A novel approach to identify bovine sperm membrane proteins that interact with receptors on the vitelline membrane of bovine oocytes

2008 ◽  
Vol 75 (4) ◽  
pp. 641-649 ◽  
Author(s):  
Barry J. Pate ◽  
Kenneth L. White ◽  
Dong Chen ◽  
Kenneth I. Aston ◽  
Benjamin R. Sessions ◽  
...  
Keyword(s):  
Membrane Proteins ◽  
Vitelline Membrane ◽  
Bovine Sperm ◽  
Novel Approach ◽  
Sperm Membrane ◽  
Bovine Oocytes

PURIFICATION OF BOVINE SPERM MEMBRANE-PROTEINS INVOLVED IN SPERM-OOCYTE INTERACTIONS: ADHESION, FUSION, AND ACTIVATION

2007 ◽  
Vol 77 (Suppl_1) ◽  
pp. 88-88
Author(s):  
Ammon Bayles ◽  
Benjamin Sessions ◽  
Kenneth Aston ◽  
John Brinkerhoff ◽  
Dong Chen ◽  
...  
Keyword(s):  
Membrane Proteins ◽  
Bovine Sperm ◽  
Sperm Membrane

Structure, Expression, and Hormonal Control of Genes from the Mosquito, Aedes aegypti, Which Encode Proteins Similar to the Vitelline Membrane Proteins of Drosophila melanogaster

1993 ◽  
Vol 155 (2) ◽  
pp. 558-568 ◽  
Author(s):  
Yonggu Lin ◽  
Martha T. Hamblin ◽  
Marten J. Edwards ◽  
Carolina Barillas-Mury ◽  
Michael R. Kanost ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Membrane Proteins ◽  
Aedes Aegypti ◽  
Hormonal Control ◽  
Vitelline Membrane

Activation of bovine oocytes matured in vitro by injection of bovine and human spermatozoa or their cytosolic fractions

Zygote ◽  
2001 ◽  
Vol 9 (1) ◽  
pp. 89-95 ◽  
Author(s):  
Osamu Okitsu ◽  
Shuji Yamano ◽  
Toshihiro Aono
Keyword(s):  
Human Sperm ◽  
Human Spermatozoa ◽  
Oocyte Activation ◽  
Sham Injection ◽  
Bovine Sperm ◽  
Female Pronucleus ◽  
Sperm Factor ◽  
Bovine Spermatozoa ◽  
Bovine Oocytes

The aim of this study was to investigate whether bovine spermatozoa possess so-called sperm factor in the cytosolic fraction (CF) which activates bovine oocytes, and whether bovine oocytes matured in vitro are activated by microinjection of CF extracted from spermatozoa of other species. In the first experiment, bovine and human spermatozoa were microinjected into ooplasm of bovine oocytes matured in vitro. Secondly, CF from bovine and human spermatozoa were injected into bovine oocytes. In the third, CF from human spermatozoa was injected into human unfertilised oocytes obtained 18-20 h after clinical intracytoplasmic sperm injection (ICSI). We found that microinjection of bovine spermatozoa into bovine oocytes induced oocyte activation, as shown by resumption of meiosis and formation of a female pronucleus, at a significantly higher rate than the bovine sham injection (63.0% vs 43.0%; p < 0.05). On the other hand, there was no significant difference in activation rate between the human sperm injection (35.9%) and the human sham injection (22.9%). Furthermore, microinjection of bovine sperm CF into bovine oocytes induced oocyte activation at a significantly higher rate than the human CF injection or sham injection (75.9% vs 14.8%, 20.4%; p < 0.01). Formation of a single female pronucleus and second polar body extrusion was observed in 95.1% of activated oocytes after bovine sperm CF injection. When human sperm CF was injected into human unfertilised oocytes, the activation rate was significantly higher than following sham injection (76.9% vs 44.0%; p < 0.05). These results indicate the presence of sperm factor in bovine sperm CF which activate bovine oocytes, and suggest the possibility that sperm factor has species-specificity at least between bovine and human.

Analysis of vitelline membrane proteins of fresh and stored eggs via HPLC

1998 ◽  
Vol 206 (5) ◽  
pp. 329-332 ◽  
Author(s):  
Annette Schäfer ◽  
Wiebke Drewes ◽  
F. Schwägele
Keyword(s):  
Membrane Proteins ◽  
Vitelline Membrane

scholarly journals A novel approach to analyze membrane proteins by laser mass spectrometry: From protein subunits to the integral complex

2007 ◽  
Vol 18 (8) ◽  
pp. 1429-1438 ◽  
Author(s):  
Nina Morgner ◽  
Thomas Kleinschroth ◽  
Hans-Dieter Barth ◽  
Bernd Ludwig ◽  
Bernhard Brutschy
Keyword(s):  
Mass Spectrometry ◽  
Membrane Proteins ◽  
Laser Mass Spectrometry ◽  
Protein Subunits ◽  
Novel Approach
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