scholarly journals Exogenous fatty acids alter phospholipid composition, membrane permeability, capacity for biofilm formation, and antimicrobial peptide susceptibility in Klebsiella pneumoniae

2018 ◽  
Vol 8 (2) ◽  
pp. e00635 ◽  
Author(s):  
Chelsea R. Hobby ◽  
Joshua L. Herndon ◽  
Colton A. Morrow ◽  
Rachel E. Peters ◽  
Steven J. K. Symes ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Klebsiella Pneumoniae ◽  
Antimicrobial Peptide ◽  
Membrane Permeability ◽  
Biofilm Formation ◽  
Phospholipid Composition

scholarly journals Exogenous polyunsaturated fatty acids (PUFAs) alter phospholipid composition, membrane permeability, biofilm formation and motility in Acinetobacter baumannii

Microbiology ◽  
2017 ◽  
Vol 163 (11) ◽  
pp. 1626-1636 ◽  
Author(s):  
Adrianna E. Eder ◽  
Saba A. Munir ◽  
Chelsea R. Hobby ◽  
Derek M. Anderson ◽  
Joshua L. Herndon ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Polyunsaturated Fatty Acids ◽  
Acinetobacter Baumannii ◽  
Membrane Permeability ◽  
Biofilm Formation ◽  
Phospholipid Composition

scholarly journals Exogenous Polyunsaturated Fatty Acids Impact Membrane Remodeling and Affect Virulence Phenotypes among Pathogenic Vibrio Species

2017 ◽  
Vol 83 (22) ◽  
Author(s):  
Anna R. Moravec ◽  
Andrew W. Siv ◽  
Chelsea R. Hobby ◽  
Emily N. Lindsay ◽  
Layla V. Norbash ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Polyunsaturated Fatty Acids ◽  
Membrane Permeability ◽  
Stress Responses ◽  
Biofilm Formation ◽  
Membrane Lipids ◽  
Regulation Of Gene Expression ◽  
Exogenous Fatty Acid ◽  
Content Type

ABSTRACT The pathogenic Vibrio species (V. cholerae, V. parahaemolyticus, and V. vulnificus) represent a constant threat to human health, causing foodborne and skin wound infections as a result of ingestion of or exposure to contaminated water and seafood. Recent studies have highlighted Vibrio's ability to acquire fatty acids from environmental sources and assimilate them into cell membranes. The possession and conservation of such machinery provokes consideration of fatty acids as important factors in the pathogenic lifestyle of Vibrio species. The findings here link exogenous fatty acid exposure to changes in bacterial membrane phospholipid structure, permeability, phenotypes associated with virulence, and consequent stress responses that may impact survival and persistence of pathogenic Vibrio species. Polyunsaturated fatty acids (PUFAs) (ranging in carbon length and unsaturation) supplied in growth medium were assimilated into bacterial phospholipids, as determined by thin-layer chromatography and liquid chromatography-mass spectrometry. The incorporation of fatty acids variably affected membrane permeability, as judged by uptake of the hydrophobic compound crystal violet. For each species, certain fatty acids were identified as affecting resistance to antimicrobial peptide treatment. Significant fluctuations were observed with regard to both motility and biofilm formation following growth in the presence of individual PUFAs. Our results illustrate the important and complex roles of exogenous fatty acids in the membrane physiology and virulence of a bacterial genus that inhabits aquatic and host environments containing an abundance of diverse fatty acids. IMPORTANCE Bacterial responses to fatty acids include, but are not limited to, degradation for metabolic gain, modification of membrane lipids, alteration of protein function, and regulation of gene expression. Vibrio species exhibit significant diversity with regard to the machinery known to participate in the uptake and incorporation of fatty acids into their membranes. Both aquatic and host niches occupied by Vibrio are rife with various free fatty acids and fatty acid-containing lipids. The roles of fatty acids in the environmental survival and pathogenesis of bacteria have begun to emerge and are expected to expand significantly. The current study demonstrates the responsiveness of V. cholerae, V. parahaemolyticus, and V. vulnificus to exogenous PUFAs. In addition to phospholipid remodeling, PUFA assimilation impacts membrane permeability, motility, biofilm formation, and resistance to polymyxin B.

scholarly journals Exogenous polyunsaturated fatty acids (PUFAs) promote changes in growth, phospholipid composition, membrane permeability and virulence phenotypes in Escherichia coli

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Joshua L. Herndon ◽  
Rachel E. Peters ◽  
Rachel N. Hofer ◽  
Timothy B. Simmons ◽  
Steven J. Symes ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Fatty Acids ◽  
Fatty Acid ◽  
Membrane Permeability ◽  
Phospholipid Composition ◽  
Acid Oxidation ◽  
Gram Negative Bacteria ◽  
Membrane Phospholipids ◽  
Gram Negative ◽  
E Coli

Abstract Background The utilization of exogenous fatty acids by Gram-negative bacteria has been linked to many cellular processes, including fatty acid oxidation for metabolic gain, assimilation into membrane phospholipids, and control of phenotypes associated with virulence. The expanded fatty acid handling capabilities have been demonstrated in several bacteria of medical importance; however, a survey of the polyunsaturated fatty acid responses in the model organism Escherichia coli has not been performed. The current study examined the impacts of exogenous fatty acids on E. coli. Results All PUFAs elicited higher overall growth, with several fatty acids supporting growth as sole carbon sources. Most PUFAs were incorporated into membrane phospholipids as determined by Ultra performance liquid chromatography-mass spectrometry, whereas membrane permeability was variably affected as measured by two separate dye uptake assays. Biofilm formation, swimming motility and antimicrobial peptide resistance were altered in the presence of PUFAs, with arachidonic and docosahexaenoic acids eliciting strong alteration to these phenotypes. Conclusions The findings herein add E. coli to the growing list of Gram-negative bacteria with broader capabilities for utilizing and responding to exogenous fatty acids. Understanding bacterial responses to PUFAs may lead to microbial behavioral control regimens for disease prevention.

scholarly journals Exogenous polyunsaturated fatty acids (PUFAs) promote changes in growth, phospholipid composition, membrane permeability and virulence phenotypes in Escherichia coli

2020 ◽  
Author(s):  
Joshua Herndon ◽  
Rachel Peters ◽  
Rachel Hofer ◽  
Tim Simmons ◽  
Steven Symes ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Fatty Acids ◽  
Fatty Acid ◽  
Membrane Permeability ◽  
Phospholipid Composition ◽  
Acid Oxidation ◽  
Gram Negative Bacteria ◽  
Membrane Phospholipids ◽  
Gram Negative ◽  
E Coli

Abstract Background: The utilization of exogenous fatty acids by Gram-negative bacteria has been linked to many cellular processes, including fatty acid oxidation for metabolic gain, assimilation into membrane phospholipids, and control of phenotypes associated with virulence. The expanded fatty acid handling capabilities have been demonstrated in several bacteria of medical importance; however, a survey of the polyunsaturated fatty acid responses in the model organism Escherichia coli has not been performed. The current study examined the impacts of exogenous fatty acids on E. coli. Results: All PUFAs elicited higher overall growth, with several fatty acids supporting growth as sole carbon sources. Most PUFAs were incorporated into membrane phospholipids as determined by ultra performance liquid chromatography-mass spectrometry, whereas membrane permeability was variably affected as measured by two separate dye uptake assays. Biofilm formation, swimming motility and antimicrobial peptide resistance were altered in the presence of PUFAs, with arachidonic and docosahexaenoic acids eliciting strong alteration to these phenotypes. Conclusions: The findings herein add E. coli to the growing list of Gram-negative bacteria with broader capabilities for utilizing and responding to exogenous fatty acids. Understanding bacterial responses to PUFAs may lead to microbial behavioral control regimens for disease prevention.

scholarly journals Exogenous Polyunsaturated Fatty Acids (PUFAs) Promote Changes in Growth, Phospholipid Composition, Membrane Permeability and Virulence Phenotypes in Escherichia Coli

2020 ◽  
Author(s):  
Joshua Herndon ◽  
Rachel Peters ◽  
Rachel Hofer ◽  
Steven Symes ◽  
David Giles
Keyword(s):  
Escherichia Coli ◽  
Fatty Acids ◽  
Fatty Acid ◽  
Membrane Permeability ◽  
Phospholipid Composition ◽  
Acid Oxidation ◽  
Gram Negative Bacteria ◽  
Membrane Phospholipids ◽  
Gram Negative ◽  
E Coli

Abstract Background: The utilization of exogenous fatty acids by Gram-negative bacteria has been linked to many cellular processes, including fatty acid oxidation for metabolic gain, assimilation into membrane phospholipids, and control of phenotypes associated with virulence. The expanded fatty acid handling capabilities have been demonstrated in several bacteria of medical importance; however, a survey of the polyunsaturated fatty acid responses in the model organism Escherichia coli has not been performed. The current study examined the impacts of exogenous fatty acids on E. coli.Results: All PUFAs elicited higher overall growth, with several fatty acids supporting growth as sole carbon sources. Most PUFAs were incorporated into membrane phospholipids as determined by thin-layer chromatography and UPLC-mass spectrometry, whereas membrane permeability was variably affected as measured by two separate dye uptake assays. Biofilm formation, swimming motility and antimicrobial peptide resistance were altered in the presence of PUFAs, with arachidonic and docosahexaenoic acids eliciting strong alteration to these phenotypes.Conclusions: The findings herein add E. coli to the growing list of Gram-negative bacteria with broader capabilities for utilizing and responding to exogenous fatty acids. Understanding bacterial responses to PUFAs may lead to microbial behavioral control regimens for disease prevention.

scholarly journals Exogenous polyunsaturated fatty acids (PUFAs) promote changes in growth, phospholipid composition, membrane permeability and virulence phenotypes in Escherichia coli

2020 ◽  
Author(s):  
Joshua Herndon ◽  
Rachel Peters ◽  
Rachel Hofer ◽  
Tim Simmons ◽  
Steven Symes ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Fatty Acids ◽  
Fatty Acid ◽  
Membrane Permeability ◽  
Phospholipid Composition ◽  
Acid Oxidation ◽  
Gram Negative Bacteria ◽  
Membrane Phospholipids ◽  
Gram Negative ◽  
E Coli

Abstract Background: The utilization of exogenous fatty acids by Gram-negative bacteria has been linked to many cellular processes, including fatty acid oxidation for metabolic gain, assimilation into membrane phospholipids, and control of phenotypes associated with virulence. The expanded fatty acid handling capabilities have been demonstrated in several bacteria of medical importance; however, a survey of the polyunsaturated fatty acid responses in the model organism Escherichia coli has not been performed. The current study examined the impacts of exogenous fatty acids on E. coli. Results: All PUFAs elicited higher overall growth, with several fatty acids supporting growth as sole carbon sources. Most PUFAs were incorporated into membrane phospholipids as determined by Ultra performance liquid chromatography-mass spectrometry, whereas membrane permeability was variably affected as measured by two separate dye uptake assays. Biofilm formation, swimming motility and antimicrobial peptide resistance were altered in the presence of PUFAs, with arachidonic and docosahexaenoic acids eliciting strong alteration to these phenotypes. Conclusions: The findings herein add E. coli to the growing list of Gram-negative bacteria with broader capabilities for utilizing and responding to exogenous fatty acids. Understanding bacterial responses to PUFAs may lead to microbial behavioral control regimens for disease prevention.

Cm38: A New Antimicrobial Peptide Active Against Klebsiella pneumoniae is Homologous to Cn11

2015 ◽  
Vol 22 (2) ◽  
pp. 164-172 ◽  
Author(s):  
Duenas-Cuellar, R.A. ◽  
Kushmerick, C. ◽  
Naves, L.A. ◽  
Batista, I.F.C. ◽  
Guerrero-Vargas, J.A. ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Antimicrobial Peptide

Difference in biofilm formation between carbapenem-resistant and carbapenem-sensitive Klebsiella pneumoniae based on analysis of mrkH distribution

2021 ◽  
Vol 152 ◽  
pp. 104743
Author(s):  
Renchi Fang ◽  
Haiyang Liu ◽  
Xiucai Zhang ◽  
Guofeng Dong ◽  
Jiahui Li ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Biofilm Formation ◽  
Carbapenem Resistant
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