternativnih testova toksičnosti još uvijek u postupku validacije. Pojedini in vitro testovi za istraživanja embriotoksičnosti (etički posebno osjetljivo područje) koja su priznala nadležna regulatorna tijela, su EST (engl. Embryonic Stem cell Test), WEC (engl. Whole- Embryo Culture) i MM (engl. MicroMass) test. Standardizacija protokola i uvođenje novih in vitro modela predstavlja važan segment napretka u toksikološkim istraživanjima. Znanstvena budućnost tu vidi mogućnost razvoja i implementacije načela etičnosti u istraživanja primjenjujući sustave koji će promišljeno i bez korištenja živih organizama dijelom nadomjestiti metode u biomedicini, veterinarskoj medicini, biotehnologiji i užem smislu - toksikologiji i farmakologiji.

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