The structure of grease via electron microscopy and image analysis

1991 ◽  
Vol 4 (1) ◽  
pp. 35-50 ◽  
Author(s):  
P. J. Shuff ◽  
L. J. Clarke
Keyword(s):  
Electron Microscopy ◽  
Image Analysis

Morphometric analysis of cellular interactions with the endothelium during the development of arterial lesions using Scanning Electron Microscopy and digital image analysis

1987 ◽  
Vol 45 ◽  
pp. 918-919
Author(s):  
M.E. Rosenfeld ◽  
C. Karboski ◽  
M.F. Prescott ◽  
P. Goodwin ◽  
R. Ross
Keyword(s):  
Electron Microscopy ◽  
Image Analysis ◽  
Quantitative Data ◽  
Digital Image Analysis ◽  
Lower Cost ◽  
Matched Control ◽  
Digital Analysis ◽  
Cellular Interactions ◽  
Arterial Lesions ◽  
Scanning Electron

Previous research documenting the chronology of the cellular interactions that occur on or below the surface of the endothelium during the initiation and progression of arterial lesions, primarily consisted of descriptive studies. The recent development of lower cost image analysis hardware and software has facilitated the collection of high resolution quantitative data from microscopic images. In this report we present preliminary quantitative data on the sequence of cellular interactions that occur on the endothelium during the initiation of atherosclerosis or vasculitis utilizing digital analysis of images obtained directly from the scanning electron microscope. Segments of both atherosclerotic and normal arteries were obtained from either diet-induced or endogenously (WHHL) hypercholesterolemic rabbits following 1-4 months duration of hypercholesterolemia and age matched control rabbits. Vasculitis was induced in rats following placement of an endotoxin soaked thread adjacent to the adventitial surface of arteries.

Applications of CCEM to Environmental Health Problems

1985 ◽  
Vol 43 ◽  
pp. 102-103
Author(s):  
R. J. Lee ◽  
J. S. Walker
Keyword(s):  
Electron Microscopy ◽  
Image Analysis ◽  
Environmental Health ◽  
Computer Control ◽  
External Agent ◽  
External Agents ◽  
Computer Controlled ◽  
Primitive State ◽  
Textural Changes ◽  
General Aspects

Electron microscopy (EM), with the advent of computer control and image analysis techniques, is rapidly evolving from an interpretative science into a quantitative technique. Electron microscopy is potentially of value in two general aspects of environmental health: exposure and diagnosis.In diagnosis, electron microscopy is essentially an extension of optical microscopy. The goal is to characterize cellular changes induced by external agents. The external agent could be any foreign material, chemicals, or even stress. The use of electron microscopy as a diagnostic tool is well- developed, but computer-controlled electron microscopy (CCEM) has had only limited impact, mainly because it is fairly new and many institutions lack the resources to acquire the capability. In addition, major contributions to diagnosis will come from CCEM only when image analysis (IA) and processing algorithms are developed which allow the morphological and textural changes recognized by experienced medical practioners to be quantified. The application of IA techniques to compare cellular structure is still in a primitive state.

Electron Microscopy and image analysis of nucleo-protein complexes

1994 ◽  
Vol 52 ◽  
pp. 88-89
Author(s):  
E. H. Egelman ◽  
X. Yu
Keyword(s):  
Electron Microscopy ◽  
Image Analysis ◽  
Normal Cell ◽  
Genetic Recombination ◽  
Protein Complexes ◽  
Chromosomal Rearrangements ◽  
Reca Protein ◽  
E Coli ◽  
Helical Polymer ◽  
Specific Protease

The RecA protein of E. coli has been shown to mediate genetic recombination, regulate its own synthesis, control the expression of other genes, act as a specific protease, form a helical polymer and have an ATPase activity, among other observed properties. The unusual filament formed by the RecA protein on DNA has not previously been shown to exist outside of bacteria. Within this filament, the 36 Å pitch of B-form DNA is extended to about 95 Å, the pitch of the RecA helix. We have now establishedthat similar nucleo-protein complexes are formed by bacteriophage and yeast proteins, and availableevidence suggests that this structure is universal across all of biology, including humans. Thus, understanding the function of the RecA protein will reveal basic mechanisms, in existence inall organisms, that are at the foundation of general genetic recombination and repair.Recombination at this moment is assuming an importance far greater than just pure biology. The association between chromosomal rearrangements and neoplasms has become stronger and stronger, and these rearrangements are most likely products of the recombinatory apparatus of the normal cell. Further, damage to DNA appears to be a major cause of cancer.

scholarly journals Microtubule organization within mitotic spindles revealed by serial block face scanning electron microscopy and image analysis

2017 ◽  
Vol 130 (10) ◽  
pp. 1845-1855 ◽  
Author(s):  
Faye M. Nixon ◽  
Thomas R. Honnor ◽  
Nicholas I. Clarke ◽  
Georgina P. Starling ◽  
Alison J. Beckett ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Image Analysis ◽  
Mitotic Spindles ◽  
Microtubule Organization ◽  
Face Scanning ◽  
Block Face ◽  
Scanning Electron

Measuring Coral Skeletal Architecture Using Image Analysis v1

2021 ◽  
Author(s):  
Rowan Mclachlan ◽  
Ashruti Patel ◽  
Andrea G Grottoli
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Image Analysis ◽  
Scleractinian Coral ◽  
Coral Species ◽  
Energy Materials ◽  
Simple Method ◽  
Coral Skeletons ◽  
Skeletal Structures ◽  
Scanning Electron

Coral morphology is influenced by genetics, the environment, or the interaction of both, and thus is highly variable. This protocol outlines a non-destructive and relatively simple method for measuring Scleractinian coral sub-corallite skeletal structures (such as the septa length, theca thickness, and corallite diameter, etc.) using digital images produced as a result of digital microscopy or from scanning electron microscopy. This method uses X and Y coordinates of points placed onto photomicrographs to automatically calculate the length and/or diameter of a variety of sub-corallite skeletal structures in the Scleractinian coral Porites lobata. However, this protocol can be easily adapted for other coral species - the only difference may be the specific skeletal structures that are measured (for example, not all coral species have a pronounced columella or pali, or even circular corallites). This protocol is adapted from the methods described in Forsman et al. (2015) & Tisthammer et al. (2018). There are 4 steps to this protocol: 1) Removal of Organic Tissue from Coral Skeletons 2) Imaging of Coral Skeletons 3) Photomicrograph Image Analysis 4) Calculation of Corallite Microstructure Size This protocol was written by Dr. Rowan McLachlan and was reviewed by Ashruti Patel and Dr. Andréa Grottoli. Acknowledgments Leica DMS 1000 and Scanning Electron Microscopy photomicrographs used in this protocol were acquired at the Subsurface Energy Materials Characterization and Analysis Laboratory (SEMCAL), School of Earth Sciences at The Ohio State University, Ohio, USA. I would like to thank Dr. Julie Sheets, Dr. Sue Welch, and Dr. David Cole for training me on the use of these instruments.

scholarly journals Structure and Polymorphism of the UL6 Portal Protein of Herpes Simplex Virus Type 1

2004 ◽  
Vol 78 (22) ◽  
pp. 12668-12671 ◽  
Author(s):  
Benes L. Trus ◽  
Naiqian Cheng ◽  
William W. Newcomb ◽  
Fred L. Homa ◽  
Jay C. Brown ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Image Analysis ◽  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Virus Type ◽  
Herpes Simplex Virus Type ◽  
Portal Protein ◽  
Axial Channel ◽  
Simplex Virus

ABSTRACT By electron microscopy and image analysis, we find that baculovirus-expressed UL6 is polymorphic, consisting of rings of 11-, 12-, 13-, and 14-fold symmetry. The 12-mer is likely to be the oligomer incorporated into procapsids: at a resolution of 16 Å, it has an axial channel, peripheral flanges, and fits snugly into a vacant vertex site. Its architecture resembles those of bacteriophage portal/connector proteins.

scholarly journals Defects of copper patina / Vady mìdìných patin

2015 ◽  
Vol 59 (3) ◽  
pp. 87-90 ◽  
Author(s):  
J. Stoulil ◽  
P. Šedá ◽  
M. Anisová ◽  
Z. Fencl ◽  
P. Novák ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Image Analysis ◽  
Compact Surface ◽  
Historical Buildings ◽  
X Ray Diffraction ◽  
X Ray ◽  
Destructive Analysis ◽  
High Degree ◽  
Scanning Electron

Abstract The paper is focused on analyses of dark copper patina defects that were formed on one sheet under the same conditions. Roofs of ten historical buildings were studied by image analysis and samples of two roofs were subjected to more detailed destructive analysis. These samples were studied by means of scanning electron microscopy with energy dispersive X-ray spectroscopy, X-ray diffraction and infrared spectroscopy. Both types of patina are composed of brochantite. Green patinas consisted of a pure brochantite and they had a fl at and compact surface. Conversely, black patina contained a high degree of impurities (ammonia cations, nitrates, silicates) and the surface was rough. The proportion of dark patina was higher in south and east facing surfaces, where washing by rainfall is more difficult.

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