Rapid preparative isolation of erythrocentaurin fromEnicostemma littoraleby medium-pressure liquid chromatography, its estimation by high-pressure thin-layer chromatography, and its α-amylase inhibitory activity

2015 ◽  
Vol 38 (4) ◽  
pp. 592-598 ◽  
Author(s):  
Naila Hassan ◽  
Javed Ahamad ◽  
Saima Amin ◽  
Mohd Mujeeb ◽  
Showkat R. Mir
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Inhibitory Activity ◽  
Preparative Isolation ◽  
Medium Pressure ◽  
Medium Pressure Liquid Chromatography ◽  
Layer Chromatography

Determination of Zearalenone in Cornflakes and Other Corn-Based Foods by Thin Layer Chromatography, High Pressure Liquid Chromatography, and Gas-Liquid Chromatography/High Resolution Mass Spectrometry

1978 ◽  
Vol 61 (3) ◽  
pp. 593-600
Author(s):  
Peter M Scott ◽  
Thavil Panalaks ◽  
Sri Kanhere ◽  
Walter F Miles
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
High Resolution ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Pressure Liquid Chromatography ◽  
Limit Of Detection ◽  
Gas Liquid Chromatography ◽  
Natural Occurrence ◽  
Layer Chromatography

Abstract A simple cleanup procedure based on pH adjustments was used to obtain extracts of corn foods. The method gave good recoveries of zearalenone determined by thin layer chromatography (TLC) and high pressure liquid chromatography (HPLC). As little as 5 ng zearalenone was detected by TLC, using Fast Violet B Salt as the spray reagent; the lower limit of detection in cornflakes was about 20 ≧kg. With HPLC on Spherisorb silica (5 ≧m) and detection by fluorescence at an excitation maximum of 310 nm as little as 5 ≧g zearalenone/kg cornflakes could be determined. While the TLC method was also applicable to corn chips, cornmeal, popcorn, and frozen corn, an interference was observed in HPLC of the latter 3 products. This interference was separated from zearalenone by adding a second HPLC analytical column (Spherisorb ODS). Gas-liquid chromatography coupled with mass spectrometric single ion monitoring at high resolution, although of limited availability, was shown to be the most sensitive and selective method for determining zearalenone in corn foods. The natural occurrence of zearalenone in a sample of cornflakes (13–20 ≧/kg) was demonstrated by all 3 detection procedures.

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