Identification of amniotic fluid metabolomic and placental transcriptomic changes associated with abnormal development of cloned pig fetuses

2019 ◽  
Vol 86 (3) ◽  
pp. 278-291 ◽  
Author(s):  
Zheng Ao ◽  
Zicong Li ◽  
Xingwang Wang ◽  
Chengfa Zhao ◽  
Yanmin Gan ◽  
...  
Keyword(s):  
Amniotic Fluid ◽  
Abnormal Development ◽  
Transcriptomic Changes ◽  
Cloned Pig

scholarly journals RNA Sequencing Reveals Diverse Functions of Amniotic Fluid Neutrophils and Monocytes/Macrophages in Intra-Amniotic Infection

2020 ◽  
pp. 1-20
Author(s):  
Nardhy Gomez-Lopez ◽  
Roberto Romero ◽  
Aneesha Varrey ◽  
Yaozhu Leng ◽  
Derek Miller ◽  
...  
Keyword(s):  
Amniotic Fluid ◽  
Immune Cells ◽  
Protective Role ◽  
Innate Immune ◽  
Innate Immune Cells ◽  
Amniotic Cavity ◽  
Fetal Origin ◽  
Term Delivery ◽  
Adverse Pregnancy ◽  
Transcriptomic Changes

Intra-amniotic infection, the invasion of microbes into the amniotic cavity resulting in inflammation, is a clinical condition that can lead to adverse pregnancy outcomes for the mother and fetus as well as severe long-term neonatal morbidities. Despite much research focused on the consequences of intra-amniotic infection, there remains little knowledge about the innate immune cells that respond to invading microbes. We performed RNA-seq of sorted amniotic fluid neutrophils and monocytes/macrophages from women with intra-amniotic infection to determine the transcriptomic differences between these innate immune cells. Further, we sought to identify specific transcriptomic pathways that were significantly altered by the maternal or fetal origin of amniotic fluid neutrophils and monocytes/macrophages, the presence of a severe fetal inflammatory response, and pregnancy outcome (i.e., preterm or term delivery). We show that significant transcriptomic differences exist between amniotic fluid neutrophils and monocytes/macrophages from women with intra-amniotic infection, indicating the distinct roles these cells play. The transcriptome of amniotic fluid immune cells varies based on their maternal or fetal origin, and the significant transcriptomic differences between fetal and maternal monocytes/macrophages imply that those of fetal origin exhibit impaired functions. Notably, transcriptomic changes in amniotic fluid monocytes/macrophages suggest that these immune cells collaborate with neutrophils in the trafficking of fetal leukocytes throughout the umbilical cord (i.e., funisitis). Finally, amniotic fluid neutrophils and monocytes/macrophages from preterm deliveries display enhanced transcriptional activity compared to those from term deliveries, highlighting the protective role of these cells during this vulnerable period. Collectively, these findings demonstrate the underlying complexity of local innate immune responses in women with intra-amniotic infection and provide new insights into the functions of neutrophils and monocytes/macrophages in the amniotic cavity.

scholarly journals The Amniotic Fluid Cell-Free Transcriptome Provides Novel Information about Fetal Development and Placental Cellular Dynamics

2021 ◽  
Vol 22 (5) ◽  
pp. 2612
Author(s):  
Hee Jin Park ◽  
Hee Young Cho ◽  
Dong Hyun Cha
Keyword(s):  
Amniotic Fluid ◽  
Maternal Obesity ◽  
Fetal Lung ◽  
Well Being ◽  
Abnormal Development ◽  
Amniotic Fluid Cell ◽  
Genetic Syndromes ◽  
Placental Development ◽  
Organ Systems ◽  
Cell Free Fetal Dna

The amniotic fluid (AF) is a complex biofluid that reflects fetal well-being during development. AF con be divided into two fractions, the supernatant and amniocytes. The supernatant contains cell-free components, including placenta-derived microparticles, protein, cell-free fetal DNA, and cell-free fetal RNA from the fetus. Cell-free mRNA (cfRNA) analysis holds a special position among high-throughput analyses, such as transcriptomics, proteomics, and metabolomics, owing to its ease of profiling. The AF cell-free transcriptome differs from the amniocyte transcriptome and alters with the progression of pregnancy and is often associated with the development of various organ systems including the fetal lung, skin, brain, pancreas, adrenal gland, gastrointestinal system, etc. The AF cell-free transcriptome is affected not only by normal physiologies, such as fetal sex, gestational age, and fetal maturity, but also by pathologic mechanisms such as maternal obesity, and genetic syndromes (Down, Edward, Turner, etc.), as well as pregnancy complications (preeclampsia, intrauterine growth restriction, preterm birth, etc.). cfRNA in the amniotic fluid originates from the placenta and fetal organs directly contacting the amniotic fluid as well as from the fetal plasma across the placenta. The AF transcriptome may reflect the fetal and placental development and therefore aid in the monitoring of normal and abnormal development.

Significance of meconium stained amniotic fluid in the preterm population

1998 ◽  
Vol 5 (1) ◽  
pp. 167A-167A
Author(s):  
H SCOTT ◽  
M WALKER ◽  
L OPPENHEIMER ◽  
A GRUSLINGIROUX
Keyword(s):  
Amniotic Fluid ◽  
Meconium Stained Amniotic Fluid
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