Three‐dimensional quantitative mass spectrometry imaging in complex system: From subcellular to whole organism

2020 ◽  
Author(s):  
Chao Zhao ◽  
Zongwei Cai
Keyword(s):  
Complex System ◽  
Mass Spectrometry ◽  
Mass Spectrometry Imaging ◽  
Three Dimensional ◽  
Quantitative Mass Spectrometry

Mass spectrometry imaging goes three dimensional

2017 ◽  
Vol 14 (12) ◽  
pp. 1139-1140 ◽  
Author(s):  
Klaus Dreisewerd ◽  
Joanne Y Yew
Keyword(s):  
Mass Spectrometry ◽  
Mass Spectrometry Imaging ◽  
Three Dimensional

scholarly journals Three dimensional secondary ion mass spectrometry imaging (3D-SIMS) of Aedes aegypti ovarian follicles

2019 ◽  
Vol 34 (5) ◽  
pp. 874-883 ◽  
Author(s):  
Anthony Castellanos ◽  
Cesar E. Ramirez ◽  
Veronika Michalkova ◽  
Marcela Nouzova ◽  
Fernando G. Noriega ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Aedes Aegypti ◽  
Mass Spectrometry Imaging ◽  
Secondary Ion Mass Spectrometry ◽  
Three Dimensional ◽  
Vital Role ◽  
Reproductive Maturation ◽  
Sugar Feeding ◽  
Nutrient Reserves ◽  
Secondary Ion

The mobilization of nutrient reserves into the ovaries of Aedes aegypti mosquitoes after sugar-feeding plays a vital role in the female's reproductive maturation.

Abstract A114: Quantitative mass spectrometry imaging: A game changer in the pharmaceutical industry

2019 ◽  
Author(s):  
Rima Ait-Belkacem ◽  
Guillaume Hochart ◽  
Joseph Marini ◽  
Aurore Tomezyk ◽  
p Mantefeul ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Pharmaceutical Industry ◽  
Mass Spectrometry Imaging ◽  
Quantitative Mass Spectrometry ◽  
Game Changer

scholarly journals Quantitative Mass Spectrometry Imaging Reveals Mutation Status-independent Lack of Imatinib Penetration into Liver Metastases of Gastrointestinal Stromal Tumors

2019 ◽  
Author(s):  
Denis Abu Sammour ◽  
Christian Marsching ◽  
Alexander Geisel ◽  
Katrin Erich ◽  
Sandra Schulz ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liver Metastases ◽  
Gastrointestinal Stromal Tumors ◽  
Mass Spectrometry Imaging ◽  
Drug Disposition ◽  
Clinical Specimen ◽  
Calibration Model ◽  
Quantitative Mass Spectrometry ◽  
Mutation Status ◽  
Stromal Tumors

AbstractMass spectrometry imaging (MSI) is an enabling technology for label-free drug disposition studies at high spatial resolution in life science- and pharmaceutical research. We present the first extensive clinical matrix-assisted laser desorption/ionization (MALDI) quantitative mass spectrometry imaging (qMSI) study of drug uptake and distribution in clinical specimen, analyzing 56 specimens of tumor and corresponding non-tumor tissues from 28 imatinib-treated patients with biopsy-proven gastrointestinal stromal tumors (GIST). For validation, we compared MALDI-TOF-qMSI with conventional UPLC-ESI-QTOF-MS-based quantification from tissue extracts and with ultra-high resolution MALDI-FTICR-qMSI. We introduced a novel generalized nonlinear calibration model of drug quantities based on focused computational evaluation of drug-containing areas that enabled better data fitting and assessment of the inherent method nonlinearities. Imatinib tissue spatial maps revealed striking inefficiency in drug penetration into GIST liver metastases even though the corresponding healthy liver tissues in the vicinity showed abundant imatinib levels beyond the limit of quantification (LOQ), thus providing evidence for secondary drug resistance independent of mutation status. Taken together, these findings underline the important application of MALDI-qMSI for studying the spatial distribution of molecularly targeted therapeutics in oncology.

scholarly journals Experimental and Data Analysis Considerations for Three-Dimensional Mass Spectrometry Imaging in Biomedical Research

2020 ◽  
Author(s):  
D. R. N. Vos ◽  
S. R. Ellis ◽  
B. Balluff ◽  
R. M. A. Heeren
Keyword(s):  
Mass Spectrometry ◽  
Data Analysis ◽  
Biomedical Research ◽  
Mass Spectrometry Imaging ◽  
Three Dimensional ◽  
Sample Volume ◽  
Biological Processes ◽  
Full Sample ◽  
Single Slice ◽  
Molecular Complexity

Abstract Mass spectrometry imaging (MSI) enables the visualization of molecular distributions on complex surfaces. It has been extensively used in the field of biomedical research to investigate healthy and diseased tissues. Most of the MSI studies are conducted in a 2D fashion where only a single slice of the full sample volume is investigated. However, biological processes occur within a tissue volume and would ideally be investigated as a whole to gain a more comprehensive understanding of the spatial and molecular complexity of biological samples such as tissues and cells. Mass spectrometry imaging has therefore been expanded to the 3D realm whereby molecular distributions within a 3D sample can be visualized. The benefit of investigating volumetric data has led to a quick rise in the application of single-sample 3D-MSI investigations. Several experimental and data analysis aspects need to be considered to perform successful 3D-MSI studies. In this review, we discuss these aspects as well as ongoing developments that enable 3D-MSI to be routinely applied to multi-sample studies.

P3-071: IN VIVO STABLE ISOTOPE LABELING AND QUANTITATIVE MASS SPECTROMETRY IMAGING OF Aβ PLAQUE DEPOSITION AND TAUOPATHY IN HUMAN AD BRAIN

2006 ◽  
Vol 14 (7S_Part_20) ◽  
pp. P1091-P1092
Author(s):  
Norelle C. Wildburger ◽  
Greg S. Day ◽  
Wendy Sigurdson ◽  
Melissa Sullivan ◽  
Amanda Peters ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Stable Isotope ◽  
Mass Spectrometry Imaging ◽  
Isotope Labeling ◽  
Stable Isotope Labeling ◽  
Quantitative Mass Spectrometry ◽  
Plaque Deposition
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