Low Charge Density Cationic Polymers for Gene Delivery: Exploring the Influence of Structural Elements on in vitro Transfection

2012 ◽  
Vol 12 (6) ◽  
pp. 840-848 ◽  
Author(s):  
Jonas Mindemark ◽  
Yasuhiko Tabata ◽  
Tim Bowden
Keyword(s):  
Gene Delivery ◽  
Charge Density ◽  
Structural Elements ◽  
Cationic Polymers ◽  
Low Charge ◽  
In Vitro Transfection

Brushlike Cationic Polymers with Low Charge Density for Gene Delivery

2017 ◽  
Vol 19 (5) ◽  
pp. 1410-1415 ◽  
Author(s):  
Jonathan O’Keeffe Ahern ◽  
Sigen A ◽  
Dezhong Zhou ◽  
Yongsheng Gao ◽  
Jing Lyu ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Charge Density ◽  
Cationic Polymers ◽  
Low Charge

Cationic long-chain hyperbranched poly(ethylene glycol)s with low charge density for gene delivery

2013 ◽  
Vol 4 (2) ◽  
pp. 393-401 ◽  
Author(s):  
Chunlai Tu ◽  
Nan Li ◽  
Lijuan Zhu ◽  
Linzhu Zhou ◽  
Yue Su ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Ethylene Glycol ◽  
Charge Density ◽  
Poly Ethylene Glycol ◽  
Hyperbranched Poly ◽  
Poly Ethylene ◽  
Low Charge ◽  
Long Chain

scholarly journals Exploitation of De Novo Helper-Lipids for Effective Gene Delivery.

2009 ◽  
Vol 11 (4) ◽  
pp. 56 ◽  
Author(s):  
Tomoaki Kurosaki ◽  
Takashi Kitahara ◽  
Mugen Teshima ◽  
Koyo Nishida ◽  
Junzo Nakamura ◽  
...  
Keyword(s):  
Gene Delivery ◽  
De Novo ◽  
Transfection Efficiency ◽  
The Other ◽  
Penetration Enhancers ◽  
Cell Toxicity ◽  
In Vivo Transfection ◽  
In Vitro Transfection

Purpose: In gene delivery, a fusogenic lipid such as dioleyl phosphatidylethanolamine (DOPE) which is a component of cationic liposomal vector is important factor for effective transfection efficiency. We investigated the effect of penetration enhancers as alternative helper-lipids to DOPE. Methods: Transdermal penetraion enhancers such as N-lauroylsarcosine (LS), (R)-(+)-limonene (LM), vitamin E (VE), and phosphatidyl choline from eggs (EggPC) were used in this experiments as helper-lipids with N-[1-(2, 3-dioleyloxy) propyl]-N, N, N-trimethlylammonium chloride (DOTMA) and cholesterol (CHOL). We examined in vitro transfection efficiency, cytotoxicity, hematotoxicity, and in vivo transfection efficiency of plasmid DNA/cationic liposomes complexes. Results: In transfection experiments in vitro, the cationic lipoplexes containing LS had highest transfection efficiency among the other lipoplexes independently of FBS. Furthermore, the lipoplexes containing LS had lowest cell toxicity among the other lipoplexes in the presence of FBS. As the results of erythrocytes interaction experiment, DOTMA/LS/CHOL, DOTMA/VE/CHOL, and DOTMA/EggPC/CHOL lipoplexes showed extremely lower hematotoxicity. On the basis of these results, the in vivo transfection efficiencies of the lipoplexes were examined. The lipoplexes containing LS had the highest transfection activity among the other lipoplexes. Conclusion: In conclusion, several transdermal penetration enhancers are available for alternative helper-lipids to DOPE in cationic liposomal vectors. Among them, DOTMA/LS/CHOL lipoplexes showed superior characteristics in in vitro transfection efficiency, cell toxicity, hematotoxicity, and in vivo transfection efficiency.

“Intelligent” nanoassembly for gene delivery: In vitro transfection and the possible mechanism

2010 ◽  
Vol 383 (1-2) ◽  
pp. 271-276 ◽  
Author(s):  
Zhenghong Xu ◽  
Lingli Chen ◽  
Zhiwen Zhang ◽  
Wangwen Gu ◽  
Yaping Li
Keyword(s):  
Gene Delivery ◽  
In Vitro Transfection

A novel chitosan oligosaccharide–stearic acid micelles for gene delivery: Properties and in vitro transfection studies

2006 ◽  
Vol 315 (1-2) ◽  
pp. 158-166 ◽  
Author(s):  
Fu-Qiang Hu ◽  
Meng-Dan Zhao ◽  
Hong Yuan ◽  
Jian You ◽  
Yong-Zhong Du ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Stearic Acid ◽  
Chitosan Oligosaccharide ◽  
In Vitro Transfection

Low Molecular Weight Polyethylenimine GraftedN-Maleated Chitosan for Gene Delivery: Properties and In Vitro Transfection Studies

2008 ◽  
Vol 9 (10) ◽  
pp. 2594-2600 ◽  
Author(s):  
Bo Lu ◽  
Xiao-Ding Xu ◽  
Xian-Zheng Zhang ◽  
Si-Xue Cheng ◽  
Ren-Xi Zhuo
Keyword(s):  
Molecular Weight ◽  
Gene Delivery ◽  
Low Molecular Weight ◽  
In Vitro Transfection
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