Application and recovery of ionic liquids in the preparative separation of four flavonoids fromRhodiola roseaby on-line three-dimensional liquid chromatography

2014 ◽  
Vol 37 (17) ◽  
pp. 2314-2321 ◽  
Author(s):  
Shufeng Ma ◽  
Liming Hu ◽  
Chaoyang Ma ◽  
Wenping Lv ◽  
Hongxin Wang
Keyword(s):  
Ionic Liquids ◽  
Liquid Chromatography ◽  
Three Dimensional ◽  
Preparative Separation ◽  
On Line

Design and calibration of an IVEM for general 3-dimensional imaging of non-diffracting materials

1992 ◽  
Vol 50 (2) ◽  
pp. 1040-1041
Author(s):  
Neil Rowlands ◽  
Jeff Price ◽  
Michael Kersker ◽  
Seichi Suzuki ◽  
Steve Young ◽  
...  
Keyword(s):  
3D Imaging ◽  
Tomographic Reconstruction ◽  
Three Dimensional ◽  
3 Dimensional ◽  
3D Microstructure ◽  
Image Translation ◽  
Digital Correlation ◽  
On Line ◽  
Mechanical Stage ◽  
Projection Angle

Three-dimensional (3D) microstructure visualization on the electron microscope requires that the sample be tilted to different positions to collect a series of projections. This tilting should be performed rapidly for on-line stereo viewing and precisely for off-line tomographic reconstruction. Usually a projection series is collected using mechanical stage tilt alone. The stereo pairs must be viewed off-line and the 60 to 120 tomographic projections must be aligned with fiduciary markers or digital correlation methods. The delay in viewing stereo pairs and the alignment problems in tomographic reconstruction could be eliminated or improved by tilting the beam if such tilt could be accomplished without image translation.A microscope capable of beam tilt with simultaneous image shift to eliminate tilt-induced translation has been investigated for 3D imaging of thick (1 μm) biologic specimens. By tilting the beam above and through the specimen and bringing it back below the specimen, a brightfield image with a projection angle corresponding to the beam tilt angle can be recorded (Fig. 1a).

Application of on-line two-dimensional liquid chromatography in comparative proteome analysis of antlers with different growing stages

2010 ◽  
Vol 28 (2) ◽  
pp. 146-151 ◽  
Author(s):  
Liang GAO ◽  
Xiaoqiang QIAO ◽  
Zhen LIANG ◽  
Lihua ZHANG ◽  
Yushu HUO ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Proteome Analysis ◽  
Two Dimensional ◽  
On Line
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