Antibodies generated in response to plasmid DNA encoding zona pellucida glycoprotein-B inhibit in vitro human sperm-egg binding

2002 ◽  
Vol 62 (4) ◽  
pp. 525-533 ◽  
Author(s):  
Archana Rath ◽  
Sangeeta Choudhury ◽  
Akiko Hasegawa ◽  
Koji Koyama ◽  
Satish K. Gupta
Keyword(s):  
Zona Pellucida ◽  
Plasmid Dna ◽  
Human Sperm ◽  
Glycoprotein B ◽  
Dna Encoding

scholarly journals A Monoclonal Antibody to Human SP-10 Inhibits In Vitro the Binding of Human Sperm to Hamster Oolemma but Not to Human Zona Pellucida

2000 ◽  
Vol 62 (5) ◽  
pp. 1201-1208 ◽  
Author(s):  
Toshio Hamatani ◽  
Kiyoo Tanabe ◽  
Kiyoshi Kamei ◽  
Nozomi Sakai ◽  
Yurie Yamamoto ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Zona Pellucida ◽  
Human Sperm

Native zona pellucida reactivity and in-vitro effect on human sperm–egg binding with antisera against bonnet monkey ZP1 and ZP3 synthetic peptides

2002 ◽  
Vol 56 (1-2) ◽  
pp. 77-91 ◽  
Author(s):  
Neela Sivapurapu ◽  
Abhishek Upadhyay ◽  
Akiko Hasegawa ◽  
Koji Koyama ◽  
Satish K Gupta
Keyword(s):  
Zona Pellucida ◽  
Synthetic Peptides ◽  
Human Sperm ◽  
Bonnet Monkey ◽  
Vitro Effect

Characterization of immune response in mice to plasmid DNA encoding human sperm associated antigen 9 (SPAG9)

Vaccine ◽  
2006 ◽  
Vol 24 (17) ◽  
pp. 3695-3703 ◽  
Author(s):  
N JAGADISH ◽  
R RANA ◽  
D MISHRA ◽  
M GARG ◽  
R SELVI ◽  
...  
Keyword(s):  
Immune Response ◽  
Plasmid Dna ◽  
Human Sperm ◽  
Dna Encoding

Ultrasound-Enhanced Chemotherapy and Gene Delivery for Glioma Cells

2007 ◽  
Vol 6 (5) ◽  
pp. 433-442 ◽  
Author(s):  
Vladimir G. Zarnitsyn ◽  
Pavel P. Kamaev ◽  
Mark R. Prausnitz
Keyword(s):  
Gene Therapy ◽  
Brain Cancer ◽  
Plasmid Dna ◽  
Glioma Cells ◽  
Intracellular Delivery ◽  
Intracellular Uptake ◽  
Dna Encoding ◽  
Ultrasound Exposure ◽  
Gfp Reporter

Treatment of brain cancer is limited in part by inefficient intracellular delivery of drugs and DNA for chemotherapy and gene therapy, respectively. This study tested the hypothesis that ultrasound may be used to enhance intracellular delivery and efficacy of chemotherapeutics and genes in glioma cells in vitro. First, suitable ultrasound conditions were identified by measuring intracellular uptake of calcein and viability of GS 9L rat gliosarcoma cells after a range of different ultrasound exposures. We selected sonication at 10 J/cm2, which achieved intracellular delivery of ν106 molecules/cell. Next, glial cells were sonicated with varying concentrations of model chemotherapeutics: BCNU and bleomycin. For both drugs, cytotoxicity was increased in a synergistic manner when accompanied by ultrasound exposure. Finally, expression of a plasmid DNA encoding a GFP reporter was increased up to 30-fold when exposed to ultrasound. Altogether, these findings suggest that ultrasound may be useful to increase the efficacy of chemotherapy and gene therapy of glioma cells.

Human ZP3 restores fertility in Zp3 null mice without affecting order-specific sperm binding

Development ◽  
1998 ◽  
Vol 125 (13) ◽  
pp. 2415-2424 ◽  
Author(s):  
T.L. Rankin ◽  
Z.B. Tong ◽  
P.E. Castle ◽  
E. Lee ◽  
R. Gore-Langton ◽  
...  
Keyword(s):  
Zona Pellucida ◽  
Molecular Basis ◽  
Human Sperm ◽  
Apparent Mass ◽  
Sperm Binding ◽  
Mammalian Fertilization ◽  
Vitro Data ◽  
In Vitro Data

The mammalian zona pellucida surrounding ovulated eggs mediates sperm binding at fertilization, provides a postfertilization block to polyspermy, and facilitates passage of pre-implantation embryos down the oviduct. Although the three zona proteins (ZP1, ZP2, ZP3) are well conserved, mammalian fertilization is relatively specific and human sperm do not bind to the mouse zona pellucida. There are considerable in vitro data that ZP3 acts as a primary sperm adhesion molecule in mice and, by analogy, a similar role has been postulated for human ZP3. Genetically altered mice lacking ZP3 (Zp3(tm/tm)) do not form a zona pellucida and are infertile. To rescue this phenotype, transgenic mice expressing human ZP3 (67% identical to mouse ZP3) were produced and bred with Zp3(tm/tm) null mice. The resultant human ZP3 rescue females had chimeric zonae pellucidae composed of mouse ZP1, mouse ZP2 and human ZP3. Human ZP3 expressed in mouse oocytes had an apparent mass (64 kDa) indistinguishable from native human ZP3 and distinct from mouse ZP3 (83 kDa). Despite the presence of human ZP3, human sperm did not bind to the chimeric zona pellucida, and notwithstanding the absence of mouse ZP3, mouse sperm bound to ovulated eggs in vitro and fertility was restored in vivo. These data have implications regarding the molecular basis of mouse and human sperm binding to their respective zonae pellucidae.

A Human Sperm-Zona Pellucida Binding Test Using Oocytes That Failed to Fertilize In Vitro

1989 ◽  
Vol 141 (5) ◽  
pp. 1266-1266
Author(s):  
D.Y. Liu ◽  
A. Lopata ◽  
W.I.H. Johnstone ◽  
H.W.G. Baker
Keyword(s):  
Zona Pellucida ◽  
Human Sperm

scholarly journals Gemini Cationic Lipid-Type Nanovectors Suitable for the Transfection of Therapeutic Plasmid DNA Encoding for Pro-Inflammatory Cytokine Interleukin-12

Pharmaceutics ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 729
Author(s):  
Natalia Sánchez-Arribas ◽  
María Martínez-Negro ◽  
Clara Aicart-Ramos ◽  
Conchita Tros de Ilarduya ◽  
Emilio Aicart ◽  
...  
Keyword(s):  
Plasmid Dna ◽  
Physicochemical Characterization ◽  
Cationic Lipid ◽  
Cationic Lipids ◽  
Interleukin 12 ◽  
Dna Encoding ◽  
Lipid Mixture ◽  
Ample Evidence

Ample evidence exists on the role of interleukin-12 (IL-12) in the response against many pathogens, as well as on its remarkable antitumor properties. However, the unexpected toxicity and disappointing results in some clinical trials are prompting the design of new strategies and/or vectors for IL-12 delivery. This study was conceived to further endorse the use of gemini cationic lipids (GCLs) in combination with zwitterionic helper lipid DOPE (1,2-dioleoyl-sn-glycero-3-phosphatidyl ethanol amine) as nanovectors for the insertion of plasmid DNA encoding for IL-12 (pCMV-IL12) into cells. Optimal GCL formulations previously reported by us were selected for IL-12-based biophysical experiments. In vitro studies demonstrated efficient pCMV-IL12 transfection by GCLs with comparable or superior cytokine levels than those obtained with commercial control Lipofectamine2000*. Furthermore, the nanovectors did not present significant toxicity, showing high cell viability values. The proteins adsorbed on the nanovector surface were found to be mostly lipoproteins and serum albumin, which are both beneficial to increase the blood circulation time. These outstanding results are accompanied by an initial physicochemical characterization to confirm DNA compaction and protection by the lipid mixture. Although further studies would be necessary, the present GCLs exhibit promising characteristics as candidates for pCMV-IL12 transfection in future in vivo applications.

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