Surface-modified silicone T-tubes for prevention of tracheal stenosis in a rabbit model

2014 ◽  
Vol 125 (6) ◽  
pp. 1465-1471 ◽  
Author(s):  
Jeong-Seok Choi ◽  
Jae-Yol Lim ◽  
In S. Park ◽  
Si Y. Seo ◽  
Yoon K. Joung ◽  
...  
Keyword(s):  
Tracheal Stenosis ◽  
Rabbit Model ◽  
Surface Modified

scholarly journals THREE-DIMENSIONAL NANOCOMPOSITE SCAFFOLDS FOR BONE TISSUE ENGINEERING: FROM DESIGN TO APPLICATION

Nano LIFE ◽  
2012 ◽  
Vol 02 (01) ◽  
pp. 1250005 ◽  
Author(s):  
BIN DUAN ◽  
MIN WANG ◽  
WILLIAM W. LU
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Rabbit Model ◽  
Bone Morphogenetic Protein 2 ◽  
Human Umbilical Cord ◽  
Porous Structures ◽  
Tissue Engineering Scaffolds ◽  
Surface Modified ◽  
Nanocomposite Scaffolds

Selective laser sintering (SLS), a rapid prototyping technology, was investigated for producing bone tissue engineering scaffolds. Completely biodegradable osteoconductive calcium phosphate (Ca-P)/poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) scaffolds were successfully fabricated via SLS using Ca-P/PHBV nanocomposite microspheres. In the SLS manufacturing route, the architecture of tissue engineering scaffolds (pore shape, size, interconnectivity, etc.) can be designed and the sintering process can be optimized for obtaining scaffolds with desirable porous structures and mechanical properties. SLS was also shown to be very effective in producing highly complex porous structures using nanocomposite microspheres. To render SLS-formed Ca-P/PHBV scaffolds osteoinductive, recombinant human bone morphogenetic protein-2 (rhBMP-2) could be loaded onto the scaffolds. For achieving a controlled release of rhBMP-2 from scaffolds, surface modification of Ca-P/PHBV scaffolds by gelatin entrapment and heparin immobilization was needed. The immobilized heparin provided binding affinity for rhBMP-2. Surface modified Ca-P/PHBV nanocomposite scaffolds loaded with rhBMP-2 enhanced the proliferation of human umbilical cord derived mesenchymal stem cells (hUCMSCs) and also their alkaline phosphatase activity. In in vivo experiments using a rabbit model, surface modified Ca-P/PHBV nanocomposite scaffolds loaded with rhBMP-2 promoted ectopic bone formation, exhibiting their osteoinductivity. The strategy of combining advanced scaffold fabrication, nanocomposite material, and controlled growth factor delivery is promising for bone tissue regeneration.

scholarly journals Role of Erythromycin-Regulated Histone Deacetylase-2 in Benign Tracheal Stenosis

2020 ◽  
Vol 2020 ◽  
pp. 1-10
Author(s):  
Zhenjie Huang ◽  
Peng Wei ◽  
Luoman Gan ◽  
Tonghua Zeng ◽  
Caicheng Qin ◽  
...  
Keyword(s):  
Histone Deacetylase ◽  
Tracheal Stenosis ◽  
Type I Collagen ◽  
Rabbit Model ◽  
Immunohistochemical Method ◽  
Type Iii Collagen ◽  
Type I ◽  
Histone Deacetylase 2 ◽  
Budesonide Group

Objective. This study aims to explore the role of erythromycin-regulated histone deacetylase-2 in benign tracheal stenosis. Methods. The rabbit model of tracheal stenosis was established. The rabbits were randomly divided into 8 groups. Histone deacetylase-2 (HDAC2) expression was detected by immunofluorescence. The expression of type I collagen and type III collagen was detected by immunohistochemical method. The expression of TGF-β1, VEGF and IL-8 in serum and alveolar lavage fluid was detected by ELISA. The expression of HDAC2, TGF-β1, VEGF and IL-8 in bronchi of each group was detected by Western blotting method. Results. In Erythromycin (ERY) group, ERY + Budesonide group, ERY + Vorinostat group and ERY + Budesonide + Vorinostat group, the degree of bronchial stenosis was alleviated, and the mucosal epithelium was still slightly proliferated. The effect of ERY combined with other drugs was more obvious. The HDAC2 protein expression increased significantly in ERY group, ERY + Budesonide group and ERY + Budesonide + Vorinostat group and decreased significantly in Vorinostat group, the expression of collagen I and III decreased significantly in ERY group, ERY + Budesonide group and ERY + Budesonide + Vorinostat group (P<0.05). The TGF-β1, IL-8 and VEGF levels decreased significantly in ERY group, ERY + Budesonide group, ERY + Vorinostat group and ERY + Budesonide + Vorinostat group (P<0.05). Conclusions. Erythromycin inhibited inflammation and excessive proliferation of granulation tissue after tracheobronchial mucosal injury by up-regulating the expression of HDAC2, it promoted wound healing and alleviated tracheobronchial stenosis. When combined with budesonide, penicillin and other glucocorticoids and antibiotics, it had a good synergistic effect. However, vorinostat could attenuate erythromycin’s effect by down-regulating the expression of HDAC2. It may have good clinical application prospects in the treatment of tracheal stenosis.

Induction of tracheal stenosis in a rabbit model-endoscopic versus open technique

2011 ◽  
Vol 121 (3) ◽  
pp. 509-514 ◽  
Author(s):  
Matthew K. Steehler ◽  
Hosai N. Hesham ◽  
Benjamin J. Wycherly ◽  
Kevin M. Burke ◽  
Sonya Malekzadeh
Keyword(s):  
Tracheal Stenosis ◽  
Rabbit Model ◽  
Open Technique

Tracheal Stenosis Induction: A Comparison of Open Versus Endoscopic Methods in a Rabbit Model

2009 ◽  
Vol 119 (S1) ◽  
pp. S105-S105
Author(s):  
Benjamin J. Wycherly ◽  
Hosai N. Hesham ◽  
Sonya Malekzadeh ◽  
Matthew K. Steehler ◽  
Kevin M. Burke
Keyword(s):  
Tracheal Stenosis ◽  
Rabbit Model ◽  
Open Versus ◽  
Endoscopic Methods

scholarly journals Vancomycin-Loaded Polycaprolactone Electrospinning Nanofibers Modulate the Airway Interfaces to Restrain Tracheal Stenosis

2021 ◽  
Vol 9 ◽  
Author(s):  
Yanan Zhao ◽  
Chuan Tian ◽  
Kunpeng Wu ◽  
Xueliang Zhou ◽  
Kexing Feng ◽  
...  
Keyword(s):  
Tracheal Stenosis ◽  
Rabbit Model ◽  
Biological Properties ◽  
Covered Stent ◽  
Metallic Stent ◽  
Electrospinning Technique ◽  
Airway Stent ◽  
Expandable Metallic Stent ◽  
Covered Metallic Stent ◽  
Ft Ir

Site-specific release of therapeutics at the infected trachea remains a great challenge in clinic. This work aimed to develop a series of vancomycin (VA)-loaded polycaprolactone (PCL) composite nanofiber films (PVNF-n, n = 0, 1, and 5, respectively) via the electrospinning technique. The physiochemical and biological properties of PVNF-n were evaluated by a series of tests, such as FT-IR, XRD, SEM-EDS, and antibacterial assay. The PVNF-n samples displayed a typical network structure of fibers with random directions. VA was successfully introduced into the PCL nanofibers and could be sustained and released. More importantly, PVNF-5 showed relatively good antibacterial activity against both methicillin-resistant Staphylococcus aureus (MRSA) and Streptococcus pneumoniae (SPn). Thus, PVNF-5 was covered onto the self-expandable metallic stent and then implanted into a New Zealand rabbit model to repair tracheal stenosis. Compared to a metallic stent, a commercial pellosil matrix–covered stent, and a PVNF-0–covered metallic stent, the PVNF-5–covered airway stent showed reduced granulation tissue thickness, collagen density, α-SMA, CD68, TNF-α, IL-1, and IL-6 expression. In conclusion, this work provides an anti-infection film–covered airway stent that in site restrains tracheal stenosis.

Rabbit model of tracheal stenosis induced by prolonged endotracheal intubation using a segmented tube

2015 ◽  
Vol 79 (12) ◽  
pp. 2384-2388 ◽  
Author(s):  
Hyoung Shin Lee ◽  
Sung Won Kim ◽  
Chulho Oak ◽  
Yeh-Chan Ahn ◽  
Hyun Wook Kang ◽  
...  
Keyword(s):  
Endotracheal Intubation ◽  
Tracheal Stenosis ◽  
Rabbit Model

A Modified Rabbit Model for the Endoscopic Creation and Study of Benign Tracheal Stenosis

2009 ◽  
Vol 119 (S1) ◽  
pp. S95-S95
Author(s):  
Bryan C. Ego-Osuala ◽  
Richard J. Wright ◽  
Duane Sewell ◽  
Tanya K. Meyer
Keyword(s):  
Tracheal Stenosis ◽  
Rabbit Model
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