In vivo measurement of oxygen-derived free radicals during reperfusion injury

Microsurgery ◽  
2002 ◽  
Vol 22 (3) ◽  
pp. 108-113 ◽  
Author(s):  
Rolf Büttemeyer ◽  
Andreas W. Philipp ◽  
Julian W. Mall ◽  
Bixia Ge ◽  
Frieder W. Scheller ◽  
...  
Keyword(s):  
Free Radicals ◽  
Reperfusion Injury ◽  
In Vivo Measurement ◽  
Oxygen Derived Free Radicals

scholarly journals Oxygen-derived free radicals stimulate osteoclastic bone resorption in rodent bone in vitro and in vivo.

1990 ◽  
Vol 85 (3) ◽  
pp. 632-639 ◽  
Author(s):  
I R Garrett ◽  
B F Boyce ◽  
R O Oreffo ◽  
L Bonewald ◽  
J Poser ◽  
...  
Keyword(s):  
Free Radicals ◽  
Bone Resorption ◽  
Osteoclastic Bone Resorption ◽  
Oxygen Derived Free Radicals

scholarly journals Reappraisal of the e.p.r. signals in (post)-ischaemic cardiac tissue

1989 ◽  
Vol 264 (3) ◽  
pp. 687-694 ◽  
Author(s):  
A M van der Kraaij ◽  
J F Koster ◽  
W R Hagen
Keyword(s):  
Electron Transfer ◽  
Free Radicals ◽  
Reperfusion Injury ◽  
Respiratory Chain ◽  
Cardiac Tissue ◽  
Direct Detection ◽  
Mitochondrial Respiratory Chain ◽  
Isolated Rat Heart ◽  
Oxygen Derived Free Radicals ◽  
Rat Hearts

The present study was designed to measure directly, using e.p.r. spectroscopy, oxygen-derived free radicals in (post)-ischaemic or (post)-anoxic rat hearts. Rat hearts were rapidly freeze-clamped at 77 K under normoxic, anoxic, ischaemic or reperfusion conditions. The samples were measured at three different temperatures (13, 77 and 115 K) and at several microwave power levels, and were compared with isolated rat heart mitochondria. Samples were prepared both by grinding and as tissue cuts. The two preparation techniques gave identical e.p.r. results, which excludes the occurrence of grinding artifacts. No free radical signals linked to reperfusion injury were detected. Several electron transfer centres known in the mitochondrial respiratory chain were measured. The signals previously assigned to post-ischaemic reperfusion injury were found to originate from electron transfer centres of the respiratory chain, predominantly the iron-sulphur cluster S-1 in succinate dehydrogenase. The differences in signal intensity between normoxic, ischaemic and reperfused hearts were found to result from the different redox stages of these centres under the various conditions tested. These findings do not necessarily imply that oxygen-derived free radicals are not formed in cardiac tissue during (post)-ischaemic reperfusion. The constitutive background of paramagnetism from the respiratory chain, however, seriously hampers the direct detection of comparatively low concentrations of free radicals in cardiac tissue. It is therefore expedient to focus future experiments in this field on the use of spin-trapping agents.

In vivo measurement of radical scavenger efficacy of vitamins C and E in a pig model of pulmonary reperfusion injury

2001 ◽  
Vol 33 (1-2) ◽  
pp. 926-930 ◽  
Author(s):  
F.M Wagner ◽  
A.T Weber ◽  
K Ploetze ◽  
F Schubert ◽  
S Albrecht ◽  
...  
Keyword(s):  
Reperfusion Injury ◽  
Pig Model ◽  
Radical Scavenger ◽  
In Vivo Measurement
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