Chitosan Grafted with Phosphorylcholine and Macrocyclic Polyamine as an Effective Gene Delivery Vector: Preparation, Characterization and In Vitro Transfection

2015 ◽  
Vol 15 (7) ◽  
pp. 912-926 ◽  
Author(s):  
Ling Li ◽  
Fangfang Zhao ◽  
Baojing Zhao ◽  
Jin Zhang ◽  
Chao Li ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Macrocyclic Polyamine ◽  
Gene Delivery Vector ◽  
Delivery Vector ◽  
In Vitro Transfection

Exosome as a Natural Gene Delivery Vector for Cancer Treatment

2020 ◽  
Vol 20 (11) ◽  
pp. 821-830
Author(s):  
Prasad Pofali ◽  
Adrita Mondal ◽  
Vaishali Londhe
Keyword(s):  
Gene Therapy ◽  
Gene Delivery ◽  
Nucleic Acids ◽  
Cancer Treatment ◽  
Intestinal Barrier ◽  
Gene Carrier ◽  
Gene Delivery Vector ◽  
Delivery Vector

Background: Current gene therapy vectors such as viral, non-viral, and bacterial vectors, which are used for cancer treatment, but there are certain safety concerns and stability issues of these conventional vectors. Exosomes are the vesicles of size 40-100 nm secreted from multivesicular bodies into the extracellular environment by most of the cell types in-vivo and in-vitro. As a natural nanocarrier, exosomes are immunologically inert, biocompatible, and can cross biological barriers like the blood-brain barrier, intestinal barrier, and placental barrier. Objective: This review focusses on the role of exosome as a carrier to efficiently deliver a gene for cancer treatment and diagnosis. The methods for loading of nucleic acids onto the exosomes, advantages of exosomes as a smart intercellular shuttle for gene delivery and therapeutic applications as a gene delivery vector for siRNA, miRNA and Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and also the limitations of exosomes as a gene carrier are all reviewed in this article. Methods: Mostly, electroporation and chemical transfection are used to prepare gene loaded exosomes. Results: Exosome-mediated delivery is highly promising and advantageous in comparison to the current delivery methods for systemic gene therapy. Targeted exosomes, loaded with therapeutic nucleic acids, can efficiently promote the reduction of tumor proliferation without any adverse effects. Conclusion: In the near future, exosomes can become an efficient gene carrier for delivery and a biomarker for the diagnosis and treatment of cancer.

In Vitro Design and Characterization of the Nonviral Gene Delivery Vector Iopamidol, Protamine, Ethiodized Oil Reagent

2011 ◽  
Vol 22 (10) ◽  
pp. 1457-1463.e2 ◽  
Author(s):  
Luke J. Higgins ◽  
Gloria L. Hwang ◽  
Jarrett Rosenberg ◽  
Regina H. Katzenberg ◽  
Nishita Kothary ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Nonviral Gene Delivery ◽  
Gene Delivery Vector ◽  
Delivery Vector ◽  
Ethiodized Oil

scholarly journals Synthesis of a novel PEGDGA-coated hPAMAM complex as an efficient and biocompatible gene delivery vector: an in vitro and in vivo study

Drug Delivery ◽  
2016 ◽  
Vol 23 (8) ◽  
pp. 2956-2969 ◽  
Author(s):  
Mohammad Hemmati ◽  
Farhood Najafi ◽  
Reza Shirkoohi ◽  
Hamid Reza Moghimi ◽  
Amir Zarebkohan ◽  
...  
Keyword(s):  
Gene Delivery ◽  
In Vivo Study ◽  
Gene Delivery Vector ◽  
Delivery Vector
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