Okadaic acid induces premature chromosome condensation reflecting the cell cycle progression in one-cell stage mouse embryos

1993 ◽  
Vol 34 (4) ◽  
pp. 402-415 ◽  
Author(s):  
A. P. Dyban ◽  
P. de Sutter ◽  
Y. Verlinsky
Keyword(s):  
Cell Cycle ◽  
Okadaic Acid ◽  
Cell Cycle Progression ◽  
Chromosome Condensation ◽  
Mouse Embryos ◽  
Premature Chromosome Condensation ◽  
Cell Stage ◽  
Cycle Progression

scholarly journals Aneuploidy during the onset of mouse embryo development

Reproduction ◽  
2020 ◽  
Vol 160 (5) ◽  
pp. 773-782
Author(s):  
Tereza Pauerova ◽  
Lenka Radonova ◽  
Kristina Kovacovicova ◽  
Lucia Novakova ◽  
Michal Skultety ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Early Development ◽  
Cell Cycle Progression ◽  
Developmental Stages ◽  
Model Organism ◽  
Cell Stage ◽  
Cycle Progression ◽  
Animal Reproduction ◽  
Cycle Arrest ◽  
Mouse Embryo Development

Aneuploidy is the most frequent single cause leading into the termination of early development in human and animal reproduction. Although the mouse is frequently used as a model organism for studying the aneuploidy, we have only incomplete information about the frequency of numerical chromosomal aberrations throughout development, usually limited to a particular stage or assumed from the occurrence of micronuclei. In our study, we systematically scored aneuploidy in in vivo mouse embryos, from zygotes up to 16-cell stage, using kinetochore counting assay. We show here that the frequency of aneuploidy per blastomere remains relatively similar from zygotes until 8-cell embryos and then increases in 16-cell embryos. Due to the accumulation of blastomeres, aneuploidy per embryo increases gradually during this developmental period. Our data also revealed that the aneuploidy from zygotes and 2-cell embryos does not propagate further into later developmental stages, suggesting that embryos suffering from aneuploidy are eliminated at this stage. Experiments with reconstituted live embryos revealed, that hyperploid blastomeres survive early development, although they exhibit slower cell cycle progression and suffer frequently from DNA fragmentation and cell cycle arrest.

scholarly journals miR-92a-3p controls cell cycle progression in zebrafish

2019 ◽  
Author(s):  
Christopher E. Presslauer ◽  
Teshome T. Bizuayehu ◽  
Jorge M.O. Fernandes ◽  
Igor S. Babiak
Keyword(s):  
Cell Cycle ◽  
Cell Cycle Progression ◽  
Protein Tyrosine Kinase ◽  
Gonadal Development ◽  
Early Embryo ◽  
Cyclin Dependent Kinase ◽  
Cell Stage ◽  
Cycle Progression ◽  
Early Stages ◽  
Micro Rnas

AbstractBiological functions of micro RNAs (miRNAs) in the early stages of vertebrate development remain largely unknown. In zebrafish, miRNA miR-92a-3p is abundant in the germ cells throughout gonadal development, as well as in ovulated oocytes. Previously, we demonstrated that inhibition of miR-92a-3p in mature ovaries resulted in developmental arrest at the 1-cell stage upon fertilization of the affected oocytes. This suggested functions of miR-92a-3p in early development. In the present study, we identified wee2, an oocyte-specific protein tyrosine kinase, as a target of maternal miR-92a-3p during the early stages of zebrafish embryogenesis. Spatiotemporal co-presence of both miR-92a-3p and wee2 during early embryo development was confirmed by absolute quantification and in situ hybridization. Targeted knockdown of miR-92a-3p in embryos resulted in retarded embryonic development over the first 24 hours. Target validation assays demonstrated that miR-92a-3p interacted with the predicted wee2 3’UTR binding site, which was strongly suppressed by endogenous miR-92a-3p. Our results suggest that miR-92a-3p regulates the abundance of wee2, a cyclin-dependent kinase 1 inhibitor, thus having important role in regulation of the cell cycle during cleavage stages in zebrafish.Summary statementIn zebrafish, maternal miR-92a-3p was demonstrated to suppress translation of wee2, a cyclin-dependent kinase 1 inhibitor which regulates cell cycle progression during the early stages of embryogenesis.

scholarly journals The use of premature chromosome condensation to study in interphase cells the influence of environmental factors on human genetic material

2006 ◽  
Vol 6 ◽  
pp. 1174-1190 ◽  
Author(s):  
Vasiliki I. Hatzi ◽  
Georgia I. Terzoudi ◽  
Christina Paraskevopoulou ◽  
Vasilios Makropoulos ◽  
Demetrios P. Matthopoulos ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Environmental Factors ◽  
Cell Cycle Progression ◽  
Genetic Material ◽  
Chromosome Condensation ◽  
Premature Chromosome Condensation ◽  
Interphase Chromosome ◽  
Diagnosis And Prognosis ◽  
Anthropogenic Environment ◽  
Genotoxic Factors

Nowadays, there is a constantly increasing concern regarding the mutagenic and carcinogenic potential of a variety of harmful environmental factors to which humans are exposed in their natural and anthropogenic environment. These factors exert their hazardous potential in humans' personal (diet, smoking, pharmaceuticals, cosmetics) and occupational environment that constitute part of the anthropogenic environment. It is well known that genetic damage due to these factors has dramatic implications for human health. Since most of the environmental genotoxic factors induce arrest or delay in cell cycle progression, the conventional analysis of chromosomes at metaphase may underestimate their genotoxic potential. Premature Chromosome Condensation (PCC) induced either by means of cell fusion or specific chemicals, enables the microscopic visualization of interphase chromosomes whose morphology depends on the cell cycle stage, as well as the analysis of structural and numerical aberrations at the G1 and G2 phases of the cell cycle. The PCC has been successfully used in problems involving cell cycle analysis, diagnosis and prognosis of human leukaemia, assessment of interphase chromosome malformations resulting from exposure to radiation or chemicals, as well as elucidation of the mechanisms underlying the conversion of DNA damage into chromosomal damage. In this report, particular emphasis is given to the advantages of the PCC methodology used as an alternative to conventional metaphase analysis in answering questions in the fields of radiobiology, biological dosimetry, toxicogenetics, clinical cytogenetics and experimental therapeutics.

scholarly journals Deacetylation and methylation at histone H3 lysine 9 (H3K9) coordinate chromosome condensation during cell cycle progression

2011 ◽  
Vol 31 (4) ◽  
pp. 343-349 ◽  
Author(s):  
Jin-Ah Park ◽  
Ae-Jin Kim ◽  
Yoonsung Kang ◽  
Yu-Jin Jung ◽  
Hyong Kyu Kim ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Cycle Progression ◽  
Histone H3 ◽  
Chromosome Condensation ◽  
Cycle Progression
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