A rapid and efficient extraction method based on industrial MCM‐41‐miniaturized matrix solid‐phase dispersion extraction with response surface methodology for simultaneous quantification of six flavonoids in Pollen typhae by ultra‐high‐performance liquid chromatography

2019 ◽  
Vol 42 (14) ◽  
pp. 2426-2434 ◽  
Author(s):  
Xiaoyan Wang ◽  
Jin Li ◽  
Xuejing Yang ◽  
Xiu‐mei Gao ◽  
Hui Wang ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
High Performance Liquid Chromatography ◽  
Extraction Method ◽  
High Performance ◽  
Solid Phase ◽  
Matrix Solid Phase Dispersion ◽  
Simultaneous Quantification ◽  
Phase Dispersion ◽  
Efficient Extraction ◽  
Mcm 41

Rapid Screening of Oleuropein from Olive Leaves Using Matrix Solid-Phase Dispersion and High-Performance Liquid Chromatography

2014 ◽  
Vol 97 (4) ◽  
pp. 1109-1113 ◽  
Author(s):  
Marzieh Rashidipour ◽  
Rouhollah Heydari ◽  
Alireza Feizbakhsh ◽  
Payman Hashemi
Keyword(s):  
High Performance Liquid Chromatography ◽  
Extraction Method ◽  
High Performance ◽  
Solid Phase ◽  
Soxhlet Extraction ◽  
Rapid Screening ◽  
Olive Leaves ◽  
Matrix Solid Phase Dispersion ◽  
Sorbent Material ◽  
Phase Dispersion

Abstract Matrix solid-phase dispersion (MSPD) methodology as a quick and easy extraction method has been developed to extract oleuropein from Olea europaea leaves. This method has been compared with conventional maceration and Soxhlet extraction (SOXE) methods. Variables affecting MSPD, such as the sorbent material, the ratio of sample to sorbent material, elution solvent, and volume of the elution solvent, have been fully evaluated and optimized. Oleuropein was successfully extracted by silica sorbent with 350 μL of dichloromethane–methanol (70 + 30, v/v) as the elution solvent. The calibration curve showed good linearity (r2 = 0.9985) and precision (RSD <4.5%) in the concentration range of 0.5–100 μg/mL for oleuropein. The LOD and LOQ were 0.14 and 0.5 μg/mL, respectively. The recoveries were in the range of 88.0–94.0% with RSD values ranging from 6.0 to 9.8%. The extraction yields obtained by the MSPD method were comparable with those obtained by the maceration and SOXE methods. The reported MSPD extraction method was revealed to be eco-friendly, simpler, and faster than conventional methods used to quantify oleuropein in O. europaea leaves.

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