Association of non-starch polysaccharides and ferulic acid in grain amaranth (Amaranthus caudatus L.) dietary fiber

2005 ◽  
Vol 49 (6) ◽  
pp. 551-559 ◽  
Author(s):  
Mirko Bunzel ◽  
John Ralph ◽  
Hans Steinhart
2021 ◽  
pp. 107008
Author(s):  
Chong-Chong Wang ◽  
Zhen Yang ◽  
Jun-Jie Xing ◽  
Xiao-Na Guo ◽  
Ke-Xue Zhu

LWT ◽  
2018 ◽  
Vol 91 ◽  
pp. 55-62 ◽  
Author(s):  
Lianyan Huang ◽  
Xiaoshuang Zhang ◽  
Huijuan Zhang ◽  
Jing Wang

2014 ◽  
Vol 60 (1) ◽  
pp. 264-269 ◽  
Author(s):  
Beatriz A. Acosta-Estrada ◽  
Marco A. Lazo-Vélez ◽  
Yazel Nava-Valdez ◽  
Janet A. Gutiérrez-Uribe ◽  
Sergio O. Serna-Saldívar

Genome ◽  
2012 ◽  
Vol 55 (8) ◽  
pp. 623-628 ◽  
Author(s):  
Young-Jun Park ◽  
Tomotaro Nishikawa

The objective of this study was to develop a PCR–RFLP method to identity the cultivated species of grain amaranth based on variations in the sequences of their starch synthase genes. We sequenced the SSSI and GBSSI loci in 126 accessions of cultivated grain amaranth collected from diverse locations around the world. We aligned the gene sequences and searched for restriction enzyme cleavage sites specific to each species for use in the PCR–RFLP analysis. Our analyses indicated that EcoRI would recognize the sequence 5′-GAATT/C-3′ in the SSSI gene from Amaranthus caudatus L., and TaqI would recognize the sequence 5′-T/CGA-3′ in the GBSSI gene from Amaranthus hypochondriacus L. The PCR products obtained using gene-specific primers were 423 bp (SSSI) and 627 or 635 bp (GBSSI) in length. These products were cut with different restriction enzymes resulting in species-specific RFLP patterns that could be used to distinguish among the cultivated grain amaranths. The results clearly showed that A. caudatus and A. hypochondriacus were easily differentiated at the species level using this method. Therefore, the PCR–RFLP method targeting amaranth starch synthase genes is simple and rapid, and it will be a useful tool for the identification of cultivated species of grain amaranth.


2021 ◽  
Vol 5 (Supplement_2) ◽  
pp. 346-346
Author(s):  
Yiwei Ma ◽  
Yuwei Lu ◽  
Keith Petrofsky ◽  
Roger Ruan ◽  
Chi Chen

Abstract Objectives Wheat bran is rich in bioactive components, mainly dietary fiber, micronutrients, and phytochemical contents. However, the health-promoting effects of wheat bran is restricted by the high-degree crosslinking of dietary fiber and low bioavailability of phytochemicals. The present study aims to improve the functions of wheat bran by increasing the levels of soluble fiber and free ferulic acid from optimized processing to examine the metabolic effects of consuming functionalized wheat bran (FWB) in mice. Methods Control wheat bran (CWB) was processed by an optimized combination of milling, high pressure pulverization, and alkali treatment, leading to dramatic increases of soluble fiber and free ferulic acid. Three groups of male mice were fed the control AIN93G diet, and two modified AIN93G diets containing 10% of CWB and 10% FWB, respectively, for 7 days. The effects of CWB and FWB on the mouse metabolome were determined through the LC-MS based metabolomics analysis of feces, liver, serum, and urine samples. Results The processing dramatically improved the function-associated physicochemical properties of wheat bran, including the increases of soluble fiber content and viscosity by milling and high-pressure pulverization and the elevation of free ferulic acid by alkali treatment. FWB feeding elevated microbial SCFAs production, promoted the excretion of bile acids and cholesterol in feces, modified the lipidome in the liver, decreased triacylglycerols and cholesterol in serum, and increased the levels of ferulic acid and microbial metabolites in urine. On the other hand, FWB feeding resulted in the increases of free amino acids in feces and the decreases of essential amino acids, choline and its metabolites in the liver. Conclusions The optimized processing dramatically increased the soluble fiber and free ferulic acid contents of wheat bran, resulting in improved hypocholesterolemic and antioxidant functions of FWB. However, the bioavailability of nutrients, including essential amino acids and choline, and the homeostasis of lipidome could be negatively affected by FWB diet. These double-edged metabolic effects warrant further investigations on how to achieve the balance between the functionalization of bioactive components and the disruption of nutrient bioavailability in wheat bran processing. Funding Sources NIFA project MIN-18–125.


2015 ◽  
Author(s):  
Markus G. Stetter ◽  
Thomas Müller ◽  
Karl J. Schmid

AbstractThe process of domestication leads to major morphological and genetic changes, which in combination are known as domestication syndrome that differentiates crops from their wild ancestors. We characterized the genomic and phenotypic diversity of the South American grain amaranthAmaranthus caudatus, which has been cultivated for thousands of years and is one of the three native grain amaranths of South and Central America. Previously, several models of domestication were proposed including a domestication from the close relatives and putative ancestorsA. hybridusorA. quitensis. To investigate the evolutionary relationship ofA. caudatusand its two close relatives, we genotyped 119 amaranth accessions of the three species from the Andean region using genotyping-by-sequencing (GBS) and compared phenotypic variation in two domestication-related traits, seed size and seed color. The analysis of 9,485 SNPs revealed a strong genetic differentiation of cultivatedA. caudatusfrom the relativesA. hybridusandA. quitensis. The two relatives did not cluster according to the species assigment but formed mixed groups according to their geographic origin in Ecuador and Peru, respectively.A. caudatushad a higher genetic diversity than its close relatives and shared a high proportion of polymorphisms with their wild relatives consistent with the absence of a strong bottleneck or a high level of recent gene flow. Genome sizes and seed sizes were not significantly different betweenA. caudatusand its relatives, although a genetically distinct group ofA. caudatusfrom Bolivia had significantly larger seeds. We conclude that despite a long history of human cultivation and selection for white grain color,A. caudatusshows a weak genomic and phenotypic domestication syndrome and is an incompletely domesticated species because of weak selection or high levels of gene flow from its sympatric close undomesticated relatives that counteracted the fixation of key domestication traits.


2017 ◽  
Vol 26 (3) ◽  
pp. 871-886 ◽  
Author(s):  
Markus G. Stetter ◽  
Thomas Müller ◽  
Karl J. Schmid

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