Plumbagin induces reactive oxygen species, which mediate apoptosis in human cervical cancer cells

2004 ◽  
Vol 40 (4) ◽  
pp. 201-211 ◽  
Author(s):  
Priya Srinivas ◽  
Gokul Gopinath ◽  
Asoke Banerji ◽  
Abhilash Dinakar ◽  
Gopal Srinivas
Keyword(s):  
Reactive Oxygen Species ◽  
Cervical Cancer ◽  
Cancer Cells ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Cervical Cancer Cells ◽  
Human Cervical Cancer Cells ◽  
Human Cervical Cancer

Curcumin induces ER stress-mediated apoptosis through selective generation of reactive oxygen species in cervical cancer cells

2015 ◽  
Vol 55 (5) ◽  
pp. 918-928 ◽  
Author(s):  
Boyun Kim ◽  
Hee Seung Kim ◽  
Eun-Ji Jung ◽  
Jung Yun Lee ◽  
Benjamin K. Tsang ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Cervical Cancer ◽  
Er Stress ◽  
Cancer Cells ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Cervical Cancer Cells ◽  
Selective Generation

scholarly journals Nisin induces apoptosis in cervical cancer cells via reactive oxygen species generation and mitochondrial membrane potential changes

2022 ◽  
Author(s):  
Houri Sadri ◽  
Mahmoud Aghaei ◽  
Vajihe Akbari
Keyword(s):  
Reactive Oxygen Species ◽  
Cervical Cancer ◽  
Membrane Potential ◽  
Cancer Cells ◽  
Mitochondrial Membrane Potential ◽  
Mtt Assay ◽  
Mitochondrial Membrane ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Cervical Cancer Cells

Nisin, an antimicrobial peptide produced by Lactococcus lactis, is widely used as a safe food preservative and has been recently attracting the attention of many researchers as a potential anticancer agent. The cytotoxicity of nisin against HeLa, OVCAR-3, SK-OV-3, and HUVEC cells was evaluated using MTT assay. The apoptotic effect of nisin was identified by Annexin-V/propidium iodide assay, and then it was further confirmed by western blotting analysis, mitochondrial membrane potential (ΔΨm) analysis, and reactive oxygen species (ROS) assay. The MTT assay showed concentration-dependent cytotoxicity of nisin towards cancer cell lines, with the IC50 values of 11.5-23 µM, but less toxicity against normal endothelial cells. Furthermore, treatment of cervical cancer cells with 12 µM nisin significantly (P<0.05) increased the Bax/Bcl-2 ratio (4.9-fold), reduced ΔΨm (70%), and elevated ROS levels (1.7-fold). These findings indicated that nisin might have anticancer and apoptogenic activities through mitochondrial dysfunction and oxidative stress damage in cervical cancer cells.

Sign in / Sign up

Export Citation Format

Share Document