Effect of pH and mobile phase additives on the chromatographic behaviour of an amide‐embedded stationary phase: Cyanocobalamin and its diaminemonochloro‐platinum(II) conjugate as a case study

2019 ◽  
Vol 42 (6) ◽  
pp. 1155-1162 ◽  
Author(s):  
Giovanni Ventura ◽  
Cosima Damiana Calvano ◽  
Ilario Losito ◽  
Giuliana Bianco ◽  
Raffaella Pascale ◽  
...  
Keyword(s):  
Stationary Phase ◽  
Mobile Phase ◽  
Effect Of Ph ◽  
Chromatographic Behaviour ◽  
Mobile Phase Additives

Application of ionic liquids as mobile phase additives and surface-bonded stationary phase in liquid chromatography

2009 ◽  
Vol 26 (5) ◽  
pp. 1353-1358 ◽  
Author(s):  
Dandan Han ◽  
Minglei Tian ◽  
Dong Wha Park ◽  
Dae Ki Choi ◽  
Kyung Ho Row
Keyword(s):  
Ionic Liquids ◽  
Liquid Chromatography ◽  
Stationary Phase ◽  
Mobile Phase ◽  
Mobile Phase Additives

Enantioseparation of Cinacalcet, and its Two Related Compounds by HPLC with Self-Made Chiral Stationary Phases and Chiral Mobile Phase Additives

2019 ◽  
Vol 15 (2) ◽  
pp. 200-209
Author(s):  
Canyu Yang ◽  
Ji Li ◽  
Yanyun Yao ◽  
Chen Qing ◽  
Baochun Shen
Keyword(s):  
Stationary Phase ◽  
Mobile Phase ◽  
High Performance ◽  
Chiral Stationary Phase ◽  
Stationary Phases ◽  
Chiral Stationary Phases ◽  
Starting Material ◽  
Mobile Phase Additives ◽  
Chiral Mobile Phase Additives ◽  
Related Compounds

Background: Cinacalcet is one of the second-generation calcimimetics which consists of a chiral center. The pharmacological effect of R-cinacalcet is 1000 times greater than that of the Scinacalcet. As mentioned in many literatures, 1-(1-naphthyl)ethylamine is used as the starting material for the synthesis of cinacalcet. The absolute structure of cinacalcet is influenced by the starting materials. Methods: We present the chiral separation of cinacalcet and its starting material, 1-(1-naphthyl) ethylamine along with one of its intermediates, N-(1-(naphthalen-1-yl) ethyl)-3- (3-(trifluoromethyl) phenyl) propanamide by high-performance liquid chromatography with chiral stationary phase and chiral mobile phase additives. Results: On vancomycin and cellulose tri 3,5-dimethylphenylcarbamate) chiral stationary phase, cinacalcet and 1-(1-naphthyl)ethylamine achieved enantioseparation under normal phase with addition of triethylamine additives, respectively. Meanwhile, 1-(1-naphthyl)ethylamine and N-(1-(naphthalen-1- yl)ethyl)-3-(3-(trifluoromethyl) phenyl) propanamide achieved enantioseparation on 1-napthalene vancomycin chiral stationary phase using D-tartaric acid, diethyl L-tartrate and diethyl D-tartrate as chiral mobile phase additives. Conclusion: The chiral recognition in our experiment was based on the hydrogen-bonding, dipoledipole and π-π interactions among the solutes, chiral stationary phases and chiral mobile phase additives. In addition, the space adaptability of chiral stationary phases also affected the separation efficacy.

Separation of fudosteine on an achiral C18column by HPLC with chiral ligand-exchange selectors as mobile phase additives

2008 ◽  
Vol 20 (4) ◽  
pp. 585-594 ◽  
Author(s):  
J. Ma ◽  
J. Cao ◽  
M. J. Ding ◽  
L. H. Yuan ◽  
M. J. Zhai ◽  
...  
Keyword(s):  
Ligand Exchange ◽  
Mobile Phase ◽  
Chiral Ligand ◽  
Mobile Phase Additives ◽  
Chiral Ligand Exchange
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