Low-methoxyl lemon pectin attenuates inflammatory responses and improves intestinal barrier integrity in caerulein-induced experimental acute pancreatitis

2017 ◽  
Vol 61 (4) ◽  
pp. 1600885 ◽  
Author(s):  
Yajun Sun ◽  
Yue He ◽  
Fei Wang ◽  
Hao Zhang ◽  
Paul de Vos ◽  
...  
Keyword(s):  
Acute Pancreatitis ◽  
Inflammatory Responses ◽  
Intestinal Barrier ◽  
Experimental Acute Pancreatitis ◽  
Barrier Integrity ◽  
Intestinal Barrier Integrity

scholarly journals Chronic opioid use modulates human enteric microbiota and intestinal barrier integrity

Gut Microbes ◽  
2021 ◽  
Vol 13 (1) ◽  
pp. 1946368
Author(s):  
Angélica Cruz-Lebrón ◽  
Ramona Johnson ◽  
Claire Mazahery ◽  
Zach Troyer ◽  
Samira Joussef-Piña ◽  
...  
Keyword(s):  
Intestinal Barrier ◽  
Opioid Use ◽  
Barrier Integrity ◽  
Intestinal Barrier Integrity ◽  
Chronic Opioid Use

scholarly journals Pharmacokinetic Analysis of Carnosic Acid and Carnosol in Standardized Rosemary Extract and the Effect on the Disease Activity Index of DSS-Induced Colitis

Nutrients ◽  
2021 ◽  
Vol 13 (3) ◽  
pp. 773
Author(s):  
Jacob P. Veenstra ◽  
Bhaskar Vemu ◽  
Restituto Tocmo ◽  
Mirielle C. Nauman ◽  
Jeremy J. Johnson
Keyword(s):  
Disease Activity ◽  
Activity Index ◽  
Intestinal Barrier ◽  
Disease Activity Index ◽  
Carnosic Acid ◽  
Rosemary Extract ◽  
Oral Gavage ◽  
Barrier Integrity ◽  
Dietary Phytochemicals ◽  
Intestinal Barrier Integrity

Rosemary extract (RE) is an approved food preservative in the European Union and contains dietary phytochemicals that are beneficial for gastrointestinal health. This study investigated the effects of RE on dextran sodium sulfate (DSS)-induced colitis and also determined the pharmacokinetics of dietary phytochemicals administered to mice via oral gavage. Individual components of rosemary extract were separated and identified by LC–MS/MS. The pharmacokinetics of two major diterpenes from RE, carnosic acid (CA) and carnosol (CL), administered to mice via oral gavage were determined. Then, the effect of RE pre-treatment on the disease activity index (DAI) of DSS-induced colitis in mice was investigated. The study determined that 100 mg/kg RE significantly improved DAI in DSS-induced colitis compared to negative control. Sestrin 2 protein expression, which increased with DSS exposure, was reduced with RE treatment. Intestinal barrier integrity was also shown to improve via fluorescein isothiocyanate (FITC)–dextran administration and Western blot of zonula occludens-1 (ZO-1), a tight junction protein. Rosemary extract was able to improve the DAI of DSS-induced colitis in mice at a daily dose of 100 mg/kg and showed improvement in the intestinal barrier integrity. This study suggests that RE can be an effective preventative agent against IBD.

Intestinal barrier integrity and inflammatory bowel disease: Stem cell‐based approaches to regenerate the barrier

2017 ◽  
Vol 12 (4) ◽  
pp. 923-935 ◽  
Author(s):  
Fredrik E.O. Holmberg ◽  
Jannie Pedersen ◽  
Peter Jørgensen ◽  
Christoffer Soendergaard ◽  
Kim B. Jensen ◽  
...  
Keyword(s):  
Inflammatory Bowel Disease ◽  
Stem Cell ◽  
Bowel Disease ◽  
Intestinal Barrier ◽  
Barrier Integrity ◽  
Inflammatory Bowel ◽  
Intestinal Barrier Integrity

scholarly journals HO-1/CO Maintains Intestinal Barrier Integrity through NF-κB/MLCK Pathway in Intestinal HO-1-/- Mice

2021 ◽  
Vol 2021 ◽  
pp. 1-19
Author(s):  
Zhenling Zhang ◽  
Lijing Zhang ◽  
Qiuping Zhang ◽  
Bojia Liu ◽  
Fang Li ◽  
...  
Keyword(s):  
Molecular Mechanisms ◽  
Intestinal Barrier ◽  
Heme Oxygenase 1 ◽  
Zinc Protoporphyrin ◽  
Barrier Integrity ◽  
Tnf Α ◽  
Intestinal Barrier Integrity ◽  
Deficient Mice

Background. Intestinal barrier injury is an important contributor to many diseases. We previously found that heme oxygenase-1 (HO-1) and carbon monoxide (CO) protect the intestinal barrier. This study is aimed at elucidating the molecular mechanisms of HO-1/CO in barrier loss. Materials and Methods. We induced gut leakiness by injecting carbon tetrachloride (CCl4) to wildtype or intestinal HO-1-deficient mice. In addition, we administrated tumor necrosis factor-α (TNF-α) to cells with gain- or loss-of-HO-1 function. The effects of HO-1/CO maintaining intestinal barrier integrity were investigated in vivo and in vitro. Results. Cobalt protoporphyrin and CO-releasing molecule-2 alleviated colonic mucosal injury and TNF-α levels; upregulated tight junction (TJ) expression; and inhibited epithelial IκB-α degradation and phosphorylation, NF-κB p65 phosphorylation, long MLCK expression, and MLC-2 phosphorylation after administration of CCl4. Zinc protoporphyrin completely reversed these effects. These findings were further confirmed in vitro, using Caco-2 cells with gain- or loss-of-HO-1-function after TNF-α. Pretreated with JSH-23 (NF-κB inhibitor) or ML-7 (long MLCK inhibitor), HO-1 overexpression prevented TNF-α-induced TJ disruption, while HO-1 shRNA promoted TJ damage even in the presence of JSH-23 or ML-7, thus suggesting that HO-1 dependently protected intestinal barrier via the NF-κB p65/MLCK/p-MLC-2 pathway. Intestinal HO-1-deficient mice further demonstrated the effects of HO-1 in maintaining intestinal barrier integrity and its relative mechanisms. Alleviated hepatic fibrogenesis and serum ALT levels finally confirmed the clinical significance of HO-1/CO repairing barrier loss in liver injury. Conclusion. HO-1/CO maintains intestinal barrier integrity through the NF-κB/MLCK pathway. Therefore, the intestinal HO-1/CO-NF-κB/MLCK system is a potential therapeutic target for diseases with a leaky gut.

Role of NHE3 in the Maintenance of Intestinal Barrier Integrity in IL-10-Deficient Mice

2011 ◽  
Vol 140 (5) ◽  
pp. S-634-S-635
Author(s):  
Claire B. Larmonier ◽  
Daniel Laubitz ◽  
Alexis L. Bucknam ◽  
Robert D. Thurston ◽  
Faihza M. Hill ◽  
...  
Keyword(s):  
Intestinal Barrier ◽  
Barrier Integrity ◽  
Intestinal Barrier Integrity ◽  
Deficient Mice

scholarly journals The long-term consequences of antibiotic therapy: Role of colonic short-chain fatty acids (SCFA) system and intestinal barrier integrity

PLoS ONE ◽  
2019 ◽  
Vol 14 (8) ◽  
pp. e0220642 ◽  
Author(s):  
Yuliia Holota ◽  
Taisa Dovbynchuk ◽  
Izumi Kaji ◽  
Igor Vareniuk ◽  
Natalia Dzyubenko ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Antibiotic Therapy ◽  
Intestinal Barrier ◽  
Short Chain Fatty Acids ◽  
Short Chain ◽  
Barrier Integrity ◽  
Intestinal Barrier Integrity ◽  
Long Term Consequences

scholarly journals Yeast fermentate prebiotic improves intestinal barrier integrity during heat stress by modulation of the gut microbiota in rats

2019 ◽  
Vol 127 (4) ◽  
pp. 1192-1206 ◽  
Author(s):  
H.A.G. Ducray ◽  
L. Globa ◽  
O. Pustovyy ◽  
E. Morrison ◽  
V. Vodyanoy ◽  
...  
Keyword(s):  
Heat Stress ◽  
Gut Microbiota ◽  
Intestinal Barrier ◽  
Barrier Integrity ◽  
Intestinal Barrier Integrity

Role of defective methylation reactions in ethanol-induced dysregulation of intestinal barrier integrity

2015 ◽  
Vol 96 (1) ◽  
pp. 30-38 ◽  
Author(s):  
Paul G. Thomes ◽  
Natalia A. Osna ◽  
Sarah M. Bligh ◽  
Dean J. Tuma ◽  
Kusum K. Kharbanda
Keyword(s):  
Intestinal Barrier ◽  
Barrier Integrity ◽  
Intestinal Barrier Integrity
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