Light‐activated photosealing with human amniotic membrane strengthens bowel anastomosis in a hypotensive, trauma‐relevant swine model

2021 ◽  
Author(s):  
Benjamin B. Scott ◽  
Ying Wang ◽  
Ruby C. Wu ◽  
Mark A. Randolph ◽  
Robert W. Redmond
Keyword(s):  
Amniotic Membrane ◽  
Human Amniotic Membrane ◽  
Swine Model ◽  
Bowel Anastomosis

scholarly journals Evaluation of Human Amniotic Membrane as a Wound Dressing for Split-Thickness Skin-Graft Donor Sites

2014 ◽  
Vol 2014 ◽  
pp. 1-12 ◽  
Author(s):  
Denys J. Loeffelbein ◽  
Nils H. Rohleder ◽  
Matthias Eddicks ◽  
Claudia M. Baumann ◽  
Mechthild Stoeckelhuber ◽  
...  
Keyword(s):  
Basement Membrane ◽  
Skin Graft ◽  
Amniotic Membrane ◽  
Wound Dressing ◽  
Membrane Integrity ◽  
Split Thickness Skin Graft ◽  
Human Amniotic Membrane ◽  
Swine Model ◽  
Pu Foam ◽  
Thickness Skin

Human amniotic membrane (HAM) has been used as a biomaterial in various surgical procedures and exceeds some qualities of common materials. We evaluated HAM as wound dressing for split-thickness skin-graft (STSG) donor sites in a swine model (Part A) and a clinical trial (Part B). Part A: STSG donor sites in 4 piglets were treated with HAM or a clinically used conventional polyurethane (PU) foil (n=8each). Biopsies were taken on days 5, 7, 10, 20, 40, and 60 and investigated immunohistochemically for alpha-smooth muscle actin (αSMA: wound contraction marker), von Willebrand factor (vWF: angiogenesis), Ki-67 (cell proliferation), and laminin (basement membrane integrity). Part B: STSG donor sites in 45 adult patients (16 female/29 male) were treated with HAM covered by PU foam, solely by PU foam, or PU foil/paraffin gauze (n=15each). Part A revealed no difference in the rate of wound closure between groups. HAM showed improved esthetic results and inhibitory effects on cicatrization. Angioneogenesis was reduced, and basement membrane formation was accelerated in HAM group. Part B: no difference in re-epithelialization/infection rate was found. HAM caused less ichor exudation and less pruritus. HAM has no relevant advantage over conventional dressings but might be a cost-effective alternative.

Effect of viable cryopreserved human amniotic membrane sutured to cornea on course of experimental bacterial keratitis

2015 ◽  
Vol 57 (5) ◽  
pp. 35-41
Author(s):  
K. Sereda ◽  
◽  
G. Drozhzhina ◽  
T. Gaidamaka ◽  
V. Vit ◽  
...  
Keyword(s):  
Amniotic Membrane ◽  
Human Amniotic Membrane ◽  
Bacterial Keratitis

scholarly journals Human Amniotic Membrane as a Matrix for Endothelial Differentiation of VEGF-Treated Dental Stem Cells

2019 ◽  
Vol 12 (6) ◽  
pp. 599-613 ◽  
Author(s):  
Siti Nurnasihah Md Hashim ◽  
Muhammad Fuad Hilmi Yusof ◽  
Wafa’ Zahari ◽  
Hamshawagini Chandra ◽  
Khairul Bariah Ahmad Amin Noordin ◽  
...  
Keyword(s):  
Stem Cells ◽  
Amniotic Membrane ◽  
Endothelial Differentiation ◽  
Human Amniotic Membrane ◽  
Dental Stem Cells

Human amniotic membrane as a delivery vehicle for stem cell-based therapies

Life Sciences ◽  
2021 ◽  
pp. 119157
Author(s):  
Ping Chen ◽  
Minjun Lu ◽  
Tao Wang ◽  
Dongchun Dian ◽  
Yong Zhong ◽  
...  
Keyword(s):  
Stem Cell ◽  
Amniotic Membrane ◽  
Human Amniotic Membrane ◽  
Delivery Vehicle

scholarly journals The Antibacterial Activity of Human Amniotic Membrane against Multidrug-Resistant Bacteria Associated with Urinary Tract Infections: New Insights from Normal and Cancerous Urothelial Models

Biomedicines ◽  
2021 ◽  
Vol 9 (2) ◽  
pp. 218
Author(s):  
Taja Železnik Ramuta ◽  
Larisa Tratnjek ◽  
Aleksandar Janev ◽  
Katja Seme ◽  
Marjanca Starčič Erjavec ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Amniotic Membrane ◽  
Multidrug Resistant ◽  
Basic Knowledge ◽  
Resistant Bacteria ◽  
Human Amniotic Membrane ◽  
Multidrug Resistant Bacteria ◽  
Tract Infections

Urinary tract infections (UTIs) represent a serious global health issue, especially due to emerging multidrug-resistant UTI-causing bacteria. Recently, we showed that the human amniotic membrane (hAM) could be a candidate for treatments and prevention of UPEC and Staphylococcus aureus infections. However, its role against multidrug-resistant bacteria, namely methicillin-resistant S. aureus (MRSA), extended-spectrum beta-lactamases (ESBL) producing Escherichia coli and Klebsiella pneumoniae, vancomycin-resistant Enterococci (VRE), carbapenem-resistant Acinetobacter baumannii, and Pseudomonas aeruginosa has not yet been thoroughly explored. Here, we demonstrate for the first time that the hAM homogenate had antibacterial activity against 7 out of 11 tested multidrug-resistant strains, the greatest effect was on MRSA. Using novel approaches, its activity against MRSA was further evaluated in a complex microenvironment of normal and cancerous urinary bladder urothelia. Even short-term incubation in hAM homogenate significantly decreased the number of bacteria in MRSA-infected urothelial models, while it did not affect the viability, number, and ultrastructure of urothelial cells. The hAM patches had no antibacterial activity against any of the tested strains, which further exposes the importance of the hAM preparation. Our study substantially contributes to basic knowledge on the antibacterial activity of hAM and reveals its potential to be used as an antibacterial agent against multidrug-resistant bacteria.

scholarly journals A Novel Phenotype of Junctional Epidermolysis Bullosa with Transient Skin Fragility and Predominant Ocular Involvement Responsive to Human Amniotic Membrane Eyedrops

Genes ◽  
2021 ◽  
Vol 12 (5) ◽  
pp. 716
Author(s):  
Daniele Castiglia ◽  
Paola Fortugno ◽  
Angelo Giuseppe Condorelli ◽  
Sabina Barresi ◽  
Naomi De Luca ◽  
...  
Keyword(s):  
Amniotic Membrane ◽  
Epidermolysis Bullosa ◽  
Ocular Involvement ◽  
Human Amniotic Membrane ◽  
Vitro Assay ◽  
Junctional Epidermolysis Bullosa ◽  
Mucosal Involvement ◽  
Skin Fragility ◽  
Laminin 332

Junctional epidermolysis bullosa (JEB) is a clinically and genetically heterogeneous skin fragility disorder frequently caused by mutations in genes encoding the epithelial laminin isoform, laminin-332. JEB patients also present mucosal involvement, including painful corneal lesions. Recurrent corneal abrasions may lead to corneal opacities and visual impairment. Current treatments are merely supportive. We report a novel JEB phenotype distinguished by the complete resolution of skin fragility in infancy and persistent ocular involvement with unremitting and painful corneal abrasions. Biallelic LAMB3 mutations c.3052-5C>G and c.3492_3493delCG were identified as the molecular basis for this phenotype, with one mutation being a hypomorphic splice variant that allows residual wild-type laminin-332 production. The reduced laminin-332 level was associated with impaired keratinocyte adhesion. Then, we also investigated the therapeutic power of a human amniotic membrane (AM) eyedrop preparation for corneal lesions. AM were isolated from placenta donors, according to a procedure preserving the AM biological characteristics as a tissue, and confirmed to contain laminin-332. We found that AM eyedrop preparation could restore keratinocyte adhesion in an in vitro assay. Of note, AM eyedrop administration to the patient resulted in long-lasting remission of her ocular manifestations. Our findings suggest that AM eyedrops could represent an effective, non-invasive, simple-to-handle treatment for corneal lesions in patients with JEB and possibly other EB forms.

scholarly journals Case report of non-healing surgical wound treated with dehydrated human amniotic membrane

2015 ◽  
Vol 13 (1) ◽  
Author(s):  
Neil H Riordan ◽  
Ben A George ◽  
Troy B Chandler ◽  
Randall W McKenna
Keyword(s):  
Case Report ◽  
Amniotic Membrane ◽  
Human Amniotic Membrane ◽  
Surgical Wound

Treatment ofPseudomonas aeruginosakeratitis with combined corneal cross-linking and human amniotic membrane transplantation

2013 ◽  
Vol 91 (5) ◽  
pp. e410-e411 ◽  
Author(s):  
Jaakko S. Mattila ◽  
Anna Korsbäck ◽  
Kari Krootila ◽  
Juha M. Holopainen
Keyword(s):  
Amniotic Membrane ◽  
Amniotic Membrane Transplantation ◽  
Cross Linking ◽  
Human Amniotic Membrane
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