Efficacy of antibodies againstEscherichia coli expressed chimeric recombinant protein encompassing multiple epitopes of zona pellucida glycoproteins to inhibit in vitro human sperm-egg binding

2003 ◽  
Vol 65 (3) ◽  
pp. 309-317 ◽  
Author(s):  
Neela Sivapurapu ◽  
Abhishek Upadhyay ◽  
Akiko Hasegawa ◽  
Koji Koyama ◽  
Satish K. Gupta
Keyword(s):  
Recombinant Protein ◽  
Zona Pellucida ◽  
Human Sperm

scholarly journals A Monoclonal Antibody to Human SP-10 Inhibits In Vitro the Binding of Human Sperm to Hamster Oolemma but Not to Human Zona Pellucida

2000 ◽  
Vol 62 (5) ◽  
pp. 1201-1208 ◽  
Author(s):  
Toshio Hamatani ◽  
Kiyoo Tanabe ◽  
Kiyoshi Kamei ◽  
Nozomi Sakai ◽  
Yurie Yamamoto ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Zona Pellucida ◽  
Human Sperm

Native zona pellucida reactivity and in-vitro effect on human sperm–egg binding with antisera against bonnet monkey ZP1 and ZP3 synthetic peptides

2002 ◽  
Vol 56 (1-2) ◽  
pp. 77-91 ◽  
Author(s):  
Neela Sivapurapu ◽  
Abhishek Upadhyay ◽  
Akiko Hasegawa ◽  
Koji Koyama ◽  
Satish K Gupta
Keyword(s):  
Zona Pellucida ◽  
Synthetic Peptides ◽  
Human Sperm ◽  
Bonnet Monkey ◽  
Vitro Effect

Antibodies generated in response to plasmid DNA encoding zona pellucida glycoprotein-B inhibit in vitro human sperm-egg binding

2002 ◽  
Vol 62 (4) ◽  
pp. 525-533 ◽  
Author(s):  
Archana Rath ◽  
Sangeeta Choudhury ◽  
Akiko Hasegawa ◽  
Koji Koyama ◽  
Satish K. Gupta
Keyword(s):  
Zona Pellucida ◽  
Plasmid Dna ◽  
Human Sperm ◽  
Glycoprotein B ◽  
Dna Encoding

Human ZP3 restores fertility in Zp3 null mice without affecting order-specific sperm binding

Development ◽  
1998 ◽  
Vol 125 (13) ◽  
pp. 2415-2424 ◽  
Author(s):  
T.L. Rankin ◽  
Z.B. Tong ◽  
P.E. Castle ◽  
E. Lee ◽  
R. Gore-Langton ◽  
...  
Keyword(s):  
Zona Pellucida ◽  
Molecular Basis ◽  
Human Sperm ◽  
Apparent Mass ◽  
Sperm Binding ◽  
Mammalian Fertilization ◽  
Vitro Data ◽  
In Vitro Data

The mammalian zona pellucida surrounding ovulated eggs mediates sperm binding at fertilization, provides a postfertilization block to polyspermy, and facilitates passage of pre-implantation embryos down the oviduct. Although the three zona proteins (ZP1, ZP2, ZP3) are well conserved, mammalian fertilization is relatively specific and human sperm do not bind to the mouse zona pellucida. There are considerable in vitro data that ZP3 acts as a primary sperm adhesion molecule in mice and, by analogy, a similar role has been postulated for human ZP3. Genetically altered mice lacking ZP3 (Zp3(tm/tm)) do not form a zona pellucida and are infertile. To rescue this phenotype, transgenic mice expressing human ZP3 (67% identical to mouse ZP3) were produced and bred with Zp3(tm/tm) null mice. The resultant human ZP3 rescue females had chimeric zonae pellucidae composed of mouse ZP1, mouse ZP2 and human ZP3. Human ZP3 expressed in mouse oocytes had an apparent mass (64 kDa) indistinguishable from native human ZP3 and distinct from mouse ZP3 (83 kDa). Despite the presence of human ZP3, human sperm did not bind to the chimeric zona pellucida, and notwithstanding the absence of mouse ZP3, mouse sperm bound to ovulated eggs in vitro and fertility was restored in vivo. These data have implications regarding the molecular basis of mouse and human sperm binding to their respective zonae pellucidae.

A Human Sperm-Zona Pellucida Binding Test Using Oocytes That Failed to Fertilize In Vitro

1989 ◽  
Vol 141 (5) ◽  
pp. 1266-1266
Author(s):  
D.Y. Liu ◽  
A. Lopata ◽  
W.I.H. Johnstone ◽  
H.W.G. Baker
Keyword(s):  
Zona Pellucida ◽  
Human Sperm

A human sperm-zona pellucida binding test using oocytes that failed to fertilize in vitro

1988 ◽  
Vol 50 (5) ◽  
pp. 782-788 ◽  
Author(s):  
De Yi Liu ◽  
Alexander Lopata ◽  
W. Ian H. Johnston ◽  
H.W. Gordon Baker
Keyword(s):  
Zona Pellucida ◽  
Human Sperm

scholarly journals Immunoreactivity and in-vitro effect on human sperm-egg binding of antibodies against peptides corresponding to bonnet monkey zona pellucida-3 glycoprotein

1997 ◽  
Vol 12 (12) ◽  
pp. 2664-2670 ◽  
Author(s):  
A. Afzalpurkar ◽  
H. Shibahara ◽  
A. Hasegawa ◽  
K. Koyama ◽  
S. K. Gupta
Keyword(s):  
Zona Pellucida ◽  
Human Sperm ◽  
Bonnet Monkey ◽  
Vitro Effect
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