scholarly journals 13C saturation transfer effect of carbon dioxide–bicarbonate exchange catalyzed by carbonic anhydrase in vivo

2008 ◽  
Vol 59 (3) ◽  
pp. 492-498 ◽  
Author(s):  
Jehoon Yang ◽  
Sujata Singh ◽  
Jun Shen
Keyword(s):  
Carbon Dioxide ◽  
Carbonic Anhydrase ◽  
Transfer Effect ◽  
Saturation Transfer

scholarly journals Why Is Carbonic Anhydrase Essential to Escherichia coli?

2003 ◽  
Vol 185 (21) ◽  
pp. 6415-6424 ◽  
Author(s):  
Christophe Merlin ◽  
Millicent Masters ◽  
Sean McAteer ◽  
Andrew Coulson
Keyword(s):  
Escherichia Coli ◽  
Carbon Dioxide ◽  
Carbonic Anhydrase ◽  
Carbon Dioxide Concentration ◽  
Growth Cycle ◽  
Anaerobic Growth ◽  
Methanosarcina Thermophila ◽  
E Coli ◽  
Expression Studies

ABSTRACT The can (previously yadF) gene of Escherichia coli encodes a β-class carbonic anhydrase (CA), an enzyme which interconverts CO2 and bicarbonate.Various essential metabolic processes require either CO2 or bicarbonate and, although carbon dioxide and bicarbonate spontaneously equilibrate in solution, the low concentration of CO2 in air and its rapid diffusion from the cell mean that insufficient bicarbonate is spontaneously made in vivo to meet metabolic and biosynthetic needs. We calculate that demand for bicarbonate is 103- to 104-fold greater than would be provided by uncatalyzed intracellular hydration and that enzymatic conversion of CO2 to bicarbonate is therefore necessary for growth. We find that can expression is ordinarily required for growth in air. It is dispensable if the atmospheric partial pressure of CO2 is high or during anaerobic growth in a closed vessel at low pH, where copious CO2 is generated endogenously. CynT, the single E. coli Can paralog, can, when induced with azide, replace Can; also, the γ-CA from Methanosarcina thermophila can at least partially replace it. Expression studies showed that can transcription does not appear to respond to carbon dioxide concentration or to be autoregulated. However, can expression is influenced by growth rate and the growth cycle; it is expressed best in slow-growing cultures and at higher culture densities. Expression can vary over a 10-fold range during the growth cycle and is also elevated during starvation or heat stress.

Carbonic anhydrase activity in leaves as measured in vivo by 18O exchange between carbon dioxide and water

Planta ◽  
1995 ◽  
Vol 196 (4) ◽  
Author(s):  
Gilles Peltier ◽  
Laurent Cournac ◽  
Val�rie Despax ◽  
Bernard Dimon ◽  
Laurent Fina ◽  
...  
Keyword(s):  
Carbon Dioxide ◽  
Carbonic Anhydrase ◽  
Carbonic Anhydrase Activity

Carbonic anhydrase activity in leaves as measured in vivo by18O exchange between carbon dioxide and water

Planta ◽  
1995 ◽  
Vol 196 (4) ◽  
pp. 732-739 ◽  
Author(s):  
Gilles Peltier ◽  
Laurent Cournac ◽  
Val�rie Despax ◽  
Bernard Dimon ◽  
Laurent Fina ◽  
...  
Keyword(s):  
Carbon Dioxide ◽  
Carbonic Anhydrase ◽  
Carbonic Anhydrase Activity

scholarly journals Protective effects of carbonic anhydrase inhibition in brain ischaemia in vitro and in vivo models

2021 ◽  
Vol 36 (1) ◽  
pp. 964-976
Author(s):  
Ilaria Dettori ◽  
Irene Fusco ◽  
Irene Bulli ◽  
Lisa Gaviano ◽  
Elisabetta Coppi ◽  
...  
Keyword(s):  
Carbonic Anhydrase ◽  
Protective Effects ◽  
Brain Ischaemia ◽  
In Vivo Models ◽  
Carbonic Anhydrase Inhibition
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