scholarly journals Tuning the Buffering Capacity of Polyethylenimine with Glycerol Molecules for Efficient Gene Delivery: Staying In or Out of the Endosomes

2015 ◽  
Vol 15 (5) ◽  
pp. 622-635 ◽  
Author(s):  
Bijay Singh ◽  
Sushila Maharjan ◽  
Tae-Eun Park ◽  
Tao Jiang ◽  
Sang-Kee Kang ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Buffering Capacity ◽  
Efficient Gene

A block copolymer containing PEG and histamine-like segments: well-defined functions for gene delivery

2016 ◽  
Vol 40 (8) ◽  
pp. 7222-7228 ◽  
Author(s):  
Junbo Li ◽  
Jianlong Zhao ◽  
Jiayu Gao ◽  
Ju Liang ◽  
Wenlan Wu ◽  
...  
Keyword(s):  
Block Copolymer ◽  
Gene Delivery ◽  
Colloidal Stability ◽  
Gene Transfection ◽  
Buffering Capacity ◽  
Dna Condensation ◽  
Lower Toxicity ◽  
Efficient Gene

PEG-b-PAMPImB with well-defined functions, including high colloidal stability, effective DNA condensation and buffering capacity, self-assembles with pDNA for lower toxicity and higher efficient gene transfection.

scholarly journals Highly Osmotic Oxidized Sucrose-Crosslinked Polyethylenimine for Gene Delivery Systems

Pharmaceutics ◽  
2021 ◽  
Vol 13 (1) ◽  
pp. 87
Author(s):  
Jaehong Park ◽  
Kyusik Kim ◽  
Sohee Jeong ◽  
Migyeom Lee ◽  
Tae-il Kim
Keyword(s):  
Gene Delivery ◽  
Reductive Amination ◽  
Transfection Efficiency ◽  
Delivery Systems ◽  
Buffering Capacity ◽  
Serum Stability ◽  
High Osmolality ◽  
Cox 2 ◽  
Serum Free Conditions ◽  
Positively Charged

In this work, highly osmotic oxidized sucrose-crosslinked polyethylenimine (SP2K) polymers were developed for gene delivery systems, and the transfection mechanism is examined. First, periodate-oxidized sucrose and polyethylenimine 2K (PEI2K) were crosslinked with various feed ratios via reductive amination. The synthesis was confirmed by 1H NMR and FTIR. The synthesized SP2K polymers could form positively charged (~40 mV zeta-potential) and nano-sized (150–200 nm) spherical polyplexes with plasmid DNA (pDNA). They showed lower cytotoxicity than PEI25K but concentration-dependent cytotoxicity. Among them, SP2K7 and SP2K10 showed higher transfection efficiency than PEI25K in both serum and serum-free conditions, revealing the good serum stability. It was found that SP2K polymers possessed high osmolality and endosome buffering capacity. The transfection experiments with cellular uptake inhibitors suggest that the transfection of SP2K polymers would progress by multiple pathways, including caveolae-mediated endocytosis. It was also thought that caveolae-mediated endocytosis of SP2K polyplexes would be facilitated through cyclooxygenase-2 (COX-2) expression induced by high osmotic pressure of SP2K polymers. Confocal microscopy results also supported that SP2K polyplexes would be internalized into cells via multiple pathways and escape endosomes efficiently via high osmolality and endosome buffering capacity. These results demonstrate the potential of SP2K polymers for gene delivery systems.

Amino acids functionalized dendrimers with nucleus accumulation for efficient gene delivery

2021 ◽  
pp. 120641
Author(s):  
Guoxin Tan ◽  
Jiayang Li ◽  
Dandan Liu ◽  
Hao Pan ◽  
Renfang Zhu ◽  
...  
Keyword(s):  
Amino Acids ◽  
Gene Delivery ◽  
Efficient Gene

scholarly journals Carbon Dot Nanoparticles: Exploring the Potential Use for Gene Delivery in Ophthalmic Diseases

Nanomaterials ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 935
Author(s):  
Manas R. Biswal ◽  
Sofia Bhatia
Keyword(s):  
Gene Delivery ◽  
Therapeutic Option ◽  
Retinal Dystrophy ◽  
Retinal Cells ◽  
Adeno Associated Virus ◽  
Inherited Retinal Dystrophies ◽  
Retinal Dystrophies ◽  
Efficient Gene ◽  
New Type ◽  
Viral Nanoparticles

Ocular gene therapy offers significant potential for preventing retinal dystrophy in patients with inherited retinal dystrophies (IRD). Adeno-associated virus (AAV) based gene transfer is the most common and successful gene delivery approach to the eye. These days, many studies are using non-viral nanoparticles (NPs) as an alternative therapeutic option because of their unique properties and biocompatibility. Here, we discuss the potential of carbon dots (CDs), a new type of nanocarrier for gene delivery to the retinal cells. The unique physicochemical properties of CDs (such as optical, electronic, and catalytic) make them suitable for biosensing, imaging, drug, and gene delivery applications. Efficient gene delivery to the retinal cells using CDs depends on various factors, such as photoluminescence, quantum yield, biocompatibility, size, and shape. In this review, we focused on different approaches used to synthesize CDs, classify CDs, various pathways for the intake of gene-loaded carbon nanoparticles inside the cell, and multiple studies that worked on transferring nucleic acid in the eye using CDs.

Polyethylenimine-poly(amidoamine) dendrimer modified with l-arginines as an efficient gene delivery vector

2015 ◽  
Vol 23 (8) ◽  
pp. 726-733 ◽  
Author(s):  
Nan Young Ahn ◽  
Tae-Hun Kim ◽  
Su Jeong Song ◽  
Jeong-Mi Moon ◽  
Tai Hwan Ha ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Gene Delivery Vector ◽  
Delivery Vector ◽  
Efficient Gene

scholarly journals Efficient gene delivery and silencing of mouse and human pancreatic islets

2010 ◽  
Vol 10 (1) ◽  
pp. 28 ◽  
Author(s):  
Bruno Lefebvre ◽  
Brigitte Vandewalle ◽  
Justine Longue ◽  
Ericka Moerman ◽  
Bruno Lukowiak ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Pancreatic Islets ◽  
Human Pancreatic Islets ◽  
Efficient Gene

Efficient gene delivery using chitosan–polyethylenimine hybrid systems

2008 ◽  
Vol 3 (2) ◽  
pp. 025013 ◽  
Author(s):  
Hu-Lin Jiang ◽  
Tae-Hee Kim ◽  
You-Kyoung Kim ◽  
In-Young Park ◽  
Myung-Haing Cho ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Hybrid Systems ◽  
Efficient Gene
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