Determination of five sesquiterpenoids in Xingnaojing injection by quantitative analysis of multiple components with a single marker

2015 ◽  
Vol 38 (19) ◽  
pp. 3313-3323 ◽  
Author(s):  
Weidong Pan ◽  
Lixin Yang ◽  
Weihong Feng ◽  
Limei Lin ◽  
Chun Li ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Multiple Components ◽  
Single Marker ◽  
Xingnaojing Injection

scholarly journals Simultaneous determination of five diuretic drugs using quantitative analysis of multiple components by a single marker

BMC Chemistry ◽  
2021 ◽  
Vol 15 (1) ◽  
Author(s):  
Fuchao Chen ◽  
Baoxia Fang ◽  
Peng Li ◽  
Sicen Wang
Keyword(s):  
Quantitative Analysis ◽  
Simultaneous Determination ◽  
Dihydrogen Phosphate ◽  
Column Temperature ◽  
Multiple Components ◽  
External Standard Method ◽  
Significant Difference ◽  
Diuretic Drugs ◽  
Single Marker

Abstract Background Loop diuretics are commonly used in clinical practice to manage high fluid loads and to control fluid balance. In this paper, a novel quantitative analysis method for multiple components with a single marker (QAMS) was developed for the simultaneous determination of 5 diuretic drugs furosemide, torasemide, azosemide, etacrynic acid, and bumetanide, by HPLC. Qualitative analysis was performed using relative retention time and ultraviolet (UV) spectral similarity as the double indicator. The QAMS method was conducted with etacrynic acid as an internal reference substance. The quantities of the other four diuretics were calculated by using the relative correction factors for etacrynic acid. The quantities of the 5 diuretic drugs were also determined by the external standard method (ESM). Chromatographic separation was achieved on a Shimadzu HC-C18 column (150 mm × 4.6 mm, 5 µm) using 50 mM potassium dihydrogen phosphate (pH adjusted to 4.0 with phosphoric acid) with acetonitrile (64:36, v/v) as the mobile phase at a flow rate of 1.0 mL/min and a column temperature of 30 ℃. Results Under these conditions, the 5 diuretic drugs were well separated, showing linear relationships within certain ranges. The quantitative results showed that there was no significant difference between the QAMS and ESM methods. Conclusions Overall, the HPLC-QAMS analytical scheme established in this study is a simple, efficient, economical, and accurate method for the quantitative evaluation of 5 diuretic drugs.

scholarly journals Simultaneous Determination of 25 Ginsenosides by UPLC-HRMS via Quantitative Analysis of Multicomponents by Single Marker

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Xiujuan Jia ◽  
Chenxing Hu ◽  
Xuepeng Zhu ◽  
Ye Yuan ◽  
Yifa Zhou
Keyword(s):  
Quantitative Analysis ◽  
Qualitative And Quantitative Analysis ◽  
High Throughput Analysis ◽  
Multiple Components ◽  
External Standard Method ◽  
External Standard ◽  
Single Marker ◽  
Ginsenoside Content ◽  
Elution Gradient

A method using UPLC-HRMS has been developed for a rapid, simultaneous qualitative and quantitative analysis of twenty-five ginsenosides. Chromatographic separation was achieved on a C18 analytical column with an elution gradient comprising 0.1% aqueous formate/acetonitrile as the mobile phase. HRMS detection acquired full mass data for quantification and fullms-ddms2 (i.e., data-dependent scan mode) yielded product ion spectra for identification. Furthermore, quantitative analysis of multiginsenosides by single marker (QAMS) was developed and validated using a relative correction factor. Under optimal conditions, we could simultaneously separate eight groups of isomers of the 25 ginsenosides. Good linearity was observed over the validated concentration range for each analyte (r2 > 0.9924), showing excellent sensitivity (LODs, 0.003–0.349 ng/mL) and lower limit quantification (LOQs, 0.015–1.163 ng/mL). The LC-MS external standard method (ESM) and QAMS were compared and successfully applied to analyze the ginsenoside content from Panax ginseng roots. Overall, our UPLC-HRMS/QAMS approach provides high precision, stability, and reproducibility and can be used for high-throughput analysis of complex ginsenosides and quantitative analysis of multiple components and quality control of traditional Chinese medicines (TCM).

scholarly journals Simultaneous determination of 5 diuretic drugs using an HPLC method by quantitative analysis of multiple components by a single marker

2021 ◽  
Author(s):  
Fuchao Chen ◽  
Baoxia Fang ◽  
Peng Li ◽  
Sicen Wang
Keyword(s):  
Quantitative Analysis ◽  
Simultaneous Determination ◽  
Dihydrogen Phosphate ◽  
Column Temperature ◽  
Multiple Components ◽  
External Standard Method ◽  
Significant Difference ◽  
Diuretic Drugs ◽  
Single Marker

Abstract Background: Loop diuretics are commonly used in clinical practice to manage high fluid loads and to control fluid balance. In this paper, a novel quantitative analysis method for multiple components with a single marker (QAMS) was developed for the simultaneous determination of 5 diuretic drugs furosemide, torasemide, azosemide, etacrynic acid, and bumetanide, by HPLC. Qualitative analysis was performed using relative retention time and ultraviolet (UV) spectral similarity as the double indicator. The QAMS method was conducted with etacrynic acid as an internal reference substance. The quantities of the other four diuretics were calculated by using the relative correction factors for etacrynic acid. The quantities of the 5 diuretic drugs were also determined by the external standard method (ESM). Chromatographic separation was achieved on a Shimadzu HC-C18 column (150 mm× 4.6 mm, 5µm) using 50 mM potassium dihydrogen phosphate (pH adjusted to 4.0 with phosphoric acid) with acetonitrile (64:36, v/v) as the mobile phase at a flow rate of 1.0 mL/min and a column temperature of 30 ℃. Results: Under these conditions, the 5 diuretic drugs were well separated, showing linear relationships within certain ranges. The quantitative results showed that there was no significant difference between the QAMS and ESM methods. Conclusions: Overall, the HPLC-QAMS analytical scheme established in this study is a simple, efficient, economical, and accurate method for the quantitative evaluation of 5 diuretic drugs.

scholarly journals Exploitation of HPLC Analytical Method for Simultaneous Determination of Six Principal Unsaturated Fatty Acids in Oviductus Ranae Based on Quantitative Analysis of Multi-Components by Single-Marker (QAMS)

Molecules ◽  
2021 ◽  
Vol 26 (2) ◽  
pp. 479
Author(s):  
Shihan Wang ◽  
Yuanshuai Gan ◽  
Hong Kan ◽  
Xinxin Mao ◽  
Yongsheng Wang
Keyword(s):  
Fatty Acids ◽  
Quantitative Analysis ◽  
Unsaturated Fatty Acids ◽  
Internal Standard ◽  
Active Components ◽  
Reliable Determination ◽  
External Standard Method ◽  
Single Marker ◽  
Oviductus Ranae

As one of the featured products in northeast China, Oviductus Ranae has been widely used as a nutritious food, which contains a variety of bioactive unsaturated fatty acids (UFAs). It is necessary to establish a scientific and reliable determination method of UFA contents in Oviductus Ranae. In this work, six principal UFAs in Oviductus Ranae, namely eicosapentaenoic acid (EPA), linolenic acid (ALA), docosahexaenoic acid (DHA), arachidonic acid (ARA), linoleic acid (LA) and oleic acid (OA), were identified using UPLC-MS/MS. The UFAs identified in Oviductus Ranae were further separated based on the optimized RP-HPLC conditions. Quantitative analysis of multi-components by single-marker (QAMS) method was implemented in content determination of EPA, ALA, DHA, ARA and OA, where LA was used as the internal standard. The experiments based on Taguchi design verified the robustness of the QAMS method on different HPLC instruments and chromatographic columns. The QAMS and external standard method (ESM) were used to calculate the UFA content of 15 batches of Oviductus Ranae samples from different regions. The relative error (r < 0.73%) and cosine coefficient showed that the two methods obtained similar contents, and the method validations met the requirements. The results showed that QAMS can comprehensively and effectively control the quality of UFAs in Oviductus Ranae which provides new ideas and solutions for studying the active components in Oviductus Ranae.

New Determination Method of Two Bioactive Saponins in Lysimachia capillipes Hemsl. by Quantitative Analysis of Multi-Components by Single-Marker

2021 ◽  
Author(s):  
Xiaoyong Zhang ◽  
Xuezhao Chen ◽  
Juan Jin ◽  
Minghua Gong ◽  
Qiang He ◽  
...  
Keyword(s):  
Quality Control ◽  
Quantitative Analysis ◽  
High Performance ◽  
Reference Standards ◽  
Bioactive Components ◽  
Evaporative Light Scattering Detector ◽  
Evaporative Light Scattering ◽  
External Standard ◽  
Single Marker

Abstract Capilliposide B (CPS-B) and Capilliposide C (CPS-C), as the key components in Lysimachia capillipes Hemsl., increasingly aroused the interest and research concern of many researchers due to the good bioactivities. Nowadays, the reference standards of CPS-B and CPS-C yield were very limited. Due to the deficit of reference standards, the determination could be difficult to carry out, and the quality control and evaluation would be restrained afterwards. To solve this urgent problem, a quantitative analysis of multi-components by single-marker (QAMS) method was proposed and established based on high-performance liquid-chromatography tandem evaporative light-scattering detector. In this QAMS method, the content of the two bioactive components could be calculated by buddlejasaponin IV, which is applied as an external standard and readily obtained. And the methodological experiments were evaluated and indicated accuracy, stability and feasibility of this QAMS method. Therefore, in this study, this built method would properly meet the requirement of determination of CPS-B, CPS-C and quality control of the L. capillipes Hemsl. plant.

Qualitative analysis of Psoraleae Fructus by HPLC-DAD/TOF-MS fingerprint and quantitative analysis of multiple components by single marker

2017 ◽  
Vol 32 (2) ◽  
pp. e4059 ◽  
Author(s):  
Lianjun Luan ◽  
Xiaoyu Shen ◽  
Xuesong Liu ◽  
Yongjiang Wu ◽  
Manliang Tan
Keyword(s):  
Quantitative Analysis ◽  
Qualitative Analysis ◽  
Multiple Components ◽  
Single Marker ◽  
Tof Ms

scholarly journals Quality Evaluation of Polar and Active Components in Crude and ProcessedFructus Corniby Quantitative Analysis of Multicomponents with Single Marker

2016 ◽  
Vol 2016 ◽  
pp. 1-13 ◽  
Author(s):  
Yuhong Jiang ◽  
Hui Chen ◽  
Liling Wang ◽  
Jing Zou ◽  
Xiao Zheng ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Active Components ◽  
Internal Reference ◽  
External Standard Method ◽  
Significant Difference ◽  
External Standard ◽  
Single Marker ◽  
Quantitative Results ◽  
Processed Products

Objective. To develop a quantitative analysis of multicomponents by single-marker (QAMS) method for the simultaneous determination of polar active components inFructus Corni.Methods. Loganin was selected as the internal reference, and the relative correction factors (RCFs) of gallic acid, 5-hydroxymethyl-2-furfural, morroniside, sweroside, cornin, 7α-O-methylmorroniside, 7β-O-methylmorroniside, 7α-O-ethylmorroniside, 7β-O-ethylmorroniside, and cornuside were established. The contents of multicomponents were then calculated based on their RCFs, respectively. Contents of the 11 components were also calculated by external standard method and compared with those of the QAMS method.Results.The contents of the 11 components in 21 crude and 10 processedFructus Corniproducts were measured. No significant difference was found in the quantitative results of the QAMS and external standard methods.Conclusion. QAMS could serve as an accurate and convenient method in determining the polar and active components inFructus Corniand its processed products.

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