scholarly journals India ink: A potential clinically applicable EPR oximetry probe

1994 ◽  
Vol 31 (2) ◽  
pp. 229-232 ◽  
Author(s):  
Harold M. Swartz ◽  
Ke Jian Liu ◽  
Fuminori Goda ◽  
Tadeusz Walczak
1995 ◽  
Vol 34 (6) ◽  
pp. 888-892 ◽  
Author(s):  
Toshiaki Nakashima ◽  
Fuminori Goda ◽  
Jinge Jiang ◽  
Toshihide Shima ◽  
Harold M. Swartz
Keyword(s):  

Author(s):  
Benjamin B. Williams ◽  
Nadeem Khan ◽  
Bassem Zaki ◽  
Alan Hartford ◽  
Marc S. Ernstoff ◽  
...  

2001 ◽  
Vol 30 (6) ◽  
pp. 568-569 ◽  
Author(s):  
Ayyakkannu Manivannan ◽  
Hisao Yanagi

1996 ◽  
Vol 80 (2) ◽  
pp. 552-558 ◽  
Author(s):  
J. Jiang ◽  
T. Nakashima ◽  
K. J. Liu ◽  
F. Goda ◽  
T. Shima ◽  
...  

With the use of India ink and lithium phthalocyanine (LiPc) as electron paramagnetic resonance (EPR) oximetry probes, the PO2 of the liver was investigated in mice. Because India ink was taken up by the Kupffer cells of the liver, the EPR signal of the India ink reflected the average PO2 in phagocytic vesicles of these cells. The mean value of PO2 in the Kupffer cells measured by India ink was 15.3 +/- 4.4 Torr in unanesthetized animals. LiPc was administered as a macroscopic crystal and therefore reflected the PO2 of the overall liver. The PO2 measured by LiPc was 23.4 +/- 4.4 Torr, which is consistent with the median value of 23.5 Torr reported previously with the use of an oxygen electrode. Anesthesia (injection of 60 mg/kg ip pentobarbital sodium) decreased the average PO2 in both Kupffer cells and the overall liver. The effects of perturbing the blood flow were studied by reversible blockage of the portal vein and hepatic artery in anesthetized mice; the PO2 in both Kupffer cells (measured with India ink) and in overall liver (measured with LiPc) decreased with obstruction of the blood flow and returned toward normal with reperfusion. These results indicate that the PO2 in different locations in the liver can be significantly different under both normal conditions and after perturbation by either anesthesia or ischemia. These results also demonstrate that EPR oximetry can provide sensitive measurements of PO2 in the liver in vivo under various conditions.


Author(s):  
F.E. Hossler ◽  
M.I. McKamey ◽  
F.C. Monson

A comprehensive study of the microvasculature of the normal rabbit bladder, revealed unusual "capillary glomeruli" along the lateral walls. Here they are characterized as hemal lymph nodes using light microscopy, SEM, TEM, ink injection, and vascular casting.Bladders were perfused via a cannula placed in the abdominal aorta with either 2% glutaraldehyde in 0.1M cacodylate buffer (pH 7.4) for fixation, 10% India ink in 0.9% saline and 0.1M phosphate (pH 7.4) for vessel tracing, or resin (Mercoximethylmethacrylate: catalyst, 4:1:0.3; Ladd Research Industries) for vascular corrosion casting. Infusion pressure was 100mm Hg. Fixed tissue was sectioned from epon-araldyte resin, and stained with toluidine blue for light microscopy, and lead and uranium for TEM. Ink injected tissue was photographed directly from saline-filled bladders illuminated from below. Resin-filled tissue was macerated in 5% KOH and distilled water. Casts were critical point dried, sputter coated with goldpalladium, and examined by routine SEM at 10 KV.


Swiss Surgery ◽  
2003 ◽  
Vol 9 (6) ◽  
pp. 307-310 ◽  
Author(s):  
Gianom ◽  
Hollinger ◽  
Wirth

Die laparoskopische Kolonchirurgie benötigt oft als unterstützende Massnahme eine präoperative Markierung der Läsion, da dem Chirurgen die intraoperative orientierende Palpation fehlt. Die endoskopische Markierung mit Tusche ist aufgrund der Einfachheit und der langanhaltenden Markierung die Methode der Wahl. Nur wenige Komplikationen sind bei dieser Technik beschrieben. In unserem Fall traten im Anschluss an die Markierung einer idiopathischen ulzerösen Kolonveränderung progrediente Bauchschmerzen auf. Bei der Operation fand sich eine retroperitoneale gedeckte Perforation. Die entzündlichen Veränderungen waren derart, dass ein laparoskopisches Vorgehen unmöglich war und eine offene Hemikolektomie rechts notwendig war. Bei Fieber, Abdominalschmerzen und Zeichen der lokalen Peritonitis im Anschluss an eine endoskopische Tuschmarkierung muss an eine unerwünschte Reaktion auf die Tusche gedacht werden.


1969 ◽  
Vol 22 (03) ◽  
pp. 508-512
Author(s):  
L Pechet ◽  
Giselle S. Pechet ◽  
R. A MacDonald

SummaryIntravascular coagulation and its possible effect on carbon clearance was studied in rats following the injection of commercial india ink containing shellac; a shellac-free carbon preparation; gelatin; heat denatured albumin; colloidal iron; and heparin. No relationship was found between activation of coagulation and RES function as measured by clearance of intravenously injected carbon.


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