In vivo magnetic resonance imaging of single cells in mouse brain with optical validation

2005 ◽  
Vol 55 (1) ◽  
pp. 23-29 ◽  
Author(s):  
Chris Heyn ◽  
John A. Ronald ◽  
Lisa T. Mackenzie ◽  
Ian C. MacDonald ◽  
Ann F. Chambers ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Magnetic Resonance ◽  
Mouse Brain ◽  
Single Cells ◽  
Resonance Imaging

scholarly journals Neuroanatomy of the fragile X knockout mouse brain studied using in vivo high resolution magnetic resonance imaging

1999 ◽  
Vol 7 (5) ◽  
pp. 526-532 ◽  
Author(s):  
R Frank Kooy ◽  
Edwin Reyniers ◽  
Marleen Verhoye ◽  
Jan Sijbers ◽  
Cathy E Bakker ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Magnetic Resonance ◽  
High Resolution ◽  
Mouse Brain ◽  
Knockout Mouse ◽  
Fragile X ◽  
Resonance Imaging

scholarly journals Highly efficient endosomal labeling of progenitor and stem cells with large magnetic particles allows magnetic resonance imaging of single cells

Blood ◽  
2003 ◽  
Vol 102 (3) ◽  
pp. 867-872 ◽  
Author(s):  
Kathleen A. Hinds ◽  
Jonathan M. Hill ◽  
Erik M. Shapiro ◽  
Mikko O. Laukkanen ◽  
Alfonso C. Silva ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Iron Oxide ◽  
Magnetic Resonance ◽  
Magnetic Particles ◽  
Single Cells ◽  
Resonance Imaging ◽  
Cd34 Cells

Abstract Tracking transplanted stem cells using magnetic resonance imaging (MRI) could offer biologic insight into homing and engraftment. Ultrasmall dextran-coated iron oxide particles have previously been developed for uptake into cells to allow MRI tracking. We describe a new application of much larger, micron-scale, iron oxide magnetic particles with enhanced MR susceptibility, which enables detection of single cells at resolutions that can be achieved in vivo. In addition, these larger particles possess a fluorophore for histologic confirmation of cell distribution. We demonstrate highly efficient, nontoxic, endosomal uptake of these particles into hematopoietic CD34+ cells and mesenchymal stem cells documented by confocal and electron microscopy. Labeled cells retain biologic activity with preservation of colony-forming ability and differentiation capacity. MRI studies could detect labeled CD34+ cells and mesenchymal stem cells (MSCs) at single cell resolution. This appears to be a promising tool for serial noninvasive monitoring of in vivo cell homing and localization using MRI.

In vivo diffusion tensor magnetic resonance imaging and fiber tracking of the mouse brain

2010 ◽  
Vol 23 (7) ◽  
pp. 884-896 ◽  
Author(s):  
Laura-Adela Harsan ◽  
Dominik Paul ◽  
Susanne Schnell ◽  
Bjorn W. Kreher ◽  
Jürgen Hennig ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Magnetic Resonance ◽  
Mouse Brain ◽  
Diffusion Tensor ◽  
Fiber Tracking ◽  
Resonance Imaging

Monitoring stem cell migration in the nervous system by in vivo magnetic resonance imaging

2005 ◽  
Vol 25 (1_suppl) ◽  
pp. S692-S692
Author(s):  
Mathias Hoehn ◽  
Uwe Himmelreich ◽  
Ralph Weber ◽  
Pedro Ramos-Cabrer ◽  
Susanne Wegener ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Nervous System ◽  
Stem Cell ◽  
Cell Migration ◽  
Magnetic Resonance ◽  
Resonance Imaging ◽  
Stem Cell Migration

Supramolecular Enhancement of Aminoxyl ORCA Contrast Agents

2019 ◽  
Author(s):  
Hamilton Lee ◽  
Jenica Lumata ◽  
Michael A. Luzuriaga ◽  
Candace Benjamin ◽  
Olivia Brohlin ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Side Effects ◽  
Magnetic Resonance ◽  
Tobacco Mosaic Virus ◽  
Contrast Agents ◽  
Single Molecule ◽  
Organic Radical ◽  
Resonance Imaging ◽  
Physiological Conditions

<div><div><div><p>Many contrast agents for magnetic resonance imaging are based on gadolinium, however side effects limit their use in some patients. Organic radical contrast agents (ORCAs) are potential alternatives, but are reduced rapidly in physiological conditions and have low relaxivities as single molecule contrast agents. Herein, we use a supramolecular strategy where cucurbit[8]uril binds with nanomolar affinities to ORCAs and protects them against biological reductants to create a stable radical in vivo. We further over came the weak contrast by conjugating this complex on the surface of a self-assembled biomacromolecule derived from the tobacco mosaic virus.</p></div></div></div>

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