Tracheal Stenosis Induction: A Comparison of Open Versus Endoscopic Methods in a Rabbit Model

2009 ◽  
Vol 119 (S1) ◽  
pp. S105-S105
Author(s):  
Benjamin J. Wycherly ◽  
Hosai N. Hesham ◽  
Sonya Malekzadeh ◽  
Matthew K. Steehler ◽  
Kevin M. Burke
Keyword(s):  
Tracheal Stenosis ◽  
Rabbit Model ◽  
Open Versus ◽  
Endoscopic Methods

scholarly journals Role of Erythromycin-Regulated Histone Deacetylase-2 in Benign Tracheal Stenosis

2020 ◽  
Vol 2020 ◽  
pp. 1-10
Author(s):  
Zhenjie Huang ◽  
Peng Wei ◽  
Luoman Gan ◽  
Tonghua Zeng ◽  
Caicheng Qin ◽  
...  
Keyword(s):  
Histone Deacetylase ◽  
Tracheal Stenosis ◽  
Type I Collagen ◽  
Rabbit Model ◽  
Immunohistochemical Method ◽  
Type Iii Collagen ◽  
Type I ◽  
Histone Deacetylase 2 ◽  
Budesonide Group

Objective. This study aims to explore the role of erythromycin-regulated histone deacetylase-2 in benign tracheal stenosis. Methods. The rabbit model of tracheal stenosis was established. The rabbits were randomly divided into 8 groups. Histone deacetylase-2 (HDAC2) expression was detected by immunofluorescence. The expression of type I collagen and type III collagen was detected by immunohistochemical method. The expression of TGF-β1, VEGF and IL-8 in serum and alveolar lavage fluid was detected by ELISA. The expression of HDAC2, TGF-β1, VEGF and IL-8 in bronchi of each group was detected by Western blotting method. Results. In Erythromycin (ERY) group, ERY + Budesonide group, ERY + Vorinostat group and ERY + Budesonide + Vorinostat group, the degree of bronchial stenosis was alleviated, and the mucosal epithelium was still slightly proliferated. The effect of ERY combined with other drugs was more obvious. The HDAC2 protein expression increased significantly in ERY group, ERY + Budesonide group and ERY + Budesonide + Vorinostat group and decreased significantly in Vorinostat group, the expression of collagen I and III decreased significantly in ERY group, ERY + Budesonide group and ERY + Budesonide + Vorinostat group (P<0.05). The TGF-β1, IL-8 and VEGF levels decreased significantly in ERY group, ERY + Budesonide group, ERY + Vorinostat group and ERY + Budesonide + Vorinostat group (P<0.05). Conclusions. Erythromycin inhibited inflammation and excessive proliferation of granulation tissue after tracheobronchial mucosal injury by up-regulating the expression of HDAC2, it promoted wound healing and alleviated tracheobronchial stenosis. When combined with budesonide, penicillin and other glucocorticoids and antibiotics, it had a good synergistic effect. However, vorinostat could attenuate erythromycin’s effect by down-regulating the expression of HDAC2. It may have good clinical application prospects in the treatment of tracheal stenosis.

Induction of tracheal stenosis in a rabbit model-endoscopic versus open technique

2011 ◽  
Vol 121 (3) ◽  
pp. 509-514 ◽  
Author(s):  
Matthew K. Steehler ◽  
Hosai N. Hesham ◽  
Benjamin J. Wycherly ◽  
Kevin M. Burke ◽  
Sonya Malekzadeh
Keyword(s):  
Tracheal Stenosis ◽  
Rabbit Model ◽  
Open Technique

scholarly journals Vancomycin-Loaded Polycaprolactone Electrospinning Nanofibers Modulate the Airway Interfaces to Restrain Tracheal Stenosis

2021 ◽  
Vol 9 ◽  
Author(s):  
Yanan Zhao ◽  
Chuan Tian ◽  
Kunpeng Wu ◽  
Xueliang Zhou ◽  
Kexing Feng ◽  
...  
Keyword(s):  
Tracheal Stenosis ◽  
Rabbit Model ◽  
Biological Properties ◽  
Covered Stent ◽  
Metallic Stent ◽  
Electrospinning Technique ◽  
Airway Stent ◽  
Expandable Metallic Stent ◽  
Covered Metallic Stent ◽  
Ft Ir

Site-specific release of therapeutics at the infected trachea remains a great challenge in clinic. This work aimed to develop a series of vancomycin (VA)-loaded polycaprolactone (PCL) composite nanofiber films (PVNF-n, n = 0, 1, and 5, respectively) via the electrospinning technique. The physiochemical and biological properties of PVNF-n were evaluated by a series of tests, such as FT-IR, XRD, SEM-EDS, and antibacterial assay. The PVNF-n samples displayed a typical network structure of fibers with random directions. VA was successfully introduced into the PCL nanofibers and could be sustained and released. More importantly, PVNF-5 showed relatively good antibacterial activity against both methicillin-resistant Staphylococcus aureus (MRSA) and Streptococcus pneumoniae (SPn). Thus, PVNF-5 was covered onto the self-expandable metallic stent and then implanted into a New Zealand rabbit model to repair tracheal stenosis. Compared to a metallic stent, a commercial pellosil matrix–covered stent, and a PVNF-0–covered metallic stent, the PVNF-5–covered airway stent showed reduced granulation tissue thickness, collagen density, α-SMA, CD68, TNF-α, IL-1, and IL-6 expression. In conclusion, this work provides an anti-infection film–covered airway stent that in site restrains tracheal stenosis.

Rabbit model of tracheal stenosis induced by prolonged endotracheal intubation using a segmented tube

2015 ◽  
Vol 79 (12) ◽  
pp. 2384-2388 ◽  
Author(s):  
Hyoung Shin Lee ◽  
Sung Won Kim ◽  
Chulho Oak ◽  
Yeh-Chan Ahn ◽  
Hyun Wook Kang ◽  
...  
Keyword(s):  
Endotracheal Intubation ◽  
Tracheal Stenosis ◽  
Rabbit Model

A Modified Rabbit Model for the Endoscopic Creation and Study of Benign Tracheal Stenosis

2009 ◽  
Vol 119 (S1) ◽  
pp. S95-S95
Author(s):  
Bryan C. Ego-Osuala ◽  
Richard J. Wright ◽  
Duane Sewell ◽  
Tanya K. Meyer
Keyword(s):  
Tracheal Stenosis ◽  
Rabbit Model

Superior Strategy in Benign Tracheal Stenosis Treatment: Surgery or Endoscopy?

2020 ◽  
Author(s):  
Umit Aydogmus ◽  
Argun Kis ◽  
Erhan Ugurlu ◽  
Gokhan Ozturk
Keyword(s):  
Tracheal Stenosis ◽  
Treatment Success ◽  
Segment Length ◽  
Success Rates ◽  
Surgical Methods ◽  
Patient Groups ◽  
Postsurgical Recurrence ◽  
Surgical Treatments ◽  
Endoscopic Methods ◽  
Stenotic Segment

Abstract Introduction Due to the variations in (laryngeal) tracheal stenosis (TS) patient groups, there is still no consensus on which patient should be treated with endoscopy or surgery. The aim of the present study was to generate an algorithm in the light of the related literature and the data obtained from a clinic where both endoscopic and surgical treatments are conducted. Method A retrospective analysis was performed on the data of a total of 56 patients during 2013 to 2019. A total of 38 patients were subject to surgery with 31 as a first treatment option and 7 due to the unsatisfactory results of endoscopic treatments. Endoscopic approaches were tried on a total of 29 patients with 25 as initial treatment and 4 due to postsurgical recurrence. Results Symptomatic full control ratio was determined as 69% with endoscopic treatments, 89.5% in subglottic stenosis (SGS) surgery (n = 19), and 89.5% in trachea surgery (n = 19). However, success rates with no recurrence were determined, respectively, as 40.0, 36.4, and 36.4% for patients subject to dilatation, stent, or T tube treatment. Dilatation was observed to be successful in patients with stenotic segment lengths of less than 1.5 cm (p = 0.02). Failure rates increased in SGS (p = 0.03) and TS (p = 0.12) in the surgical group with increasing stenotic segment length. The presence of comorbidities was not effective on treatment success. Conclusion Endoscopic methods are preferred in cases of web-like stenosis. Surgical methods should first be considered for other patients and endoscopic methods should be used on patients who are not suited for surgery or in cases of postsurgical recurrence.

Surface-modified silicone T-tubes for prevention of tracheal stenosis in a rabbit model

2014 ◽  
Vol 125 (6) ◽  
pp. 1465-1471 ◽  
Author(s):  
Jeong-Seok Choi ◽  
Jae-Yol Lim ◽  
In S. Park ◽  
Si Y. Seo ◽  
Yoon K. Joung ◽  
...  
Keyword(s):  
Tracheal Stenosis ◽  
Rabbit Model ◽  
Surface Modified

scholarly journals Erythromycin combined with corticosteroid reduced inflammation and modified trauma-induced tracheal stenosis in a rabbit model

2018 ◽  
Vol 12 ◽  
pp. 175346661877370 ◽  
Author(s):  
Qin Enyuan ◽  
Xu Mingpeng ◽  
Gan Luoman ◽  
Gan Jinghua ◽  
Li Yu ◽  
...  
Keyword(s):  
Lamina Propria ◽  
Tracheal Stenosis ◽  
Collagen Type ◽  
Quantitative Polymerase Chain Reaction ◽  
Rabbit Model ◽  
Tracheal Epithelium ◽  
Interleukin 17 ◽  
Type I ◽  
Protein Levels ◽  
Tgf Β1

Background: Patients with endotracheal intubation or tracheostomy are subject to benign tracheal stenosis (TS), for which current therapies are unsatisfactory. We conducted a preliminary investigation of drugs and drug combinations for the prevention and treatment of TS in a rabbit model. Methods: Fifty-four rabbits were apportioned into nine groups according to treatment: sham-operated control; untreated TS model; amikacin; budesonide; erythromycin; penicillin; amikacin + budesonide; erythromycin + budesonide; and penicillin + budesonide. TS was induced by abrasion during surgery. The drugs were applied for 7 days before and 10 days after the surgery. Rabbits were killed on the eleventh day. Tracheal specimens were processed for determining alterations in the thicknesses of tracheal epithelium and lamina propria via hematoxylin and eosin. The tracheal mRNA (assessed by real-time quantitative polymerase chain reaction) expressions of the following fibrotic-related factors were determined: transforming growth factor-β1 (TGF- β1), collagen type I (COL1A1), collagen type III (COL3A1), and interleukin-17 (IL-17). The protein levels of TGF-β1, COL1A1, and COL3A1 were determined through immunohistochemistry and integrated optical densities. Results: Compared with all other groups, the untreated TS model had significantly thicker tracheal epithelium and lamina propria, and higher mRNA and protein levels of all targeted fibrotic factors. The mRNA and protein levels of the targeted fibrotic factors in all the drug-treated groups were lower than those of the untreated TS model, and differences were most significant in the erythromycin + budesonide group. Conclusions: Erythromycin combined with budesonide may reduce inflammation and modify fibrosis progression in TS after tracheal injury.

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