Primordial germ cell-somatic cell partnership: A balancing cell signaling act

2001 ◽  
Vol 60 (3) ◽  
pp. 277-280 ◽  
Author(s):  
Abraham L. Kierszenbaum ◽  
Laura L. Tres
Keyword(s):  
Cell Signaling ◽  
Germ Cell ◽  
Somatic Cell ◽  
Primordial Germ Cell

Sall4 Is Essential for Mouse Primordial Germ Cell Specification by Suppressing Somatic Cell Program Genes

Stem Cells ◽  
2014 ◽  
Vol 33 (1) ◽  
pp. 289-300 ◽  
Author(s):  
Yasuka L. Yamaguchi ◽  
Satomi S. Tanaka ◽  
Maho Kumagai ◽  
Yuka Fujimoto ◽  
Takeshi Terabayashi ◽  
...  
Keyword(s):  
Germ Cell ◽  
Somatic Cell ◽  
Primordial Germ Cell ◽  
Cell Specification ◽  
Germ Cell Specification ◽  
Mouse Primordial Germ Cell

scholarly journals Induction of meiosis by embryonic gonadal somatic cells differentiated from pluripotent stem cells

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Haiying Wang ◽  
Linlin Liu ◽  
Chang Liu ◽  
Lingling Wang ◽  
Jiyu Chen ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Germ Cell ◽  
Small Molecules ◽  
Somatic Cell ◽  
Pluripotent Stem Cells ◽  
Primordial Germ Cell ◽  
Somatic Cells ◽  
Embryonic Stem

Abstract Background Depletion of oocytes leads to ovarian aging-associated infertility, endocrine disruption and related diseases. Excitingly, unlimited oocytes can be generated by differentiation of primordial germ cell like cells (PGCLCs) from pluripotent stem cells. Nevertheless, development of oocytes and follicles from PGCLCs relies on developmentally matched gonadal somatic cells, only available from E12.5 embryos in mice. It is therefore imperative to achieve an in vitro source of E12.5 gonadal somatic cells. Methods We explored to identify small molecules, which can induce female embryonic stem cells (ESCs) into gonadal somatic cell like cells. Results Using RNA-sequencing, we identified signaling pathways highly upregulated in E12.5_gonadal somatic cells (E12.5_GSCs). Through searching for the activators of these pathways, we identified small-molecule compounds Vitamin C (Vc) and AM580 in combination (V580) for inducing differentiation of female embryonic stem cells (ESCs) into E12.5_GSC-like cells (E12.5_GSCLCs). After V580 treatment for 6 days and sorted by a surface marker CD63, the cell population yielded a transcriptome profile similar to that of E12.5_GSCs, which promoted meiosis progression and folliculogenesis of primordial germ cells. This approach will contribute to the study of germ cell and follicle development and oocyte production and have implications in potentially treating female infertility. Conclusion ESCs can be induced into embryonic gonadal somatic cell like cells by small molecules.

216. c-kit Expression study: timing of onset in rodent testis and irradiated rat testis model

2005 ◽  
Vol 17 (9) ◽  
pp. 83
Author(s):  
S. Mithra Prabhu ◽  
M. L. Meistrich ◽  
S. Mendis ◽  
E. A. McLaughlin ◽  
K. L. Loveland
Keyword(s):  
Protein Expression ◽  
Germ Cell ◽  
Somatic Cell ◽  
Messenger Rna ◽  
Cell Function ◽  
Primordial Germ Cell ◽  
In Situ Hybridisation ◽  
Hormone Treatment ◽  
Normal Male ◽  
Rat Testis

Primordial germ cell and spermatogonial cell function is essential for normal male fertility. These cells require Sertoli–germ cell interactions, specifically somatic cell-derived stem cell factor (SCF) that acts through the c-kit receptor to govern primordial germ cell migration in the foetus, spermatogonial differentiation during puberty and adulthood, and Leydig cell steroidogenesis. We performed a comprehensive study of the c-kit mRNA expression profile in the pre- and post-pubertal mouse and rat testes by in situ hybridisation. Expression of c-kit mRNA was first visualised in germ cells after birth, with the levels concordant with the number and appearance of the differentiated spermatogonial subtypes in both the rat and the mouse. We also studied c-kit expression in the irradiated adult rat testis, in which only undifferentiated spermatogonia are present. After treatment with Cetrorelix, GnRH antagonist (3 days, 1, 2 and 4 weeks) germ cell maturation is re-initiated. Expression of c-kit messenger RNA was observed in the undifferentiated spermatogonia in both untreated and treated testes sections. In contrast, c-kit protein expression was undetectable until 4 weeks of hormone treatment. This suggests that c-kit mRNA and protein expression are differentially regulated and that protein expression relates to somatic cell function.

scholarly journals AYAM LEHER GUNDUL: SEJARAH, KARAKTERISTIK, DAN KONSERVASI

2020 ◽  
Vol 23 (1) ◽  
pp. 22
Author(s):  
Kostaman T.
Keyword(s):  
Germ Cell ◽  
Primordial Germ Cell

Ayam leher gundul adalah ayam asli/lokal Indonesia yang belum banyak diketahui informasinya dalam halsejarah, potensi, dan karakteristik; sehingga belum dimanfaatkan secara optimal. Ciri yang paling menonjoldari ayam leher gundul adalah tidak mempunyai bulu dibagian lehernya. Untuk mempertahankannya, metode konservasi yang telah dilakukan oleh Balai Penelitian Ternak (Balitnak) adalah dengan memelihara ternak hidup, akan tetapi dengan keterbatasan sarana dan prasarana tidak dapat dilanjutkan. Data performan ayam leher gundul yang dipelihara di Balitnak sudah terkumpul dan ditabulasi. Alternatif konservasi yang dilakukan oleh Balitnak untuk mempertahankan plasma nutfah ayam leher gundul adalah berupa primordial germ cell (PGC) yang dibekukan pada suhu -196 oC yang sewaktu-waktu dibutuhkan dapat ditransfer ke embrio resipien. Sumber PGC yang di koleksi adalah dari darah dan gonad embrio. Berdasarkan potensi yang dimilikinya, ayam leher gundul memiliki performan yang baik, sehingga dapat dimanfaatkan sebagai ayam lokal penghasil daging dan telur. Tulisan ini diharapkan dapat bermanfaat sebagai data pendukung bagi upaya pelestarian dan pengembangan ayam-ayam lokal yang ada di Indonesia, khususnya ayam leher gundul dan umumnya ayam-ayam lokal Indonesia lainnya.

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