scholarly journals Strontium-Substituted Hydroxyapatite-Gelatin Biomimetic Scaffolds Modulate Bone Cell Response

2018 ◽  
Vol 18 (7) ◽  
pp. 1800096 ◽  
Author(s):  
Silvia Panzavolta ◽  
Paola Torricelli ◽  
Sonia Casolari ◽  
Annapaola Parrilli ◽  
Milena Fini ◽  
...  
Keyword(s):  
Cell Response ◽  
Bone Cell ◽  
Biomimetic Scaffolds

scholarly journals Nanostructure of bioactive glass affects bone cell attachment via protein restructuring upon adsorption

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Ukrit Thamma ◽  
Tia J. Kowal ◽  
Matthias M. Falk ◽  
Himanshu Jain
Keyword(s):  
Bioactive Glass ◽  
Cell Response ◽  
Interfacial Layer ◽  
Cell Attachment ◽  
Bone Cell ◽  
Nano Structure ◽  
Rgd Sequence ◽  
Engineered Tissue ◽  
Α Helix ◽  
Β Sheet

AbstractThe nanostructure of engineered bioscaffolds has a profound impact on cell response, yet its understanding remains incomplete as cells interact with a highly complex interfacial layer rather than the material itself. For bioactive glass scaffolds, this layer comprises of silica gel, hydroxyapatite (HA)/carbonated hydroxyapatite (CHA), and absorbed proteins—all in varying micro/nano structure, composition, and concentration. Here, we examined the response of MC3T3-E1 pre-osteoblast cells to 30 mol% CaO–70 mol% SiO2 porous bioactive glass monoliths that differed only in nanopore size (6–44 nm) yet resulted in the formation of HA/CHA layers with significantly different microstructures. We report that cell response, as quantified by cell attachment and morphology, does not correlate with nanopore size, nor HA/CHO layer micro/nano morphology, or absorbed protein amount (bovine serum albumin, BSA), but with BSA’s secondary conformation as indicated by its β-sheet/α-helix ratio. Our results suggest that the β-sheet structure in BSA interacts electrostatically with the HA/CHA interfacial layer and activates the RGD sequence of absorbed adhesion proteins, such as fibronectin and vitronectin, thus significantly enhancing the attachment of cells. These findings provide new insight into the interaction of cells with the scaffolds’ interfacial layer, which is vital for the continued development of engineered tissue scaffolds.

scholarly journals Bone Cell Response to Physical Activity

2016 ◽  
Vol 4 (3) ◽  
Author(s):  
Díaz Curiel M ◽  
Sierra Poyatos R
Keyword(s):  
Physical Activity ◽  
Cell Response ◽  
Bone Cell

In Vitro Bone-Cell Response to a Capacitively Coupled Electrical Field

1992 ◽  
Vol &NA; (285) ◽  
pp. 255???262 ◽  
Author(s):  
CARL T. BRIGHTON ◽  
ENYI OKEREKE ◽  
SOLOMON R. POLLACK ◽  
CHARLES c. CLARK
Keyword(s):  
Cell Response ◽  
Bone Cell ◽  
Electrical Field ◽  
Capacitively Coupled

scholarly journals In Vitro Bone Cell Response to Tensile Mechanical Solicitations: Is There an Optimal Protocol?

2018 ◽  
Vol 14 (1) ◽  
pp. 1800358
Author(s):  
Timothée Baudequin ◽  
Cécile Legallais ◽  
Fahmi Bedoui
Keyword(s):  
Cell Response ◽  
Bone Cell ◽  
Optimal Protocol

scholarly journals Human foetal osteoblastic cell response to polymer-demixed nanotopographic interfaces

2005 ◽  
Vol 2 (2) ◽  
pp. 97-108 ◽  
Author(s):  
Jung Yul Lim ◽  
Joshua C Hansen ◽  
Christopher A Siedlecki ◽  
James Runt ◽  
Henry J Donahue
Keyword(s):  
Cell Response ◽  
Cell Function ◽  
Early Stage ◽  
Control Cell ◽  
Bone Cell ◽  
Cell Phenotype ◽  
Osteoblastic Cell ◽  
Bone Cell Differentiation ◽  
Biomaterial Interfaces ◽  
Cells Cultured

Nanoscale cell–substratum interactions are of significant interest in various biomedical applications. We investigated human foetal osteoblastic cell response to randomly distributed nanoisland topography with varying heights (11, 38 and 85 nm) produced by a polystyrene (PS)/polybromostyrene polymer-demixing technique. Cells displayed island-conforming lamellipodia spreading, and filopodia projections appeared to play a role in sensing the nanotopography. Cells cultured on 11 nm high islands displayed significantly enhanced cell spreading and larger cell dimensions than cells on larger nanoislands or flat PS control, on which cells often displayed a stellate shape. Development of signal transmitting structures such as focal adhesive vinculin protein and cytoskeletal actin stress fibres was more pronounced, as was their colocalization, in cells cultured on smaller nanoisland surfaces. Cell adhesion and proliferation were greater with decreasing island height. Alkaline phosphatase (AP) activity, an early stage marker of bone cell differentiation, also exhibited nanotopography dependence, i.e. higher AP activity on 11 nm islands compared with that on larger islands or flat PS. Therefore, randomly distributed island topography with varying nanoscale heights not only affect adhesion-related cell behaviour but also bone cell phenotype. Our results suggest that modulation of nanoscale topography may be exploited to control cell function at cell–biomaterial interfaces.

Plasma-Activated Tropoelastin Functionalization of Zirconium for Improved Bone Cell Response

2016 ◽  
Vol 2 (4) ◽  
pp. 662-676 ◽  
Author(s):  
Giselle C. Yeo ◽  
Miguel Santos ◽  
Alexey Kondyurin ◽  
Jana Liskova ◽  
Anthony S. Weiss ◽  
...  
Keyword(s):  
Cell Response ◽  
Bone Cell

Highly Bioactive 8 nm Hydrothermal TiO2 Nanotubes Elicit Enhanced Bone Cell Response

2011 ◽  
Vol 13 (3) ◽  
pp. B88-B94 ◽  
Author(s):  
Karla S. Brammer ◽  
Hyunsu Kim ◽  
Kunbae Noh ◽  
Mariana Loya ◽  
Christine J. Frandsen ◽  
...  
Keyword(s):  
Cell Response ◽  
Tio2 Nanotubes ◽  
Bone Cell

Surface nitridation improves bone cell response to melt-derived bioactive silicate/borosilicate glass composite scaffolds

2016 ◽  
Vol 29 ◽  
pp. 424-434 ◽  
Author(s):  
Felipe Orgaz ◽  
Alexandra Dzika ◽  
Olga Szycht ◽  
Daniel Amat ◽  
Flora Barba ◽  
...  
Keyword(s):  
Borosilicate Glass ◽  
Cell Response ◽  
Bone Cell ◽  
Glass Composite ◽  
Composite Scaffolds
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