scholarly journals Sidestream dark field videomicroscopy for evaluating liver microcirculation in vivo

2017 ◽  
Vol 23 (4) ◽  
pp. 425-426
Author(s):  
Stephen J. Wigmore ◽  
Gabriel C. Oniscu
Keyword(s):  
Dark Field ◽  
Sidestream Dark Field ◽  
Liver Microcirculation

scholarly journals Alterations of cerebral microcirculation in peritumoral edema: feasibility of in vivo sidestream dark-field imaging in intracranial meningiomas

2020 ◽  
Vol 2 (1) ◽  
Author(s):  
Moncef Berhouma ◽  
Thiebaud Picart ◽  
Chloe Dumot ◽  
Isabelle Pelissou-Guyotat ◽  
David Meyronet ◽  
...  
Keyword(s):  
Mass Effect ◽  
Dark Field ◽  
Sidestream Dark Field ◽  
Reference Pattern ◽  
Who Grade ◽  
Perioperative Morbidity ◽  
Sdf Imaging ◽  
Intracranial Meningiomas ◽  
Normal Reference

Abstract Background Intracranial meningiomas display a variable amount of peritumoral brain edema (PTBE), which can significantly impact perioperative morbidity. The role of microcirculatory disturbances in the pathogenesis of PTBE is still debated. The aim of this study was to microscopically demonstrate and intraoperatively quantify, for the first time, the alterations to microcirculation in PTBE using sidestream dark-field (SDF) imaging. Methods Adult patients with WHO grade I meningiomas were recruited over a 9-month period and divided into 2 groups depending on the absence (NE group) or the presence (E group) of PTBE. In vivo intraoperative microcirculation imaging was performed in the peritumoral area before and after microsurgical resection. Results Six patients were included in the NE group and 6 in the E group. At the baseline in the NE group, there was a minor decrease in microcirculatory parameters compared to normal reference values, which was probably due to the mass effect. In contrast, microcirculatory parameters in the E group were significantly altered, affecting both vessel density and blood flow values, with a drop of approximately 50% of normal values. Surgical resection resulted in a quasi-normalization of microcirculation parameters in the NE group, whereas in the E group, even if all parameters statistically significantly improved, post-resection values remained considerably inferior to those of the normal reference pattern. Conclusion Our study confirmed significant alterations of microcirculatory parameters in PTBE in meningiomas. Further in vivo SDF imaging studies may explore the possible correlation between the severity of these microcirculatory alterations and the postoperative neurological outcome.

scholarly journals In situ assessment of the liver microcirculation in mechanically ventilated rats using sidestream dark-field imaging

2009 ◽  
pp. 49-55 ◽  
Author(s):  
V Černý ◽  
Z Turek ◽  
R Pařízková
Keyword(s):  
Animal Studies ◽  
Dark Field ◽  
Target Tissue ◽  
Sidestream Dark Field ◽  
Hepatic Microcirculation ◽  
Midline Laparotomy ◽  
Sdf Imaging ◽  
Liver Microcirculation ◽  
Dark Field Imaging

Assessment of hepatic microcirculation by on-line visualization has been impossible for a long time. Sidestream dark-field (SDF) imaging is a relatively new method allowing direct visualization of both mucosal microcirculation and surface layers microcirculation of solid organs using hand-held probe for direct contact with target tissue. The aim of this study was to evaluate the feasibility of studying the rat hepatic microcirculation in situ by SDF imaging. The liver lobes were left in situ, and images were obtained using SDF imaging on the surface of the liver via upper midline laparotomy. Images were captured intermittently during 10-sec apnoea and recorded. The microvascular parameters were compared with previous validation studies. Clear high contrast SDF images were successfully obtained. Quantitative analysis revealed a mean FSD (functional sinusoidal density) of 402±15 cm/cm2, a sinusoidal diameter of 10.2±0.5 μm and postsinusoidal venular diameter of 33.9±13 μm. SDF imaging is a suitable noninvasive method for accurate quantification of the basic microcirculatory parameters of the liver in situ without a need to exteriorize the liver lobes. This method seems to be applicable in animal studies with possibility to use SDF imaging also intraoperatively, providing unique opportunity to study liver microcirculation during various experimental and clinical settings.

scholarly journals Quantitative laser speckle flowmetry of the in vivo microcirculation using sidestream dark field microscopy

2013 ◽  
Vol 4 (11) ◽  
pp. 2347 ◽  
Author(s):  
Annemarie Nadort ◽  
Rutger G. Woolthuis ◽  
Ton G. van Leeuwen ◽  
Dirk J. Faber
Keyword(s):  
Dark Field ◽  
Laser Speckle ◽  
Sidestream Dark Field ◽  
Dark Field Microscopy

scholarly journals Noninvasive, in vivo assessment of the cervical microcirculation using incident dark field imaging

2021 ◽  
Vol 135 ◽  
pp. 104145
Author(s):  
Yani P. Latul ◽  
Arnoud W. Kastelein ◽  
Patricia W.T. Beemster ◽  
Nienke E. van Trommel ◽  
Can Ince ◽  
...  
Keyword(s):  
Dark Field ◽  
Dark Field Imaging ◽  
Incident Dark Field Imaging ◽  
Field Imaging ◽  
In Vivo Assessment

scholarly journals Correlation of image quality parameters with tube voltage in X-ray dark-field chest radiography: a phantom study

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Andreas P. Sauter ◽  
Jana Andrejewski ◽  
Manuela Frank ◽  
Konstantin Willer ◽  
Julia Herzen ◽  
...  
Keyword(s):  
Image Quality ◽  
Imaging Modality ◽  
Signal Strength ◽  
Dark Field ◽  
Tube Voltage ◽  
X Ray ◽  
Dose Area Product ◽  
Dark Field Imaging ◽  
Field Imaging

AbstractGrating-based X-ray dark-field imaging is a novel imaging modality with enormous technical progress during the last years. It enables the detection of microstructure impairment as in the healthy lung a strong dark-field signal is present due to the high number of air-tissue interfaces. Using the experience from setups for animal imaging, first studies with a human cadaver could be performed recently. Subsequently, the first dark-field scanner for in-vivo chest imaging of humans was developed. In the current study, the optimal tube voltage for dark-field radiography of the thorax in this setup was examined using an anthropomorphic chest phantom. Tube voltages of 50–125 kVp were used while maintaining a constant dose-area-product. The resulting dark-field and attenuation radiographs were evaluated in a reader study as well as objectively in terms of contrast-to-noise ratio and signal strength. We found that the optimum tube voltage for dark-field imaging is 70 kVp as here the most favorable combination of image quality, signal strength, and sharpness is present. At this voltage, a high image quality was perceived in the reader study also for attenuation radiographs, which should be sufficient for routine imaging. The results of this study are fundamental for upcoming patient studies with living humans.

Evaluation of Microvascular Perfusion and Resuscitation after Severe Injury

2015 ◽  
Vol 81 (12) ◽  
pp. 1272-1278 ◽  
Author(s):  
Yann-Leei L. Lee ◽  
Jon D. Simmons ◽  
Mark N. Gillespie ◽  
Diego F. Alvarez ◽  
Richard P. Gonzalez ◽  
...  
Keyword(s):  
Injury Severity ◽  
Capillary Flow ◽  
Severe Trauma ◽  
Dark Field ◽  
Serum Lactate ◽  
Microvascular Perfusion ◽  
Trauma Patients ◽  
Sidestream Dark Field ◽  
Trauma Population ◽  
Severity Scores

Achieving adequate perfusion is a key goal of treatment in severe trauma; however, tissue perfusion has classically been measured by indirect means. Direct visualization of capillary flow has been applied in sepsis, but application of this technology to the trauma population has been limited. The purpose of this investigation was to compare the efficacy of standard indirect measures of perfusion to direct imaging of the sublingual microcirculatory flow during trauma resuscitation. Patients with injury severity scores >15 were serially examined using a handheld sidestream dark-field video microscope. In addition, measurements were also made from healthy volunteers. The De Backer score, a morphometric capillary density score, and total vessel density (TVD) as cumulative vessel area within the image, were calculated using Automated Vascular Analysis (AVA3.0) software. These indices were compared against clinical and laboratory parameters of organ function and systemic metabolic status as well as mortality. Twenty severely injured patients had lower TVD (X = 14.6 ± 0.22 vs 17.66 ± 0.51) and De Backer scores (X = 9.62 ± 0.16 vs 11.55 ± 0.37) compared with healthy controls. These scores best correlated with serum lactate (TVD R2 = 0.525, De Backer R2 = 0.576, P < 0.05). Mean arterial pressure, heart rate, oxygen saturation, pH, bicarbonate, base deficit, hematocrit, and coagulation parameters correlated poorly with both TVD and De Backer score. Direct measurement of sublingual microvascular perfusion is technically feasible in trauma patients, and seems to provide real-time assessment of micro-circulatory perfusion. This study suggests that in severe trauma, many indirect measurements of perfusion do not correlate with microvascular perfusion. However, visualized perfusion deficiencies do reflect a shift toward anaerobic metabolism.

scholarly journals Comparison of Microcirculatory Perfusion in Obese and Non-Obese Patients Undergoing Cardiac Surgery with Cardiopulmonary Bypass

2021 ◽  
Vol 10 (3) ◽  
pp. 469
Author(s):  
Chantal A. Boly ◽  
Margot Venhuizen ◽  
Nicole A. M. Dekker ◽  
Alexander B. A. Vonk ◽  
Christa Boer ◽  
...  
Keyword(s):  
Cardiac Surgery ◽  
Cardiopulmonary Bypass ◽  
Type Ii Diabetes Mellitus ◽  
Dark Field ◽  
Multiple Organ ◽  
Obese Patients ◽  
Sidestream Dark Field ◽  
Microcirculatory Perfusion ◽  
Metabolically Healthy Obese ◽  
Healthy Obese

Obesity is a frequent comorbidity among patients undergoing cardiac surgery with cardiopulmonary bypass (CPB). Cardiac surgery with CPB impairs microcirculatory perfusion, which is associated with multiple organ failure. As microvascular function is frequently compromised in obese patients, we studied whether cardiac surgery with CPB has a more detrimental effect on microcirculatory perfusion in obese patients. Sublingual microcirculatory perfusion was measured with sidestream dark field (SDF) imaging in obese patients (body mass index ≥32 kg/m2; n = 14) without type II diabetes mellitus and in lean patients (BMI 20–25 kg/m2; n = 22) undergoing cardiac surgery with CPB. CPB reduced systolic blood pressure and mean arterial pressure more profoundly in lean compared with obese patients (SBP: 38% vs. 18%; MAP: 11% vs. 8%, p < 0.05), and both restored after weaning from CPB. No differences were present in intraoperative glucose, hematocrit, hemoglobin, lactate, and blood gas values between obese and lean patients. Microcirculatory perfusion did not differ between obese and lean patients the day before surgery. CPB decreased microcirculatory perfusion with 9% in both groups, but this was only significant in lean patients (p < 0.05). Three days following surgery, microcirculatory perfusion was restored in both groups. In conclusion, microcirculatory perfusion was equally disturbed during cardiac surgery with CPB in metabolically healthy obese patients compared to lean patients.

Sign in / Sign up

Export Citation Format

Share Document