Simultaneous quantification of six flavonoids of Rhus verniciflua Stokes using matrix solid‐phase dispersion via high‐performance liquid chromatography coupled with photodiode array detector

2020 ◽  
Vol 43 (23) ◽  
pp. 4281-4288
Author(s):  
HongXia Chen ◽  
Hao Zhou ◽  
Ran Tao ◽  
WenJun Li ◽  
Cheng Zhang Wang
Keyword(s):  
High Performance ◽  
Solid Phase ◽  
Array Detector ◽  
Matrix Solid Phase Dispersion ◽  
Simultaneous Quantification ◽  
Phase Dispersion ◽  
Rhus Verniciflua Stokes ◽  
Rhus Verniciflua ◽  
Photodiode Array Detector ◽  
Photodiode Array

scholarly journals Ultrasound-assisted Matrix Solid Phase Dispersion for the HPLC-DAD analysis of amphenicols in shrimps

2015 ◽  
Vol 2 (1) ◽  
Author(s):  
Victoria F. Samanidou ◽  
Evgenia A. Makrygianni
Keyword(s):  
Solid Phase ◽  
Extraction Procedure ◽  
Array Detector ◽  
The European Union ◽  
Matrix Solid Phase Dispersion ◽  
Phase Dispersion ◽  
Cleanup Efficiency ◽  
Photodiode Array Detector ◽  
Ultrasound Assisted ◽  
The Impact

AbstractUltrasound-assisted matrix solid phase dispersive extraction was applied for the selective isolation and clean-up of three amphenicol antibiotics, chloramphenicol (CAP), thiamphenicol (TAP) and florfenicol (FFC) from shrimp. The target antibiotics were separated on a LiChroCART-LiChrospher® 100 RP-18 (5 μm, 250 × 4 mm) analytical column in less than 9 min, with isocratic elution using a mixture of 70% ammonium acetate (0.05 M) and 30% acetonitrile (v/v). Matrix Solid Phase Dispersion protocol was optimized in terms of extraction sorbent and elution solvent. Two polymer based (Oasis and Nexus) sorbents and one silica based (Lichrolut C18) were compared and different elution solvents such as methanol, acetone, acetonitrile and isopropanol were evaluated based on the achieved recovery rates as well as on the cleanup efficiency. The extraction procedure was performed with and without sonication to evaluate the impact of ultrasounds. TAP and FFC were monitored at 234 nm and CAP at 280 nm by a photodiode array detector. The method was validated according to the European Union Decision 2002/657/EC in terms of linearity, selectivity, stability, accuracy, precision and sensitivity. Detection capability values (CCb) were 64.6 μg/kg for TAP and 1046.8 μg/kg for FFC and 63.8 μg/ kg for CAP.

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