scholarly journals Human sperm aneuploidy after exposure to pesticides

2004 ◽  
Vol 67 (3) ◽  
pp. 353-359 ◽  
Author(s):  
Jena L. Smith ◽  
Vincent F. Garry ◽  
Alfred W. Rademaker ◽  
Renée H. Martin
Keyword(s):  
Human Sperm ◽  
Sperm Aneuploidy

Human sperm aneuploidy: FISH analysis in fertile and infertile men

2011 ◽  
Vol 6 (6) ◽  
pp. 609-627 ◽  
Author(s):  
Laura Gambera ◽  
Giuseppe Morgante ◽  
Francesca Serafini ◽  
Anita Stendardi ◽  
Raoul Orvieto ◽  
...  
Keyword(s):  
Human Sperm ◽  
Fish Analysis ◽  
Infertile Men ◽  
Sperm Aneuploidy

Three-probe fluorescence in situ hybridization to assess chromosome X, Y, and 8 aneuploidy in sperm of 14 men from two healthy groups: evidence for a paternal age effect on sperm aneuploidy

1995 ◽  
Vol 7 (4) ◽  
pp. 799 ◽  
Author(s):  
WA Robbins ◽  
JE Baulch ◽  
D Moore ◽  
HU Weier ◽  
D Blakey ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Preliminary Finding ◽  
Human Sperm ◽  
Age Effect ◽  
Chromosome 8 ◽  
Paternal Age ◽  
Sperm Aneuploidy ◽  
Paternal Age Effect

The method of simultaneous three-chromosome fluorescence in situ hybridization (FISH) was developed using repetitive DNA sequence probes for chromosomes 8, X and Y and applied to semen of 14 men from two healthy groups who differed in their average ages (46.8 +/- 3.1 years, n = 4 v. 28.9 +/- 5.0 years, n = 10). The frequencies of disomic sperm determined by FISH compared well with frequencies obtained using the hamster-egg technique for human-sperm cytogenetics and with the frequencies of disomic and diploid sperm reported in previous FISH studies in this laboratory. The two groups of men did not differ in their baseline frequencies of sperm disomic for chromosome 8 (approximately 6.5 per 10(4) sperm), sperm with XY8 aneuploidy (approximately 9.5 per 10(4) sperm), or sperm with autodiploidy XX88 or YY88 (approximately 2 per 10(4) sperm). However, the older group had statistically higher frequencies of sperm carrying sex chromosomal disomy than the younger group (5.1 v. 2.2 per 10(4) sperm for XX8; 5.9 v. 2.0 per 10(4) sperm for YY8; P < 0.005). A recent report from this laboratory of sex-chromosomal aneuploidy in sperm of aged mice provides inter-species corroborating evidence for this preliminary finding of a paternal age effect on sperm aneuploidy in human males.

scholarly journals Exposure to Perfluorinated Compounds and Human Sperm Aneuploidy

2020 ◽  
Vol 2020 (1) ◽  
Author(s):  
H.A. Young ◽  
M.J. Perry ◽  
P. Grandjean ◽  
J. Halling ◽  
N. Jorgensen ◽  
...  
Keyword(s):  
Human Sperm ◽  
Perfluorinated Compounds ◽  
Sperm Aneuploidy

Human sperm aneuploidy after exposure to polycyclic aromatic hydrocarbons

2016 ◽  
Vol 28 (9) ◽  
pp. 1376 ◽  
Author(s):  
Michał Radwan ◽  
Joanna Jurewicz ◽  
Wojciech Sobala ◽  
Sławomir Brzeźnicki ◽  
Paweł Radwan ◽  
...  
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Environmental Exposure ◽  
Aromatic Hydrocarbons ◽  
High Performance ◽  
Sex Chromosome ◽  
In Situ Hybridisation ◽  
Human Sperm ◽  
Cross Sectional ◽  
Sperm Aneuploidy ◽  
Polycyclic Aromatic

The purpose of this cross-sectional study was to investigate whether environmental exposure to polycyclic aromatic hydrocarbons (PAHs) was associated with sperm aneuploidy. A sample of 181 men who attended an infertility clinic for diagnostic purposes and who had a normal semen concentration of 20–300 × 106 spermatozoa mL–1 or slight oligozoospermia (semen concentration of 15–20 × 106 spermatozoa mL–1; WHO 1999) provided urine and semen samples. Analysis of the level of PAH biomarker 1-hydroxypyrene (1-OHP) in urine was performed using high-performance liquid chromatography. Sperm aneuploidy was assessed using multicolour florescence in situ hybridisation (FISH) using DNA probes specific for chromosomes X, Y, 18, 13 and 21. Positive associations were observed between the level of 1-OHP in urine and total sex-chromosome disomy (P = 0.03) and chromosome-18 disomy (P = 0.03). These results suggest that environmental exposure to PAHs may be associated with sperm aneuploidy. This is the first epidemiological study to investigate the relationship between environmental exposure to PAHs and sperm aneuploidy. Therefore, these findings require further replication in other populations using different biomarkers of PAH exposure.

A High Resolution Cytochemical Study of Nuclear ATPase in Human Spermatozoa

1975 ◽  
Vol 33 ◽  
pp. 594-595
Author(s):  
A. Sosa ◽  
L. Calzada
Keyword(s):  
High Resolution ◽  
Atpase Activity ◽  
Nuclear Membrane ◽  
Human Sperm ◽  
Sperm Nucleus ◽  
Human Spermatozoa ◽  
Cytochemical Study ◽  
Membrane Bound ◽  
Sperm Nuclei ◽  
Interchromatin Space

The dependence of nuclear metabolism on the function of the nuclear membrane is not well understood. Whether or not the function of the nuclear membrane is partial or totally responsible of the repressed template activity of human sperm nucleus has not at present been elucidated. One of the membrane-bound enzymatic activities which is concerned with the mechanisms whereby substances are thought to cross cell membranes is adenosintriphosphatase (ATPase). This prompted its characterization and distribution by high resolution photogrammetry on isolated human sperm nuclei. Isolated human spermatozoa nuclei were obtained as previously described. ATPase activity was demonstrated by the method of Wachstein and Meisel modified by Marchesi and Palade. ATPase activity was identified as dense and irregularly distributed granules confined to the internal leaflet of the nuclear membrane. Within the nucleus the appearance of the reaction product occurs as homogenous and dense precipitates in the interchromatin space.

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