Fabrication of Silk Fibroin/P(LLA-CL) Aligned Nanofibrous Scaffolds for Nerve Tissue Engineering

2012 ◽  
Vol 298 (5) ◽  
pp. 565-574 ◽  
Author(s):  
Kuihua Zhang ◽  
Wu Jinglei ◽  
Chen Huang ◽  
Xiumei Mo
Keyword(s):  
Tissue Engineering ◽  
Silk Fibroin ◽  
Nerve Tissue ◽  
Nanofibrous Scaffolds ◽  
Nerve Tissue Engineering

In Vitro Evaluation of the Growth and Migration of Schwann Cells on Electrospun Silk Fibroin Nanofibers

2011 ◽  
Vol 175-176 ◽  
pp. 220-223 ◽  
Author(s):  
Ai Jun Hu ◽  
Bao Qi Zuo ◽  
Feng Zhang ◽  
Qing Lan ◽  
Huan Xiang Zhang
Keyword(s):  
Tissue Engineering ◽  
Peripheral Nerve ◽  
Nerve Regeneration ◽  
Schwann Cells ◽  
Silk Fibroin ◽  
Nerve Tissue ◽  
Nerve Tissue Engineering ◽  
And Migration ◽  
Silk Fibroin Nanofibers

Schwann cells (SCs) are primary structural and functional cells in peripheral nervous system and play a crucial role in peripheral nerve regeneration. Current challenge in peripheral nerve tissue engineering is to produce an implantable scaffold capable of bridging long nerve gaps and assist Scs in directing the growth of regenerating axons in nerve injury recovery. Electrospun silk fibroin nanofibers, fabricated for the cell culture in vitro, can provide such experiment support. Silk fibroin scaffolds (SFS) were fabricated with formic acid (FA), and the average fiber diameter was 305 ± 24 nm. The data from microscopic, immunohistochemical and scanning electron micrograph confirmed that the scaffold was beneficial to the adherence, proliferation and migration of SCs without exerting any significant cytotoxic effects on their phenotype. Thus, providing an experimental foundation accelerated the formation of bands of Bünger to enhance nerve regeneration. 305 nm SFS could be a candidate material for nerve tissue engineering.

Electrical Stimulation of Nerve Cells Using Conductive Nanofibrous Scaffolds for Nerve Tissue Engineering

2009 ◽  
Vol 15 (11) ◽  
pp. 3605-3619 ◽  
Author(s):  
Laleh Ghasemi-Mobarakeh ◽  
Molamma P. Prabhakaran ◽  
Mohammad Morshed ◽  
Mohammad Hossein Nasr-Esfahani ◽  
Seeram Ramakrishna
Keyword(s):  
Tissue Engineering ◽  
Electrical Stimulation ◽  
Nerve Cells ◽  
Nerve Tissue ◽  
Nanofibrous Scaffolds ◽  
Nerve Tissue Engineering ◽  
Stimulation Of Nerve ◽  
Stimulation Of

Electrospun poly(ɛ-caprolactone)/gelatin nanofibrous scaffolds for nerve tissue engineering

Biomaterials ◽  
2008 ◽  
Vol 29 (34) ◽  
pp. 4532-4539 ◽  
Author(s):  
Laleh Ghasemi-Mobarakeh ◽  
Molamma P. Prabhakaran ◽  
Mohammad Morshed ◽  
Mohammad-Hossein Nasr-Esfahani ◽  
Seeram Ramakrishna
Keyword(s):  
Tissue Engineering ◽  
Nerve Tissue ◽  
Nanofibrous Scaffolds ◽  
Nerve Tissue Engineering

Surface modified electrospun nanofibrous scaffolds for nerve tissue engineering

2008 ◽  
Vol 19 (45) ◽  
pp. 455102 ◽  
Author(s):  
Molamma P Prabhakaran ◽  
J Venugopal ◽  
Casey K Chan ◽  
S Ramakrishna
Keyword(s):  
Tissue Engineering ◽  
Nerve Tissue ◽  
Nanofibrous Scaffolds ◽  
Nerve Tissue Engineering ◽  
Surface Modified

scholarly journals Fabrication and evaluation of porous and conductive nanofibrous scaffolds for nerve tissue engineering

2021 ◽  
Vol 32 (4) ◽  
Author(s):  
Yasaman Pooshidani ◽  
Nastaran Zoghi ◽  
Mina Rajabi ◽  
Masoumeh Haghbin Nazarpak ◽  
Zahra Hassannejad
Keyword(s):  
Tissue Engineering ◽  
Nerve Repair ◽  
Average Diameter ◽  
Nerve Tissue ◽  
Neural Cells ◽  
Implantable Medical Devices ◽  
Angle Measurements ◽  
Nanofibrous Scaffolds ◽  
Nerve Tissue Engineering ◽  
Controlled Porosity

AbstractPeripheral nerve repair is still one of the major clinical challenges which has received a great deal of attention. Nerve tissue engineering is a novel treatment approach that provides a permissive environment for neural cells to overcome the constraints of repair. Conductivity and interconnected porosity are two required characteristics for a scaffold to be effective in nerve regeneration. In this study, we aimed to fabricate a conductive scaffold with controlled porosity using polycaprolactone (PCL) and chitosan (Chit), FDA approved materials for the use in implantable medical devices. A novel method of using tetrakis (hydroxymethyl) phosphonium chloride (THPC) and formaldehyde was applied for in situ synthesis of gold nanoparticles (AuNPs) on the scaffolds. In order to achieve desirable porosity, different percentage of polyethylene oxide (PEO) was used as sacrificial fiber. Fourier transform infrared spectroscopy (FTIR) and field emission scanning electron microscopy (FE-SEM) results demonstrated the complete removing of PEO from the scaffolds after washing and construction of interconnected porosities, respectively. Elemental and electrical analysis revealed the successful synthesis of AuNPs with uniform distribution and small average diameter on the PCL/Chit scaffold. Contact angle measurements showed the effect of porosity on hydrophilic properties of the scaffolds, where the porosity of 75–80% remarkably improved surface hydrophilicity. Finally, the effect of conductive nanofibrous scaffold on Schwann cells morphology and vaibility was investigated using FE-SEM and MTT assay, respectively. The results showed that these conductive scaffolds had no cytotoxic effect and support the spindle-shaped morphology of cells with elongated process which are typical of Schwann cell cultures.

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