scholarly journals Quorum-dependent transfer of the opine-catabolic plasmid pAoF64/95 is regulated by a novel mechanism involving inhibition of the TraR antiactivator TraM

2018 ◽  
Vol 8 (1) ◽  
pp. e00625 ◽  
Author(s):  
Margaret E. Wetzel ◽  
Robert E. Asenstorfer ◽  
Max E. Tate ◽  
Stephen K. Farrand
Keyword(s):  
Catabolic Plasmid

scholarly journals Genomic and Functional Analysis of the IncP-9 Naphthalene-Catabolic Plasmid NAH7 and Its Transposon Tn4655 Suggests Catabolic Gene Spread by a Tyrosine Recombinase

2006 ◽  
Vol 188 (11) ◽  
pp. 4057-4067 ◽  
Author(s):  
Masahiro Sota ◽  
Hirokazu Yano ◽  
Akira Ono ◽  
Ryo Miyazaki ◽  
Hidenori Ishii ◽  
...  
Keyword(s):  
Sequence Data ◽  
Gene Clusters ◽  
Catabolic Gene ◽  
Genetic Determinants ◽  
Tyrosine Recombinase ◽  
Site Specific ◽  
Recombination System ◽  
Catabolic Plasmid ◽  
Transmissible Plasmid ◽  
Catabolic Plasmids

ABSTRACT The naphthalene-catabolic (nah) genes on the incompatibility group P-9 (IncP-9) self-transmissible plasmid NAH7 from Pseudomonas putida G7 are some of the most extensively characterized genetic determinants for bacterial aerobic catabolism of aromatic hydrocarbons. In contrast to the detailed studies of its catabolic cascade and enzymatic functions, the biological characteristics of plasmid NAH7 have remained unclear. Our sequence determination in this study together with the previously deposited sequences revealed the entire structure of NAH7 (82,232 bp). Comparison of NAH7 with two other completely sequenced IncP-9 catabolic plasmids, pDTG1 and pWW0, revealed that the three plasmids share very high nucleotide similarities in a 39-kb region encoding the basic plasmid functions (the IncP-9 backbone). The backbone of NAH7 is phylogenetically more related to that of pDTG1 than that of pWW0. These three plasmids carry their catabolic gene clusters at different positions on the IncP-9 backbone. All of the NAH7-specified nah genes are located on a class II transposon, Tn4655. Our analysis of the Tn4655-encoded site-specific recombination system revealed that (i) a novel tyrosine recombinase, TnpI, catalyzed both the intra- and intermolecular recombination between two copies of the attI site, (ii) the functional attI site was located within a 119-bp segment, and (iii) the site-specific strand exchange occurred within a 30-bp segment in the 41-bp CORE site. Our results and the sequence data of other naphthalene-catabolic plasmids, pDTG1 and pND6-1, suggest a potential role of the TnpI-attI recombination system in the establishment of these catabolic plasmids.

scholarly journals Chromosomal gene capture mediated by the Pseudomonas putida TOL catabolic plasmid.

1994 ◽  
Vol 176 (15) ◽  
pp. 4635-4641 ◽  
Author(s):  
M I Ramos-González ◽  
M A Ramos-Díaz ◽  
J L Ramos
Keyword(s):  
Pseudomonas Putida ◽  
Chromosomal Gene ◽  
Gene Capture ◽  
Catabolic Plasmid

Conjugative transfer of naphthalene-catabolic plasmid NAH7 regulated by nitrogen-related phosphotransferase system of recipient cells

2010 ◽  
Vol 150 ◽  
pp. 269-269
Author(s):  
Kei Inoue ◽  
Ryo Miyazaki ◽  
Masatoshi Miyakoshi ◽  
Yosiyuki Ohtsubo ◽  
Yuji Nagata ◽  
...  
Keyword(s):  
Conjugative Transfer ◽  
Phosphotransferase System ◽  
Catabolic Plasmid

scholarly journals H-NS Family Proteins Drastically Change Their Targets in Response to the Horizontal Transfer of the Catabolic Plasmid pCAR1

2020 ◽  
Vol 11 ◽  
Author(s):  
Taisuke Nakamura ◽  
Chiho Suzuki-Minakuchi ◽  
Hibiki Kawano ◽  
Yu Kanesaki ◽  
Shinji Kawasaki ◽  
...  
Keyword(s):  
Horizontal Transfer ◽  
Catabolic Plasmid

scholarly journals Two Opines Control Conjugal Transfer of an Agrobacterium Plasmid by Regulating Expression of Separate Copies of the Quorum-Sensing Activator Gene traR

2002 ◽  
Vol 184 (4) ◽  
pp. 1121-1131 ◽  
Author(s):  
Philippe Oger ◽  
Stephen K. Farrand
Keyword(s):  
Quorum Sensing ◽  
Regulatory System ◽  
Homoserine Lactone ◽  
Conjugal Transfer ◽  
Environmental Cues ◽  
Upstream Gene ◽  
Catabolic Plasmid ◽  
Ti Plasmids ◽  
Control Transfer ◽  
Crown Gall Tumors

ABSTRACT Conjugal transfer of Ti plasmids from Agrobacterium spp. is controlled by a hierarchical regulatory system designed to sense two environmental cues. One signal, a subset of the opines produced by crown gall tumors initiated on plants by the pathogen, serves to induce production of the second, an acyl-homoserine lactone quorum-sensing signal, the quormone, produced by the bacterium itself. This second signal activates TraR, and this transcriptional activator induces expression of the tra regulon. Opines control transfer because the traR gene is a member of an operon the expression of which is regulated by the conjugal opine. Among the Ti plasmid systems studied to date, only one of the two or more opine families produced by the associated tumor induces transfer. However, two chemically dissimilar opines, nopaline and agrocinopines A and B, induce transfer of the opine catabolic plasmid pAtK84b found in the nonpathogenic Agrobacterium radiobacter isolate K84. In this study we showed that this plasmid contains two copies of traR, and each is associated with a different opine-regulated operon. One copy, traR noc, is the last gene of the nox operon and was induced by nopaline but not by agrocinopines A and B. Mutating traR noc abolished induction of transfer by nopaline but not by the agrocinopines. A mutation in ocd, an upstream gene of the nox operon, abolished utilization of nopaline and also induction of transfer by this opine. The second copy, traR acc, is located in an operon of four genes and was induced by agrocinopines A and B but not by nopaline. Genetic analysis indicated that this gene is required for induction of transfer by agrocinopines A and B but not by nopaline. pAtK84b with mutations in both traR genes was not induced for transfer by either opine. However, expression of a traR gene in trans to this plasmid resulted in opine-independent transfer. The association of traR noc with nox is unique, but the operon containing traR acc is related to the arc operons of pTiC58 and pTiChry5, two Ti plasmids inducible for transfer by agrocinopines A-B and C-D, respectively. We conclude that pAtK84b codes for two independently functioning copies of traR, each regulated by a different opine, thus accounting for the activation of the transfer system of this plasmid by the two opine types.

Nucleotide sequence, organization and characterization of the (halo)aromatic acid catabolic plasmid pA81 from Achromobacter xylosoxidans A8

2008 ◽  
Vol 159 (2) ◽  
pp. 118-127 ◽  
Author(s):  
Vera Jencova ◽  
Hynek Strnad ◽  
Zdenek Chodora ◽  
Pavel Ulbrich ◽  
Cestmir Vlcek ◽  
...  
Keyword(s):  
Nucleotide Sequence ◽  
Aromatic Acid ◽  
Achromobacter Xylosoxidans ◽  
Sequence Organization ◽  
Catabolic Plasmid
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